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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TTGGTGCCGATATCTCACTTGATACTGCAACTGGGAACTTGACTAAATACAATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttcaaagtgtagtaatcctatatacttaggcactgtatgtgactgttatttggcagcacttgtaatatcattatttatggttaagtacaaatc...

Basic information

species Zea mays
transcript GRMZM2G150616_T01
intron # 5
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|4804484|gb|AI665354.2|AI665354
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|32937165|gb|CF041984.1|CF041984
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|32840535|gb|CD980216.1|CD980216
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|89252324|gb|DY624110.1|DY624110
EST:     GAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCCCAAGGTGAGGGA
genomic: GAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|18163396|gb|BM333235.1|BM333235
EST:     GTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: GTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|21389982|gb|BQ528031.1|BQ528031
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|166452843|gb|EG124947.1|EG124947
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTAC
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTAC
EST: gi|32937576|gb|CF042395.1|CF042395
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|463336|gb|T18303.1|T18303
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCTTTANCTGGAGCCTACTATCACAAGGTGAGCG
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTA-CTGGAGCCTACTATCACAAGGTGAGCG
EST: gi|44900340|gb|CK826885.1|CK826885
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|22472745|gb|BU037225.1|BU037225
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|22472744|gb|BU037224.1|BU037224
EST:     GTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: GTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|148936370|gb|EC880462.2|EC880462
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|5018349|gb|AI714542.1|AI714542
EST:     GAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: GAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|78026384|gb|DV494771.1|DV494771
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|148936369|gb|EC880461.2|EC880461
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGACCCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|211412252|gb|FL229038.1|FL229038
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|22715017|gb|BU197555.1|BU197555
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|20337022|gb|BQ172652.1|BQ172652
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|149110762|gb|EE190597.2|EE190597
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|60350302|gb|DN217275.1|DN217275
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|148973802|gb|EE024938.2|EE024938
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|116815705|gb|EC858392.1|EC858392
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTG
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTG
EST: gi|211412253|gb|FL229039.1|FL229039
EST:     TGCTGCACTGAGGGTCACTCATGAAGACCTTTGTCGCATCCCCTGAACCT                         GAACAACAAGGGAGACAGCTTTTACTGGAGCCTACTATCACAAGGTGAGCG
genomic: TGCTGCACTGAGGTTCACTCATGAAGACCTT-GTCGCATCCC-TGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTT-ACTGGAGCCTACTATCACAAGGTGAGCG
EST: gi|31348886|gb|CD433243.1|CD433243
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|14204290|gb|BG837967.1|BG837967
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|37379340|gb|CF626412.1|CF626412
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|32937434|gb|CF042253.1|CF042253
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|60354864|gb|DN221837.1|DN221837
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|20802988|gb|BQ294038.1|BQ294038
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|22542179|gb|BU092617.1|BU092617
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|211412250|gb|FL229036.1|FL229036
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGG
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGG
EST: gi|93013107|gb|EB638627.1|EB638627
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|94473288|gb|EB702246.1|EB702246
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|20208147|gb|BQ134236.1|BQ134236
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTGGCATCCCTGAACCT                         GAACAACAAGGGCGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|211412255|gb|FL229041.1|FL229041
EST:     AATGCTGCACTGAGGGTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|211272935|gb|FL017962.1|FL017962
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|60337478|gb|DN204451.1|DN204451
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
EST: gi|148973801|gb|EE024937.2|EE024937
EST:     AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCT                         GAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA
genomic: AATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 419

TTGGTGCCGATATCTCACTTGATACTGCAACTGGGAACTTGACTAAATACAATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGAATTGACCAACACAGCTGTTGGGGCAGAGCTTACCCACAGCTTCTCGACC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 475

TTGGTGCCGATATCTCACTTGATACTGCAACTGGGAACTTGACTAAATACAATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGAATTGACCAACACAGCTGTTGGGGCAGAGCTTACCCACAGCTTCTCGACC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 429

TTGGTGCCGATATCTCACTTGATACTGCAACTGGGAACTTGACTAAATACAATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGAATTGACCAACACAGCTGTTGGGGCAGAGCTTACCCACAGCTTCTCGACC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 311

TTGGTGCCGATATCTCACTTGATACTGCAACTGGGAACTTGACTAAATACAATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttc ... tctcttgcagGAACAACAAGGGAGACAGCCTTACTGGAGCCTACTATCACAAGGTGAGCGAATTGACCAACACAGCTGTTGGGGCAGAGCTTACCCACAGCTTCTCGACC


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TTGGTGCCGATATCTCACTTGATACTGCAACTGGGAACTTGACTAAATACAATGCTGCACTGAGGTTCACTCATGAAGACCTTGTCGCATCCCTGAACCTgtgagttttcaaagtgtagtaatcctatatacttaggcactgtatgtgactgttatttggcagcacttgtaatatcattatttatggttaagtacaaatc

- - - - - - - - - - - - - - - - - - - - - - - - -  ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA