ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GAAATTTTGTCAGTGTTCCTTTGAGAGATGAGTTCCTTGAAAATGCTAGGCTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaaatttctctttcagcaatgctttagcaggaggatgatgattgttaagtataagtattgacacattttcagatctctaatttgttttctagt...

Basic information

species	Zea mays
transcript	GRMZM2G151549_T01
intron #	5
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G151549_T01 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA02G33770.1 (Glycine max), 5'ss of exon 6

GAAATTTTGTCAGTGTTCCTTTGAGAGATGAGTTCCTTGAAAATGCTAGGCTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaaatttctctttcagcaatgctttagcaggagg----atgatgattgttaagtataagtattg-acacattttcagatctctaatttgttttctagt
| || |||   | ||| ||  | || |||||||||||||||||||||||||| ||||| || || ||||||||  | || || |   | || || ||  ||| ||   |  |||||  |  |   ||| | ||||    ||    || ||  || | |  |   |  |||   ||| | |  | | | | ||| |    |||   
GCAACTTTTCAACTGTACCACTAAGGGATGAGTTCCTTGAAAATGCTAGGCTATTTATCTTTGAAACATACTGTAGAATACACCAACGGATTGACATGGGgtatgc--acctttctaatataa-gatgttgtagctcttggctttataatttttctgatatgctacaattttgcacctgtgaaaaacac-aatgtagcctctgt-

upper sequence: GRMZM2G151549_T01 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA10G16090.1 (Glycine max), 5'ss of exon 6

GAAATTTTGTCAGTGTTCCTTTGAGAGATGAGTTCCTTGAAAATGCTAGGCTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaaatttctctttcagcaatgctttagcaggaggatgatgattgttaagtataagtat-tgacacattttcagatctct--aatttgttttctagt-
| || |||   | ||| ||  | || |||||||||||||||||||||||||| ||||| ||||||||||||||  | || || |   |||| || ||  ||| ||    |  ||||  |  |  |||| | ||||       |  |      ||  | |    || ||  ||| |||   | || |  ||  ||   | |||  
GCAACTTTTCAACTGTACCGCTAAGGGATGAGTTCCTTGAAAATGCTAGGCTATTTATCTTCGAGACATACTGTAGAATACACCAACGCATTGACATGGGgtatgc---accttctaatataa-aatgttgtagctaagctctctttgcctatagtttttctgatatgctacaattttgcacctgtggaaaatgcaatgtagcc

upper sequence: GRMZM2G151549_T01 (Zea mays), 5'ss of exon 5
lower sequence: Vv00s0252g00070.t01 (Vitis vinifera), 5'ss of exon 6

GAAATTTTGTCAGTGTTCCTTTGAGAGATGAGTTCCTTGAAAATGCTAGGCTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaaatttctctttcagcaatgcttta-gcaggaggatgatgattgttaagtataagtattgacacattttcagatctctaatttgttttctagt--
|||  |||   | ||| || ||||| |||||||| || ||||||||  ||||||||||||| |||||||||||||| || ||||   | || || ||  ||| |    |   | || | | |    || || |   ||    ||  |  ||| || | | |   || |||    ||    | |     ||  | |   |||  
GAAGCTTTTCTACTGTGCCATTGAGGGATGAGTTTCTCGAAAATGCCCGGCTTTTTATTTTTGAGACATACTGCCGAATACATCAACGAATTGACATGGGgtatattta---tactttctgaaatttgattaatatagcttcattctttttgataggcaaattgatagactacattgattagcatagcttcatgtgggagtgc

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|5871211|gb|AW017682.1|AW017682

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACCATGAGCTACGATGAGGCCGAGTTGTGGA
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAA-CATGAGCTACGATGAGGCCGAGTTGTGGA

EST: gi|60354741|gb|DN221714.1|DN221714

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|211499749|gb|FL295237.1|FL295237

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCC
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCC

EST: gi|12968652|gb|BG265598.1|BG265598

EST:     CTTTTTATTTTCGAGACATACTGCGGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATG
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATG

EST: gi|211496877|gb|FL295235.1|FL295235

EST:     CTGTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTTCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|67023707|gb|CO452456.1|CO452456

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|60354489|gb|DN221462.1|DN221462

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|71772603|gb|DR970531.1|DR970531

EST:     CCTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|148929371|gb|EC875323.2|EC875323

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|5915274|gb|AW052915.1|AW052915

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|211221048|gb|FL468686.1|FL468686

EST:     CTGTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTTCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|78113788|gb|DV532177.1|DV532177

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|31406350|gb|CD485082.1|CD485082

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|61119784|gb|DN560745.1|DN560745

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|6020868|gb|AW065796.1|AW065796

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|148988253|gb|EE031627.2|EE031627

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|148947188|gb|EC892526.2|EC892526

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|6056588|gb|AW090993.1|AW090993

EST:     CTTTTTATTTTCGAGACATACTGCCGTATNCATCGGNGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGNGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|33092079|gb|CF052073.1|CF052073

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|6194537|gb|AW146641.1|AW146641

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|211499748|gb|FL295236.1|FL295236

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

EST: gi|29946191|gb|CB816025.1|CB816025

EST:     CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAG                         CATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT
genomic: CTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGAT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 316

GAAATTTTGTCAGTGTTCCTTTGAGAGATGAGTTCCTTGAAAATGCTAGGCTTTTTATTTTCGAGACATACTGCCGTATCCATCGGTGCATCGATATCAGgtgtggcaaa ... caacggacagCATGCTTGCTCAGAAGTTGAACATGAGCTACGATGAGGCCGAGTTGTGGATCATGAATTTGGTTAGAAGCTCAAAACTGGATGCCAGGATCGATTCAGTA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 236