ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtctgtcatttcctgttgccatccagcctgaattcttgctgataacattgtgtctgcag

Basic information

species	Zea mays
transcript	GRMZM2G172001_T04
intron #	4
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G172001_T04 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os01g66420.2 (Oryza sativa), 5'ss of exon 4

GCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtctgt-cat---ttcctgttgccatccagcctgaattcttgctgataaca--ttgtgtctgcag---
||| || ||||||||||||||||||||||| || |||||||||||||| || ||||| || || ||||| ||||||||||||| ||||||||| ||||||||||||   ||  |||    | |||||         | |||||   ||||||  |||  || | |||||||   
GCCAACAATATTTGAGGTAGTGACAGGAGCCGCTAAGAAGCAGGCGAAAGAAAAGACTCCCAACAGTAGTAGCAAGAGCAACAAGCCTAGTTCGAAAGTGgtaaattaatgagcatgcacttctgttttatcgtgacttgaatctctgctgagtacaatttctttctgcagcag

upper sequence: GRMZM2G172001_T04 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os01g66420.1 (Oryza sativa), 5'ss of exon 4

GCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtctgt-cat---ttcctgttgccatccagcctgaattcttgctgataaca--ttgtgtctgcag
||| || ||||||||||||||||||||||| || |||||||||||||| || ||||| || || ||||| ||||||||||||| ||||||||| ||||||||||||   ||  |||    | |||||         | |||||   ||||||  |||  || | |||||||
GCCAACAATATTTGAGGTAGTGACAGGAGCCGCTAAGAAGCAGGCGAAAGAAAAGACTCCCAACAGTAGTAGCAAGAGCAACAAGCCTAGTTCGAAAGTGgtaaattaatgagcatgcacttctgttttatcgtgacttgaatctctgctgagtacaatttctttctgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|74237049|gb|DT644963.1|DT644963

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTG                         CAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|211166632|gb|FK973167.1|FK973167

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTG                         CAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|60352400|gb|DN219373.1|DN219373

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGT-                         --GTCAAGAGCAGAGTCTCGTTCAAAGGTTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|78023803|gb|DV492190.1|DV492190

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCNTAGTTCAAAAGTG                         CAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|149042112|gb|EE047090.2|EE047090

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTG                         CAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|60397867|gb|DN230683.1|DN230683

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGT-                         --GTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|71756745|gb|DR954682.1|DR954682

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGT-                         --GTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|5456317|gb|AI834007.1|AI834007

EST:     AGAAGNCNCCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTG                         CAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

EST: gi|78110539|gb|DV528949.1|DV528949

EST:     AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTG                         CAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA
genomic: AGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 139

GCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtct ... gtgtctgcagCAGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAAGAGAGCGGTGATGACGATGAGGATGAGGAGGCAGACGAGCACAACAATA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 29

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 43

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 45

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGgtaaatgtctgtcatttcctgttgccatccagcctgaattcttgctgataacattgtgtctgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAGCA