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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtttctacttgttcaggataattatacaagcggtgaactcaggcccaatttaactccatctaatctgcag

Basic information

species Zea mays
transcript GRMZM2G381051_T04
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G381051_T01 (Zea mays), 5'ss of exon 7
lower sequence: LOC_Os05g03480.4 (Oryza sativa), 5'ss of exon 8
GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtttctacttgttcaggataattatacaagcggtgaactcaggcccaatttaactcc--atctaatctgcag-----------
|||||||||||| |||||||||||||| ||||||||||||||||| |||||||||| |  ||||| ||    |||||||  | |  ||   || | |          |||  | |  |||| |  |  |            
GTTCAGTACTGCACAGAAGTTGGACAAGCTTGGCATGAGAGGAAGTGACACgtatgtt--ttctatttaag-aggataaccaaaagagt--tgcattagattttgcattagattcgtatcttacatatatttttgacacag

upper sequence: GRMZM2G381051_T01 (Zea mays), 5'ss of exon 7
lower sequence: AT3G45300.1 (Arabidopsis thaliana), 5'ss of exon 9
GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtttctacttgttcaggataattatacaagcggtgaactcaggcccaatttaactccatctaa-tctgcag--------------------
 ||||||||||||||||| ||||||||||| || ||| | |||||||| |||||     |  | | |   ||| |||||  |||   | | | || | |  |||  ||| |||   |||   |||  |                     
ATTCAGTACTGCTCAGAAATTGGACAAACTAGGAATGCGGGGAAGCGATACgta----atccccattctctcaagataaaaatatctgtg-taaattaaatccctttttcactgtctctttctctctaacatctgtctctcctgagcag

upper sequence: GRMZM2G381051_T01 (Zea mays), 5'ss of exon 7
lower sequence: Vv06s0004g03140.t01 (Vitis vinifera), 5'ss of exon 9
GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatg---------------gtgtttctacttgttcaggataattatacaagcggtgaactcaggcccaatttaactccatctaatctgcag
 ||||||||||||||||| || ||||||||||| ||| ||||||| |||||||| |                || | |  ||| ||        || |  ||  || ||    | |   | ||| | |  | | ||  |||||
ATTCAGTACTGCTCAGAAATTAGACAAACTTGGGATGCGAGGAAGTGACACgtaagcctcattactacagcatgctcccccttttttctctcttttctgaaactggagaggatatgataattttcatt--aacaaaattgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148952108|gb|EC897900.2|EC897900
EST:     GGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: GGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
EST: gi|148928298|gb|EE284912.2|EE284912
EST:     TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
EST: gi|211288131|gb|FL391420.1|FL391420
EST:     TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
EST: gi|148949902|gb|EC895400.2|EC895400
EST:     GCATGAGAGGAAGCGAACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCG
genomic: GCATGAGAGGAAGCG-ACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCG
EST: gi|31352282|gb|CD436639.1|CD436639
EST:     TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
EST: gi|60356707|gb|DN223680.1|DN223680
EST:     AGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: AGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
EST: gi|40333885|gb|CK367955.1|CK367955
EST:     TTCAGTACTGCTCATAAGTTGGACAAACTTGGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
EST: gi|37394249|gb|CF634396.1|CF634396
EST:     TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACAC                         GTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG
genomic: TTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 65

GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGGTGAAGAAGGCAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1177

GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGGTGAAGAAGGCAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 504

GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGGTGAAGAAGGCAAAG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 89

GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtt ... taatctgcagGTGTGAGCTTGTTTTTGAGAACTGCTTTGTGCCATGCGAAAATGTCCTCGGTGAAGAAGGCAAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTTCAGTACTGCTCAGAAGTTGGACAAACTTGGCATGAGAGGAAGCGACACgtatggtgtttctacttgttcaggataattatacaagcggtgaactcaggcccaatttaactccatctaatctgcag

- - - - - - - AGAAGT
- - - - - - - - - - - - - - - - - - GAGAGG
- - - - - - - - - - - - - - - - - -  AGAGGA