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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AAACATCCTTGGCGAAGATCTGGGCCCACTCAGCATGAAGGAGCTCGAGCAGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacctggccgcgtttatgtacttatatttccacagagcgcagtgctttcgcttgcatttgacgacggctgatatgctcgcag

Basic information

species Zea mays
transcript GRMZM2G102161_T01
intron # 4
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G102161_T01 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os03g11614.1 (Oryza sativa), 5'ss of exon 4
AAACATCCTTGGCGAAGATCTGGGCCCACTCAGCATGAAGGAGCTCGAGCAGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaa-cacctg---gccgcgtt---tatgtacttatatttccacagagcgcagtgct-ttcgcttgcatttgacgacggctgatatgctcgcag-----
||| || ||||| || ||| |||||||||| |||||||||||||| |||||||| |||||||||||||| |||||||| || || |||||||||||||||||| ||     ||   |||  |||   |||| |   | ||  | ||     |   |||| |||     || |  ||   |  |    |  |          
AAATATTCTTGGTGAGGATTTGGGCCCACTAAGCATGAAGGAGCTGGAGCAGCTTGAGAACCAGATAGAAGTATCCCTCAAACAAATCAGGTCAAGAAAGgtaaaaatgtttgttcgccatgttacctatgcaagcaaatggc-actttttgatatgctcttcttaaacactgaacctagattagattaataatgagctta
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32837918|gb|CD977596.1|CD977596
EST:     AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|32847423|gb|CD987104.1|CD987104
EST:     GCTGGAGAACCAGATAGAGGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: GCTGGAGAACCAGATAGAGG-TATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|67011315|gb|CO440064.1|CO440064
EST:     CAGATAGAGGTTTCCCTNG-AGCATATCAGGGTCAAGGACCA-                         AATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
genomic: CAGATAGAGGTATCCCT-GAAGCATATCA-GGTCAA-GA-AAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|32847744|gb|CD987425.1|CD987425
EST:     AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|32845819|gb|CD985500.1|CD985500
EST:     AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|16917809|gb|BM073443.1|BM073443
EST:     GAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAA                         AATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
genomic: GAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|211408737|gb|FL193400.1|FL193400
EST:     AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|32866292|gb|CF005974.1|CF005974
EST:     AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG
EST: gi|32847872|gb|CD987553.1|CD987553
EST:     AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAG                         AATCAGATGCTACTCGATCAGCTCTTTGATCTCAAAAGCAAG
genomic: AGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 2 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 231

AAACATCCTTGGCGAAGATCTGGGCCCACTCAGCATGAAGGAGCTCGAGCAGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 80

AAACATCCTTGGCGAAGATCTGGGCCCACTCAGCATGAAGGAGCTCGAGCAGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacc ... atgctcgcagAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAG




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AAACATCCTTGGCGAAGATCTGGGCCCACTCAGCATGAAGGAGCTCGAGCAGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGgtagaacacctggccgcgtttatgtacttatatttccacagagcgcagtgctttcgcttgcatttgacgacggctgatatgctcgcag

- - - - - - - - - - - - - - - - - - - - - - - - -  GCTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA