Skip to another splice site:
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 5'  3'   		2  
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 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTCATCAAGGATCAAATGGAACTTTACCAAGTTTCTGGTGGACAAAGATGGGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttccctctctgtctctgtctctctctccctctccagacgtctgcctacgttttctttttgatgcatggattcgtgtttcttcgactctgcag

Basic information

species Zea mays
transcript GRMZM2G329144_T01
intron # 5
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|14243063|gb|BG840760.2|BG840760
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|211520783|gb|FL373488.1|FL373488
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAANNNNNNNGTGAGCTTGTCTCG
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTT-GTGAGCTTGTCTCG
EST: gi|28983489|gb|BQ538710.2|BQ538710
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|10191547|gb|BE761623.1|BE761623
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCCTGAGGAATAATTTTTTGTGA
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGA
EST: gi|101391621|gb|EB817747.1|EB817747
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|211470404|gb|FL454220.1|FL454220
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTT-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|13381984|gb|BG458659.1|BG458659
EST:     GGAAGGTCATCGAGAGATACGGAGCCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|21480775|gb|BQ577458.1|BQ577458
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|87157407|gb|DY402196.1|DY402196
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|32841096|gb|CD980777.1|CD980777
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTG-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|32841256|gb|CD980937.1|CD980937
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTG-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|60356015|gb|DN222988.1|DN222988
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|67011682|gb|CO440431.1|CO440431
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|60358353|gb|DN225326.1|DN225326
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|37379775|gb|CF626646.1|CF626646
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTT-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|37381108|gb|CF627414.1|CF627414
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTT-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|211451170|gb|FL456365.1|FL456365
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTT-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|18180377|gb|BM381587.1|BM381587
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|211007518|gb|FL457984.1|FL457984
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|32841646|gb|CD981327.1|CD981327
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAA-TTTTGGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|26558316|gb|CA830551.1|CA830551
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|32841499|gb|CD981180.1|CD981180
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTG-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|32846828|gb|CD986509.1|CD986509
EST:     GGAAGGTCATCGAGAGATACGGAACCTCAACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTG-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|51592947|gb|CV072093.1|CV072093
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|32841070|gb|CD980751.1|CD980751
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTG-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|47961660|gb|CN844369.1|CN844369
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTT-GTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|61236533|gb|DN586283.1|DN586283
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGCGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|16379741|gb|BI993126.1|BI993126
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCT-GAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|9953562|gb|BE640145.1|BE640145
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|16926510|gb|BM079578.1|BM079578
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|16926216|gb|BM079284.1|BM079284
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|13490714|gb|BG517478.1|BG517478
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|33098044|gb|CF058004.1|CF058004
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAA-TTTTGGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|10191601|gb|BE761677.1|BE761677
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|60341752|gb|DN208725.1|DN208725
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|18180755|gb|BM381965.1|BM381965
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
EST: gi|211230691|gb|FL436009.1|FL436009
EST:     GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAG                         AAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT
genomic: GGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 470

GTCATCAAGGATCAAATGGAACTTTACCAAGTTTCTGGTGGACAAAGATGGGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGTCTCATCCACCACACACTTGCAGCAGAAATAGGCACTTGCTGAAATATTT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 372

GTCATCAAGGATCAAATGGAACTTTACCAAGTTTCTGGTGGACAAAGATGGGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGTCTCATCCACCACACACTTGCAGCAGAAATAGGCACTTGCTGAAATATTT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 251

GTCATCAAGGATCAAATGGAACTTTACCAAGTTTCTGGTGGACAAAGATGGGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGTCTCATCCACCACACACTTGCAGCAGAAATAGGCACTTGCTGAAATATTT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 686

GTCATCAAGGATCAAATGGAACTTTACCAAGTTTCTGGTGGACAAAGATGGGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttcc ... gactctgcagAAGGACATCCAGAAGGCCCTTGAGGAATAATTTTTTGTGAGCTTGTCTCGTCTCATCCACCACACACTTGCAGCAGAAATAGGCACTTGCTGAAATATTT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTCATCAAGGATCAAATGGAACTTTACCAAGTTTCTGGTGGACAAAGATGGGAAGGTCATCGAGAGATACGGAACCTCGACAGCTCCAATGGCAATTGAGgtctccttccctctctgtctctgtctctctctccctctccagacgtctgcctacgttttctttttgatgcatggattcgtgtttcttcgactctgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTC