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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttattacttctctgtttgaaaacatttgttctgtttaagttctaagctatgcatttgtactgtcaagttaaattcggatgtttctgccataattg...

Basic information

species Zea mays
transcript GRMZM2G161969_T01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G161969_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os07g22720.2 (Oryza sativa), 5'ss of exon 10
GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttattacttctctgtttgaaaacatttgttctgtttaagttctaagctatgcatttgtactgtcaagttaaattcg-gatgtttctgccataattg--
|| ||||| || ||||| || || ||||||||||||||||| |||||||||||||||||||||||||||||||||    | | ||||     | |     |       |     ||     |    ||  | ||  | |    |  |||||     |  |   ||    | |    
GTTCCACTGTCGAGTGTAAAGGGAACTGGTCCTGATGGGCGCATTTTGAAGGCAGACATTGAAGATTACTTGGgta---tgtcactttctcctatagttcccccccccccccccaaaaaaaataaaataaaattaaaataaataaataaataaataaaataaatgatgaactctat

upper sequence: GRMZM2G161969_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os02g01500.1 (Oryza sativa), 5'ss of exon 10
GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttattacttct---ctgtttgaaaacatttgttctgtttaagttctaagctatgcatttgtactgtcaagttaaattcggatg-tttctgccataattg
|| ||||| ||||| || |  || ||||||||||||||||| ||||||||||| |||||||||||||||||||||  |   || ||  |   |  |       | ||   ||| | |   | ||    ||   ||     |||    |  |||| |   |   |  |   |||    
GTTCCACTGTCAAGCGTAATGGGAACTGGTCCTGATGGGCGAATTTTGAAGGCGGACATTGAAGATTACTTGGgtatgccctttctcttgtcccctccaccccccttctctctctctttctcctcccttaatgatgaaatctgcgtggaaaaatgcaattaactagttttata----

upper sequence: GRMZM2G161969_T01 (Zea mays), 5'ss of exon 5
lower sequence: Vv11s0016g01870.t01 (Vitis vinifera), 5'ss of exon 10
GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttattacttctctgtttgaaaacatttgttctgtttaagttctaagctatgcatttgtactgtcaagttaaattcggatgtttctgccataattg-
|| || ||   |||  | ||||| || ||||||||||| || ||| ||||||| || ||||||||||| ||||||  | | ||   | | || ||    | |||  | |||||       | |   ||  |  |  |   ||||     ||   ||   | ||| | ||| |  
GTTCCTCTTCAAAGCATCAAAGGGACGGGTCCTGATGGACGCATTGTGAAGGCTGATATTGAAGATTATTTGGgtatgcttttctctatgtgcttttgcagatt-atcctgttacttgcttgattaatttagctagataatcaacaattatggagacaatgctgtcttaagtac

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|20802763|gb|BQ293813.1|BQ293813
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|32932090|gb|CF036902.1|CF036902
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGGGGCTTTTGCGGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|32933945|gb|CF038757.1|CF038757
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|21809700|gb|BQ668018.1|BQ668018
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGTAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|32930018|gb|CF034830.1|CF034830
EST:     ACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: ACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|166511857|gb|EG058331.1|EG058331
EST:     TTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|32934396|gb|CF039208.1|CF039208
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|22472155|gb|BU036635.1|BU036635
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|20802898|gb|BQ293948.1|BQ293948
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
EST: gi|20208122|gb|BQ134196.1|BQ134196
EST:     TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGG                         CCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG
genomic: TACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 133

GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTGATATTCCAAATGCACAGATAAGAAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 185

GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTGATATTCCAAATGCACAGATAAGAAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 165

GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTGATATTCCAAATGCACAGATAAGAAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 249

GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttatt ... gcttctgtagCCAAGGGTGGGCTGAGGGAGGCTTTTGCAGCTCCAGGGTTAGGTTACGTTGATATTCCAAATGCACAGATAAGAAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTCCCACTATCAAGTGTGAAAGGTACTGGTCCTGATGGGCGTATTTTGAAGGCAGACATTGAAGATTACTTGGgtgagttattacttctctgtttgaaaacatttgttctgtttaagttctaagctatgcatttgtactgtcaagttaaattcggatgtttctgccataattg

- - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA