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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggatccaacacagagaccacggcgcaaaaattttagtagaaagtagaaactatagcgtaccttgtgtatggcctccagtttgttgatttctctgttaaacctagcctctgctgcgtatgtag

Basic information

species Zea mays
transcript GRMZM2G124918_T01
intron # 4
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G124918_T01 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os03g38590.1 (Oryza sativa), 5'ss of exon 4
GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactg------gatccaacacagagaccacggcgcaaa--------aattttagtagaaagtagaaactatagcgtaccttgtgtatggcctccagtttgttgatttctctgttaaacctagcctctgctgcgtatgtag
|| | ||| |||||||||||||| ||||| || ||||| |||||| | |||||||| |||||||||||        ||| ||  |||| |    | ||    |        ||||| | |       |   |   | ||  |  |  |||           |||| | || | |    |     | | | ||   |  | | |||
GATATGAAAACAAGGGAGAAGGAACTTCTTGCAAAGGAAGCCGAGCTAAACAGGCGAGAGAAGgtaacctgaacatgatgcagaacagtgttagctgcatgtacttccttgaatttcaattctttccaactatgttggcaaagttgttgtgccaagatggatttgattatgtgtaacatgctgattgtcgcttacgtctgtatag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|95107983|gb|EB740212.1|EB740212
EST:     AGGCGTGAGAAG                         GAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG
genomic: AGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG
EST: gi|71766083|gb|DR964020.1|DR964020
EST:     GGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAG                         GAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG
genomic: GGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG
EST: gi|211468481|gb|FL364661.1|FL364661
EST:     GGGAGAAGGAGCTTCTGGCCAAGGAGGCCGARTTGAACAGGCGTGAGAAG                         GAAATAAAAAGG
genomic: GGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGG
EST: gi|78111675|gb|DV530072.1|DV530072
EST:     GGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAG                         GAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG
genomic: GGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 41 (upstream exon), 46 (downstream exon)
Score: 18

GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 136

GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 126

GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG


Block sizes: 46 (upstream exon), 46 (downstream exon)
Score: 35

GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggat ... gcgtatgtagGAAATAAAAAGGCGAGAAGACGCCGCAGCACGAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GACAGGAAGACAAGGGAGAAGGAGCTTCTGGCCAAGGAGGCCGAGTTGAACAGGCGTGAGAAGgtaactggatccaacacagagaccacggcgcaaaaattttagtagaaagtagaaactatagcgtaccttgtgtatggcctccagtttgttgatttctctgttaaacctagcctctgctgcgtatgtag

- - - - - - - GGAGAA
- - - - - - - - - AAGGAG
- - - - - - - - - - GGAGCT
- - - - - - - - - -  GAGCTT
- - - - - - - - - - - - - - - -  AAGGAG