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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagattactgttaatacttattctccctcattgggtattgggcattcacaagattattttgattcaagctagtgtaaggttaaaacaatgctgaat...

Basic information

species Zea mays
transcript GRMZM2G031824_T01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G031824_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os09g22440.1 (Oryza sativa), 5'ss of exon 5
GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgt-tgtagattactgttaatacttattctccctcattgggtattgggcattcacaagattattttgattcaagctagtgtaaggttaaaacaatgc--tgaat--
|| ||||| |||||||||||||||||||| || || ||  ||||||||||||||||||||||||||||||| |||||||||||||| |||||||| |||  |  ||| |    | || |  |   |  |  | | |     | |   |  |   || ||  | || ||| | |  |    ||   || |  ||  |  
GTGATGGCTGTTATGTCAATGTGGACTAGGTTACAACCGCTTTATGCTGCAAACATGTGGAATGAAGCCCTTAACAAACGACTTGGGACTGAGgtctgtgaacaactctgtgca-ttgtaatggtt--tcagctttataaaaattggactacagttctg--tgaaactactctttgccacaattcatactttgggtac

upper sequence: GRMZM2G031824_T01 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA01G04400.1 (Glycine max), 5'ss of exon 5
GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagattactgttaatacttattctccctcattgggt-attgggcattcacaagattattttgattc-------aagctagtgtaaggttaaaacaatgctgaat
|||||  | || ||||| |||||||| ||| ||||||||| ||||||||||||||||||||||||||| || ||||| ||  | || ||||||||  ||  ||  ||||  |    | |     |  ||   | | |   |  ||  | || |  ||| | ||           || |  | ||| |||||||        
GTAATTTCTGTCATGTCTATGTGGACAAGAATGCAGCCAGCTTATGCTGCAAACATGTGGAATGAAGCGCTGAACAAGCGGTTAGGGACTGAGgt-atatcttgatgttggtctacagtagatgtgcttacatgaatatactgtcctatgacttcttttactctttatgtgtaatatttcacggtcaaaacaag-------

upper sequence: GRMZM2G031824_T01 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA02G03160.1 (Glycine max), 5'ss of exon 5
GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagattactgttaatacttattctccctcattgggt-attgggcattcacaagattattttga-ttcaagctagtgtaaggttaaaacaatgctgaat
|||||  | || ||||| |||||||| ||| ||||||||| ||||||||||||||||||||||||||| || ||||| ||  | || ||||||||  ||  ||  ||||  |    | |   | |  ||   | | |      ||  | ||||  ||| | ||       ||   |  | | |   | ||  |  
GTAATTTCTGTCATGTCTATGTGGACAAGAATGCAGCCAGCTTATGCTGCAAACATGTGGAATGAAGCACTGAACAAGCGGTTAGGGACTGAGgt-atatcttgatgttggtctacaatagacatgcttacatgaatatattgtcctatgatttcttttactttttatgggtaacatttcacagtcaagccaag-

upper sequence: GRMZM2G031824_T01 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA18G17350.1 (Glycine max), 5'ss of exon 4
-GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagattactgttaatacttattctccctcattgggtattgggcattcacaagattattttgattcaagctagtgtaaggttaaaacaatgctgaat------
 |||||  | || ||||| |||||||||||||||||||||| |||| | ||||| ||||||||||||||  | || || ||  | || ||||||   |||  |    |       |||  |       | ||       | || ||     ||  | | | |  | |||||||    |        | ||   |      
GGTAATTTCTGTCATGTCTATGTGGACTAGATTGCAGCCAGCTTATTCGGCAAATATGTGGAATGAAGCATTGAATAAGCGGTTAGGGACTGAg---gtaagtcttggcactggtttaaacatttctgtgggaagaatagtatccat----ttgctctaaatgtatctagtgttctttccttcttttcctttttccctaa

upper sequence: GRMZM2G031824_T01 (Zea mays), 5'ss of exon 5
lower sequence: Vv01s0026g00920.t01 (Vitis vinifera), 5'ss of exon 5
GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagattactg---ttaatacttattctccctcattgggtattgggcattcacaagattattttgattcaagctagtgtaaggttaaaacaatgctgaat-
|| ||  | || ||||| |||||||||||| |||||||||  ||||||| ||| |||||||||||||| ||||| ||  | ||||  || |||||  | |  | |||   | |||  ||        | |||  |||||    |||   || || || |   || || |     |    ||     |||| |   | 
GTCATTTCTGTCATGTCTATGTGGACTAGAATGCAGCCAGCATATGCTGAAAATATGTGGAATGAAGCTCTAAATAAGAGGCTTGAGACCGAGgtatttgtgttctgaattcaatgtttcagtggattaattcagtattaa-tattgttaaaat-atatatcttaaaac--acttggattttctctaatgttatctt

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32842172|gb|CD981853.1|CD981853
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32925672|gb|CF030484.1|CF030484
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32850032|gb|CD989713.1|CD989713
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841464|gb|CD981145.1|CD981145
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841170|gb|CD980851.1|CD980851
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32924871|gb|CF029683.1|CF029683
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32924893|gb|CF029705.1|CF029705
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841167|gb|CD980848.1|CD980848
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|78113166|gb|DV531560.1|DV531560
EST:     AAGCCCTAAACAAACGACTTGGCACTGAG                         GATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: AAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|78111912|gb|DV530308.1|DV530308
EST:     ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAG                         GATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32925223|gb|CF030035.1|CF030035
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGCCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841238|gb|CD980919.1|CD980919
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32921666|gb|CF026478.1|CF026478
EST:     ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAG                         GATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTAGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32924738|gb|CF029550.1|CF029550
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841288|gb|CD980969.1|CD980969
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|88748743|gb|DY532884.1|DY532884
EST:     GCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGA-                         GATTTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTC
genomic: GCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGA-TTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTC
EST: gi|32925872|gb|CF030684.1|CF030684
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32924755|gb|CF029567.1|CF029567
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAATGACGTGGAATGTC
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAA-GACGTGGAATGTC
EST: gi|32839582|gb|CD979263.1|CD979263
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32840853|gb|CD980534.1|CD980534
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32835862|gb|CD975540.1|CD975540
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32925065|gb|CF029877.1|CF029877
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32922055|gb|CF026867.1|CF026867
EST:     ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAG                         GATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTAGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32920261|gb|CF025073.1|CF025073
EST:     ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAG                         GATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTAGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841249|gb|CD980930.1|CD980930
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32840052|gb|CD979733.1|CD979733
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACGTGGCACTAAG                         GAGTTGGCCCTTCAGGGGATCGTTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
EST: gi|32841608|gb|CD981289.1|CD981289
EST:     ATGCTGCAAACATGTGGAATGAAGCTCTAAACAAACGACTTGGCACTGAG                         GATTTGGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT
genomic: ATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 171

GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCTTTTGATCAGTTGCTCACCATACCCGAGCAAGACGAATGGGTGTACAGTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 275

GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCTTTTGATCAGTTGCTCACCATACCCGAGCAAGACGAATGGGTGTACAGTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 396

GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCTTTTGATCAGTTGCTCACCATACCCGAGCAAGACGAATGGGTGTACAGTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 302

GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagatt ... tcttgtatagGATTTCGACCTTCAGGGGATCATTATGGAGATTGAAAGACGTGGAATGTCTTTTGATCAGTTGCTCACCATACCCGAGCAAGACGAATGGGTGTACAGTG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTAATGGCAGTTATGTCAATGTGGACTAGATTGCAGCCAGTTTATGCTGCAAACATGTGGAATGAAGCCCTAAACAAACGACTTGGCACTGAGgttgtagattactgttaatacttattctccctcattgggtattgggcattcacaagattattttgattcaagctagtgtaaggttaaaacaatgctgaat

- - - - - - - - - - - - - - - - - - - - -  ATGCTG
- - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tctccctc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - taaaacaa