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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtggtttctgtttgaacattagcgtaacttcatatctgctagtggccataaccacatgacttttccttccttttgctccag

Basic information

species Zea mays
transcript GRMZM2G079538_T01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G079538_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os04g32330.1 (Oryza sativa), 5'ss of exon 6
AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtggt-------------ttctgtttgaacattagcgtaacttcatatctgctagtgg-ccataaccacatgacttttccttccttttgctccag
||||||| |||||||| ||||||||||| || ||||| |||||||||||||||||||||  |    |             |||||| |||| |     | || ||   |        | | |  | |  |  |  | || |||| || |   |||||
AACCTGGTGACAGAGTTGAGGCTGATGAGCCCATAGCACAGATTGAAACTGATAAGgtacagtcattcaatcactttctgttctgtctgaatagacatgcaatttagcactcatggatagtcttttaaaatgtatcattgcctttctgt-attccag

upper sequence: GRMZM2G079538_T01 (Zea mays), 5'ss of exon 5
lower sequence: AT4G26910.3 (Arabidopsis thaliana), 5'ss of exon 3
AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtg---gtttctgtttgaacattagcgtaacttcatatctgctagtggccataaccacatgacttttccttccttttgctccag
||||||| || |||||| ||||||||||  |||| || || |||||||||||||||||||   |     ||| |  ||   ||||    |||   ||| | || ||      | || |   |   |  | | |  || ||    ||
AACCTGGTGAGAGAGTCCAGGCTGATGAGGCTATTGCACAAATTGAAACTGATAAGgtatcaatatttattttttctttgtcatttttctaaagacatctgtgttaaattttaaaatctattatttgattcatatttgtggattag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|5343075|gb|AI795485.1|AI795485
EST:     GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAG                         GTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
genomic: GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
EST: gi|149074999|gb|EE163468.2|EE163468
EST:     GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAG                         GTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
genomic: GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
EST: gi|32808216|gb|CD960450.1|CD960450
EST:     GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAG                         GTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
genomic: GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
EST: gi|7145229|gb|AW506710.1|AW506710
EST:     GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAG                         GTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
genomic: GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG
EST: gi|29576970|gb|CB617082.1|CB617082
EST:     GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAG                         GTCACTATAGATGTCGCAAGTCCTGAGGCTGGAGTTATTGAAAAG
genomic: GAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 270

AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 313

AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 294

AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 292

AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtgg ... tttgctccagGTCACTATAGATGTCGCAAGTCCTGAGGCTGGTGTTATTGAAAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AACCTGGAGACAGAGTCGAGGCTGATGAACCTATAGCGCAGATTGAAACTGATAAGgtattggtggtttctgtttgaacattagcgtaacttcatatctgctagtggccataaccacatgacttttccttccttttgctccag

- - - - - - - - - - GCTGAT
- - - - - - - - - - - - - - - - - -  GCAGAT