ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TTCGGGCTAGCGAAGGCTGGACCTGGAGAAGACGAGACCCACGTGTCGACCCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAGgtatgtacttaggcaaaaaatattatggaacatgcatgcgcgcactcagcagtgacgatctactgttaattttttctgcgttaaaattagcag

Basic information

species	Zea mays
transcript	GRMZM2G110968_T01
intron #	4
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G110968_T01 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os10g25550.1 (Oryza sativa), 5'ss of exon 4

TTCGGGCTAGCGAAGGCTGGACCTGGAGAAGACGAGACCCACGTGTCGACCCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAGgtat----gtacttaggcaaaaaatattatggaacatgcatgcgcgcactc-agcagtgacga--tctactgtta---attttttctgcgtta-aaattagcag------------------------------------------------------------
||||| || || || | ||| |||  |||||||||||| || || || ||  |||| |||||||| |||||||| |||||||| || ||||||||||| ||||      |  | |  || | | | |        |   |     | |  | || |||| |||  | ||   | |   |  |||  ||      |||  |  |                                                             
TTCGGACTGGCCAAAGATGGGCCTCAAGAAGACGAGACACATGTCTCCACTAGAGTGATGGGCACACAGGGCTATGCCGCACCAGAATACATCATGACTGgtactaaaattgtcacacatatagtctacctagctagctagttttgtaaacgagtagtggcgaagtttaacataatatacctttcatgtaccctaaagaaataaaaatatatacctttcatgtttcaactaattggctattaatattacttcatgttattgcag

upper sequence: GRMZM2G110968_T01 (Zea mays), 5'ss of exon 4
lower sequence: Vv04s0008g02070.t01 (Vitis vinifera), 5'ss of exon 4

TTCGGGCTAGCGAAGGCTGGACCTGGAGAAGACGAGACCCACGTGTCGACCCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAGgtatgtacttaggcaaaaaatattatggaacatgcatgcgcgcactcagcagtgacgatctactgttaattttttctgcgttaaaattagcag
||||||||||||||||  || || | ||  |||||||| ||||| || |||||| | |||||||| || ||||| ||||| || ||||| |||||||| ||||  |  ||   ||||| |  |||   ||  | | || |   ||  | |    |  ||||| |  |  | |  |     | | | ||  |||
TTCGGGCTAGCGAAGGACGGTCCAGAAGGCGACGAGACTCACGTATCAACCCGAATAATGGGCACACATGGCTATGCCGCTCCTGAGTATATCATGACTGgtaaat-tttgatcaaaacac-ttagaaaatctcc-tgag---acctaaccccaaaaatctagtact--tctaatacttttaatacttctcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|78089532|gb|DV517906.1|DV517906

EST:     CCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAG                         GGCATCTAACGACCAAGAGCGACGTGTACAGCTTCGGCGTCGTGCTCCTGG
genomic: CCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAGgtatgtactt ... aaattagcagGGCATCTAACGACCAAGAGCGACGTGTACAGCTTCGGCGTCGTGCTCCTGG

EST: gi|67019199|gb|CO447948.1|CO447948

EST:     CCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAG                         GGCATCTAACGACCAAGAGCGACGTGTACAGCTTCGGCGTCGTGCTCCTGG
genomic: CCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAGgtatgtactt ... aaattagcagGGCATCTAACGACCAAGAGCGACGTGTACAGCTTCGGCGTCGTGCTCCTGG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 41 (upstream exon), 14 (downstream exon)
Score: 2

TTCGGGCTAGCGAAGGCTGGACCTGGAGAAGACGAGACCCACGTGTCGACCCGAGTCATGGGCACGCAGGGCTACGCCGCACCCGAGTACATCATGACAGgtatgtactt ... aaattagcagGGCATCTAACGACCAAGAGCGACGTGTACAGCTTCGGCGTCGTGCTCCTGGAGCTGCTAACGGGGCGGAAGGCGTTGGACAAGAACCGGCCGCCGCGAGA

Block sizes: 44 (upstream exon), 39 (downstream exon)
Score: 14

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 29