ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

TCCCTGGGATGAAGCAGCGTCCACCACGCGGGTATAATCCCTACCATGGCTACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattgtggtgtactactgtcaatatggatttctagttttctttctaacgtaatcgtttatggttttgcag

Basic information

species	Zea mays
transcript	GRMZM2G108919_T03
intron #	5
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G108919_T06 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os02g52140.2 (Oryza sativa), 5'ss of exon 5

TCCCTGGGATGAAGCAGCGTCCACCACGCGGGTATAATCCCTACCATGGCTACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg-tggtgtactactgtcaatatggatttcta---gttttctttctaacgta---atcgtttatggttttgcag
||||||||||||||||||||||||| || || || ||||| || ||||| ||||| |||||||| ||||| || || || ||||| ||||| ||||| |||||||| | | |  | |   |   |  || |  | ||||    || |  | |||||| |    || || |||  || | |||
TCCCTGGGATGAAGCAGCGTCCACCGCGTGGATACAATCCTTATCATGGTTACCCATATAGATCGTATGGGGCCCCATATTTCCCACCATATGGTTACGGgtaagtttagcttttttgacaacatggatgtctaattctttatgtctggtgtctaacatccatattgtgtattcttgttcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|9731847|gb|BE510599.1|BE510599

EST:     TACCCTTATAGATCATATGGAGCCCCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|9731934|gb|BE510686.1|BE510686

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|6563947|gb|AW231569.1|AW231569

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|7135303|gb|AW498018.1|AW498018

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|50329632|gb|CO524758.1|CO524758

EST:     TACCCTTATAGATCATATGGAGCCCCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGC
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGC

EST: gi|211177618|gb|FL479926.1|FL479926

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|60399607|gb|DN232418.1|DN232418

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|60351908|gb|DN218881.1|DN218881

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|89247954|gb|DY619740.1|DY619740

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTTTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

EST: gi|32859080|gb|CD998761.1|CD998761

EST:     TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGG                         GAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG
genomic: TACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 93

TCCCTGGGATGAAGCAGCGTCCACCACGCGGGTATAATCCCTACCATGGCTACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattg ... ggttttgcagGAGGGCTCCTAGATTCCGCCGCCCTATGCGCTACAGACCTTACTTCTGAAGTACGTGCGGGGTAATAATGTTCAATGCAAAACCAGCCATGGTTAGTGGT

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 69

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 68

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 113

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TCCCTGGGATGAAGCAGCGTCCACCACGCGGGTATAATCCCTACCATGGCTACCCTTATAGATCATATGGAGCACCGTACTTCCCCCCATACGGTTATGGgtaagtattgtggtgtactactgtcaatatggatttctagttttctttctaacgtaatcgtttatggttttgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGATCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCA