ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttctttttctcccatttcaaccttcgagatttaccacctgctagcattatcatttcatccatcttcatcaaaaaccag

Basic information

species	Zea mays
transcript	GRMZM2G416061_T01
intron #	4
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os02g37430.1 (Oryza sativa), 5'ss of exon 3

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttctttttctcccatttcaaccttcgagatttaccacctgctagcattatcatttcatcca-tcttcatcaaaaaccag
||||||||||| |||||||| || |||||||||||| ||||||||||||| |||||||||||||| ||||| ||||||||| |||||||||| ||| | |||  ||    | |||   |  | | |    | ||  ||    | |||||    |  |  | | ||| |  ||| |||   || |||
GGAAACAACATTCGCTATTACATTCTTCCTGACAGCTTAAACCTGGAGACATTGCTGGTTGAGGATACACCGAGGGTCAAGGCTAAGAAGCCAACTGCAGgtttgataattgttttgtctctctaag---ttgattttactgagctgctgaactgtttgtctgatcaaaccttaatccggaaacag

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA02G10640.1 (Glycine max), 5'ss of exon 4

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgt------cagagttct---------ttttctcccat-ttcaaccttcgagatttaccacctgctagc--------attatcatttcatc-catcttcatcaaaaa-ccag
|| ||||||||||| || ||||| |||||||||||| | || || ||||| |||||||| || || ||||| | | |||| || |||||||| ||| |||||       | ||||           |||||  ||||  ||||    |   | ||    | || |            || | |||| | |  || ||||| | |   ||||
GGTAACAACATCCGTTACTATATTCTTCCTGACAGCTTGAATCTTGAGACCTTGCTGGTGGAAGAGACACCCAAGATCAAACCCAAGAAGCCAACTGCTGgtactgtttatagtttgactacaaaattttcatccatactcaattcactctaattgtttcttggtgagttccccaggttttaattttagcatattttcattacatttccag

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA19G33700.1 (Glycine max), 5'ss of exon 3

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttctttttctcccatttcaaccttcgagatttaccacctgctagc-attatcatttcatccatcttcatcaaaaaccag----------------------------
|| |||||||| || ||||||||||||||||||||| | || || |||||||| |||||||| ||  | || | | |||||||||||||||| ||| |||||   | |   | || |   | ||  || |   |  |||| ||   || |   ||  |||  |   |  |  ||    || | | |                            
GGCAACAACATTCGATATTATATCCTTCCTGACAGCTTGAATCTTGAGACTTTACTGGTTGAAGAGGCTCCTAAGATCAAGCCTAAGAAGCCAACTGCTGgt---attgtatattatttgactttcac-tagaatgtttatcagaggccaattatgttcaggttgactgttatctaggaatatctggttctcaaagttttgattttatttacag

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA03G30880.1 (Glycine max), 5'ss of exon 3

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcaga---gttctttttctcccatttcaaccttc----gagatttaccacctgctagc-----attatcatttcatccatcttcatcaaaaaccag-------------
|| |||||||| || ||||||||||||||||||||| | || || |||||||| |||||||| ||  | || | | |||||||||||||||| ||| |||||        || |||  ||  || |  ||  ||     ||  || |  |  | |  |       ||||||    ||   |  |  |||||     |             
GGCAACAACATTCGATATTATATCCTTCCTGACAGCTTGAATCTTGAGACTTTACTGGTTGAAGAGGCTCCTAAGATCAAGCCTAAGAAGCCAACTGCTGgtattgtatattatttgactgtcactaaaatgtttatcagaagttaattatgttcaggttgactgttatcaagggatatctggttctcaaagttttgattttatttgcag

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: AT4G02840.2 (Arabidopsis thaliana), 5'ss of exon 4

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagag----ttctttttctcccatttcaaccttcga----gatttaccacctgct-agcattatcatttcatccatcttcatcaaaaaccag
||||||||||| ||||||||||| ||||| |||||  | || |||||||||||||||||||| || || |||||  |||| || || ||||| ||| | ||| | |     ||  |||| ||  |  |   |||| |     | |||      |||| ||  | ||| ||  ||   |||   |     | |||
GGAAACAACATTCGCTATTATATTCTTCCGGACAGTTTGAATCTGGAGACTTTGCTGGTTGAAGACACTCCAAGAATCAAACCCAAAAAGCCAACTGCAGgtaacaaaacatttcttttttctgaactatgcctttgtttttgtttttgtttttgcttagactcatctttctattgttctcttt--ggtatcag

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: Vv07s0104g00160.t01 (Vitis vinifera), 5'ss of exon 5

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttctttttctcccatttcaaccttcgagatttaccacctgctagcattatcatttcatccatcttcatcaaaaaccag-----------
|| |||||||| || |||||||||||||| |||||| | || || |||||  |  |||| || || ||||| ||||||||||| |||||||| ||  | ||| |  | ||| | |||     |  ||   |    |||| |       |    ||  | ||   |  || ||  | ||                  
GGCAACAACATTCGGTATTATATCCTTCCCGACAGCTTGAATCTTGAGACCCTTTTGGTGGAAGAGACACCTAGGGTCAAGCCCAAGAAGCCAACCGCAGgt-attgctctatctctttggctctaattctatgcattttcgtatgatttatgttccccttgttttaattttttttaatgcttttttccatttcag

upper sequence: GRMZM2G416061_T01 (Zea mays), 5'ss of exon 4
lower sequence: Vv19s0015g01330.t01 (Vitis vinifera), 5'ss of exon 3

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgt----cagagttctttttctcccatttcaaccttcgagat-ttaccacctgctagcattatcatttcatccatcttcatcaaaaaccag
|| ||||| || || |||||||| ||||| |||||| |||| |||||||| |||||||| || || ||||| ||||||||||| |||||||| ||| |||||    || | |||  |||   |   |  | | |  || |  |||  || |  ||  ||| |  ||| || | |||  |    || ||||
GGCAACAATATTCGGTATTATATACTTCCCGACAGCATAAATCTGGAGACATTGCTGGTGGAAGAGACACCTAGGGTCAAGCCCAAGAAGCCAACTGCTGgtattgcatatttcccttttgacttatataccttgtgatagattaatactttttaaaatttttttttaatgcctctata----aatccag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|32828991|gb|CD968669.1|CD968669

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTG                         GGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGCGCGCGGTCGGGGTC
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC

EST: gi|211405279|gb|FL027503.1|FL027503

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTG                         GGAAGCCCTTGGGACGTGGGCGTGGCCGTGGTCGTGGGCGCG
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCG

EST: gi|211405278|gb|FL027502.1|FL027502

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTG                         GGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC

EST: gi|67011942|gb|CO440691.1|CO440691

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTG                         GGAAGCCCTTGGGACGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC

EST: gi|26557688|gb|CA829923.1|CA829923

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTG                         GGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC

EST: gi|211405280|gb|FL027504.1|FL027504

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAARAAGCCTACTTCTG                         GGAAGCCCTTGGGCCGTGGGCGTG
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTG

EST: gi|32847843|gb|CD987524.1|CD987524

EST:     TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTG                         GGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC
genomic: TTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 96

GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttc ... caaaaaccagGGAAGCCCTTGGGCCGTGGGCGTGGCCGTGGTCGTGGGCGCGGTCGGGGTCGGGGACGTTGAGCATGCTAGCTGCTTTTGGAAATTGCATTGCGTGCATC

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 19

Block sizes: 43 (upstream exon), 49 (downstream exon)
Score: 21

Block sizes: 38 (upstream exon), 49 (downstream exon)
Score: 26

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GGAAACAACATCCGCTATTATATCCTTCCTGACAGCCTAAACCTGGAGACTTTGCTGGTTGAGGAAACACCAAGGGTCAAGCCTAAGAAGCCTACTTCTGgtcagagttctttttctcccatttcaaccttcgagatttaccacctgctagcattatcatttcatccatcttcatcaaaaaccag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAAGC