ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaacatctgtttggcttccttttcataatttaaacatgtttttttttattgtatattgccgattgcctgatttgtcttccaaaagttatttgg...

Basic information

species	Zea mays
transcript	GRMZM2G158162_T03
intron #	5
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G158162_T03 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os07g48200.1 (Oryza sativa), 5'ss of exon 5

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaacatctgtttggcttccttttcataatttaaacatgtttttttttattgtatattgccgattgcctgatttgtcttccaaaagttatttgg--
|||||||||||| || || ||  |||| |||||||| || || || |||||||||||||||||||||||||||||||| |||||||| ||||| ||||||  |||    |         ||  |  |  |   |        |    ||||| ||| | || ||   |  ||||||| || |       | || |    
TTCAAATTCCACCATTGTCCCCCTGTTTGAGAAGGTTCTAAGCGCCAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTACCAAAAGCCTGTGCGGAGgtatttttt-tgttcacaaccttttgtggttctgcctcctgttaataaaattttagtgt-tgttacctcattactgatttatcgtttttgtatcatattccc

upper sequence: GRMZM2G158162_T03 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA14G38490.1 (Glycine max), 5'ss of exon 2

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaacttt----------aacatctgtttggcttccttttcata-atttaaacatgtttttttttattgtatattgccgattgcctgat-ttgtcttccaaaagttatttgg---------------------------------------------------------
                 | |||||||| ||||||||  ||||||| ||||||||||| || || || || |||||||| |||||| ||||||| ||||||    |          || || | | |   |   ||   | | || || |     || |||| | |   | ||    |  |    || |  ||  ||   | ||                                                               
-----------------TGCCATTGTTTGAGAAGGTATTGAGTGCCAGTGATGCAGGTCGAATTGGTCGACTTGTTCTCCCAAAATCTTGTGCTGAGgtatgcctctattaatgaaatatttatctcaatcttttagtacacatgtatatgcagttcttttcaataactttttattaaagaaaaatatcatcaccctgtaattgcagcacatttaatatgacaatttgtacctgaatttcaggcttactttcctcctatttcacag

upper sequence: GRMZM2G158162_T03 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA18G05840.1 (Glycine max), 5'ss of exon 7

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaacatctgtttggcttccttttcataatttaaacatgtttttttttattgtatattgccgattgcctgatttgtcttccaaaagttatttgg
||  || |||||  | || ||||| || ||||||||  ||||||| ||||||||||| || || || || |||||||| |||||||| ||||| ||||||  |||    | || ||  |||    ||  |||| | | |   |     | || |   |  ||   |||     |  || | |   ||    |     
TTTGAAATCCACTGTAGTGCCATTATTTGAGAAGGTGTTGAGTGCCAGTGATGCAGGTCGAATTGGCCGTCTTGTTCTCCCAAAAGCCTGTGCTGAGgtatttttggagtttgattttctttgaatttgtaatatgatctcttcaccctgtaat---tgctgaacattatacaaactggcatgtgaattgcag----

upper sequence: GRMZM2G158162_T03 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA02G40280.1 (Glycine max), 5'ss of exon 4

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgta----------actttaacatctgtttggcttccttttcata---atttaaacatgtttttttttattgtatattgccgattgcctgatttgtcttccaaaagttatttgg----------------------------------------------------------
||  || || || || || |||||||||||||||||  ||||||| || |||||||| || || || || |||||||| |||||| |||| || ||||||          | |||||  | | | |   | | |||   ||    ||||  || ||| ||||  |||   ||||  |           |  ||  ||   | ||                                                                
TTTGAAGTCTACTATAGTGCCATTGTTCGAGAAGGTATTGAGTGCCAGCGATGCAGGTCGAATTGGTCGACTTGTTCTCCCAAAATCTTGCGCTGAGgtatggctctattaatttaatctttatctcaatccttttgtgtacatgtttacgca-gttcttttaaattacttattatcagagaaaaatatcatcaccctgtaattgcagcacatttaatctgacaattttttaccggaatttcaggcttactttcctcctatttcacag

upper sequence: GRMZM2G158162_T03 (Zea mays), 5'ss of exon 5
lower sequence: AT2G30470.1 (Arabidopsis thaliana), 5'ss of exon 5

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaacatctgtttggc--ttccttttcataatttaaacatgtttttttttattgtatattgccgattgc-ctgatttgtcttccaaaagttatttgg
||  ||||  |  || || ||  | || |||||    || ||||| |||||||| || ||||| || || || |||||||||||||| ||||| ||||||| |||  |||   | |||   |  |||||| |    |  | || |||| |    || |  | ||  |  |||  || | | |                  
TTTGAATTTGAACATTGTACCTCTCTTTGAGAAAACTCTTAGTGCCAGTGATGCTGGTCGCATTGGTCGTCTAGTTCTTCCAAAAGCCTGTGCAGAGgtaaatttcccattcctatggtgatgtcttttcttgtccttgaaatattttgtagagttatg-actgatgtctgcattggtatatag----------------

upper sequence: GRMZM2G158162_T03 (Zea mays), 5'ss of exon 5
lower sequence: Vv06s0004g08100.t01 (Vitis vinifera), 5'ss of exon 5

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaacttt--aacatctgtttggcttccttttcataatttaaacatgtttttttttattgtatattgc-cgattgcctgatttgtcttccaaaagt-tatttgg-------------
||  |||||||| || || ||||| ||||||||||| |||||||| |||||||| || || || || ||  | || || |||||||| ||||| || |||| |||  || |   ||    ||   |     |  ||     ||| |   || | || | ||||    |   |  |   ||   || || | || ||    |             
TTTGAATTCCACCATTGTGCCATTATTCGAGAAGGTTCTGAGTGCTAGTGATGCTGGTCGAATTGGTCGTTTGGTCCTCCCAAAAGCATGTGCTGAAgtaagttttcaaaagtagtcatcctcaatacctttgtttcttgtatgctagcttgtgttatttattttgtggaggtttttcttccttttcatatgtataaactgtaagtcaatacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|5268958|gb|AI770922.1|AI770922

EST:     GAGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAG                         GCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAA
genomic: GTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaa ... ctctgtgtagGCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAA

EST: gi|67040653|gb|CO466908.1|CO466908

EST:     GTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAG                         GCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAA
genomic: GTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaa ... ctctgtgtagGCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAA

EST: gi|13150757|gb|BG321079.1|BG321079

EST:     GTSATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAG                         GCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAA
genomic: GTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaa ... ctctgtgtagGCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 100

TTCAAATTCCACAATCGTTCCATTGTTCGAGAAGGTCCTGAGTGCGAGTGATGCAGGCCGCATAGGGCGCCTTGTTCTTCCAAAAGCTTGTGCCGAGgtaactttaa ... ctctgtgtagGCATATTTTCCCCCAATTTCTCAACCAGAAGGACGCCCCTTGACAATTCAAGATGCAAGAGGAAAAGAATGGCATTTTCAGTTTAGGTTTTGGCCAAATA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 89

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 87