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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaattaacttgctgaatgtatggtctataaataccaaaggacttatagactaacttggctttatccccag

Basic information

species Zea mays
transcript GRMZM2G167886_T02
intron # 4
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G167886_T02 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os09g12550.1 (Oryza sativa), 5'ss of exon 4
TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaattaacttgctgaatgtatggtctataaataccaaaggacttatagactaacttggctttatccccag
||||| |||||  | |||  ||||| | ||||  |||| || | | | |||| |||||||||||||| ||| | |||||||||  ||||||||| |||||||| | |||   |  |||||||| |||| |||   ||    |||  |||||  ||||||||   |||| || | ||
TGCATCGGTGGTCTGCCAGGTATTGTCTTCTCCTGCCTGCTCCAAGGTCAACCATTGCAGCTGCATTCAGTCCGGATGGGAGAACACTTGCATCCACACAgtaagctaaaggatatgctgaatatatgttctg--aacggaaaaaaactta--gactaactacacttt-tctctag

upper sequence: GRMZM2G167886_T02 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA19G35370.3 (Glycine max), 5'ss of exon 3
TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaattaacttgctgaatgtatggtctataaat---accaaaggacttata-gactaa--cttg----gctttatccccag
|||     | |  | | || ||||| ||| |   |||| || | | | | ||||||||||| ||||||||  ||||||||| ||| ||||| || |||||||| |||  |||||| | |||     |  |   ||| |   | | | | |||| |  || | |  ||||    |  ||||   |||
TGCGGCATTTGTCTGCTAAGTATTGTCCTTTGGTGCCTCCTCCAAGGTCTACTATTGCAGCAGCATTTAGCCCAGATGGGAAAGTTCTTGCCTCCACACAgtaagtt--ttaactagttgatctcctctttactaatttgaatctatgaacttttgtgattgaatcttgttatgtgttatgtgcag

upper sequence: GRMZM2G167886_T02 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA03G32620.2 (Glycine max), 5'ss of exon 3
TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaattaacttgctgaatgtatggtctataaat---accaaaggactt-atagactaacttggctttatccccag-----
|||     | |  | | || ||||| ||| |   |||| || | | | | ||||||||||| ||||||||  ||||||||| ||| ||||| || |||||||| |||  |||||| | |||     |  |   ||| |   | | | | ||||  |   |  |  | | | | | |   |     
TGCGGCATTTGTCTGCTAAGTATTGTCCTTTGTTGCCTCCTCCAAGGTCTACTATTGCAGCAGCATTTAGCCCAGATGGGAAAGTTCTTGCCTCCACACAgtaagtt--ttaactagttgatctcctctttactaatttgaatctatgaactttgtgatcggatcttgttatgtgctatgtgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|18384883|gb|BM418082.1|BM418082
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         TGGTGACCATACTGCCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
EST: gi|18385319|gb|BM418518.1|BM418518
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         TGGTGACCATACTGTCCAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
EST: gi|18384882|gb|BM418081.1|BM418081
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         TGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
EST: gi|76015702|gb|DT942872.1|DT942872
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         G
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccag-
EST: gi|76015702|gb|DT942872.1|DT942872
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         G
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccag-
EST: gi|22489334|gb|BU049257.1|BU049257
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         TGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
EST: gi|87298785|gb|DY470788.1|DY470788
EST:     ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACA                         TGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA
genomic: ACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 14 (upstream exon), 44 (downstream exon)
Score: 13

TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAATGTACTGCTTGGACATCACCGCACTCCATGGGTG


Block sizes: 36 (upstream exon), 41 (downstream exon)
Score: 13

TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAATGTACTGCTTGGACATCACCGCACTCCATGGGTG


Block sizes: 45 (upstream exon), 48 (downstream exon)
Score: 12

TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAATGTACTGCTTGGACATCACCGCACTCCATGGGTG


Block sizes: 34 (upstream exon), 49 (downstream exon)
Score: 16

TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaat ... ttatccccagTGGTGACCATACTGTCAAAATAATTGATTGGCAAACTGGGAAATGCTTGAATGTACTGCTTGGACATCACCGCACTCCATGGGTG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TGCATTGGTGGATTCCCAAATATTGCCCTCTCACGCCTTCTTCGAAGGCAACTATTGCAGCTGCATTTAGTACAGATGGGAGAGTACTTGCATCTACACAgtaggttaattaacttgctgaatgtatggtctataaataccaaaggacttatagactaacttggctttatccccag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGATG