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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatatttctgttctgaagtgagactttttgatatttctattataaactggtcattttgtagtcaaacttttctcccgacctgttactt...

Basic information

species Zea mays
transcript GRMZM2G081653_T01
intron # 4
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os01g23680.3 (Oryza sativa), 5'ss of exon 3
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatatttctgttctgaagtgag-actttttgatatttctattataaactggtcattttgtagtcaaacttttctcccgacctgttactt
  |||| |||||||| |||||||||||||||||||| |||||||||||||||||||||||||||||||| ||||||||||| ||||| || || |||||||||||| | ||||||||| | || | ||| |||  | || ||     ||| ||| | | |      ||||       | || | |    |||| ||| || 
CCGGGGTGCTTTAAGGGCTGAAAAGCCAGAGTTGCTCACTGTAATTTTACCTCAAAGTCTAAAGAAGCAACCTCCTGAAAGTCAAGAGCTTTTATCTAAAgtaagatttcatgatattccagtcccgaattgatcatttcttacctgttccatttttatcaaaatcttttactagagagctctacatg-gaccagtttctc

upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA17G00330.1 (Glycine max), 5'ss of exon 4
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatatttctgttctgaagtgagactttttgatatttctattataaac-----tggtcattttgtagtcaaacttttctcccgacctgttactt-
| | || |||||||| || || || |||||||||||||| || || |||||||||||| |||  || ||||||||||| || || ||||| ||||| ||||||||  |  |   | ||| | |       |  |  || |  |   ||  | |||||       |   | ||||| |   |   |||||    |   |     || 
TCGAGGTGCTTTAAGGGCAGAGAAACCAGAGTTGCTTACAGTTATATTACCTCAAAGTTTAAGCAAACAGCCTCCTGAGAGTCAGGAACTATTGTCCAAAgtaaggtttaaaagtttttatat------atatgctttctcaaaggtttgaatataagtgtttatatactttttgaaaaaaggtttttcatttgctttacatattg

upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA07G40350.2 (Glycine max), 5'ss of exon 4
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcca---tgatatttctgt-tctgaagtgagactttttgatatttctatt-ataaactggtcattttgtagtcaaacttttctcccgacctgttactt
  | || |||||||| || || || || |||||||||||||| || |||||||||||| |||  || |||||||| || || || || || ||||| ||||||||  |  |   |||  ||| | | |      | | |  ||| |||||   | || ||| |||     ||||| |   |   |||||        |     ||
CCGAGGTGCTTTAAGGGCAGAGAAACCGGAGTTGCTTACTGTTATATTACCTCAAAGTTTAAGAAAACAGCCTCCAGAGAGTCAGGAGCTATTGTCCAAAgtaaggtttaagtttgaagtttttatatgctttcttaaaggtttggatataagtgtttatatact-----ttttgaaaaaaggattttcattttctttacatatt

upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: AT2G43945.1 (Arabidopsis thaliana), 5'ss of exon 4
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatatttctgttctgaagtgagactttttgatatttctattataaactggtcattttgtagtcaaacttttctcccgacctgttactt----
 || || || || ||||| || |  |||||| ||||||| |||||| |||| |||||| | || || || |||||||| ||||||||||| ||||||||||||||    ||  |  |   |||||  |||| ||| | || | |   ||| | ||  | |   ||| |   | ||     | | |  | |      | ||    
CAGAGGTGCATTGAGAGCAGAGAGACCAGAGCTGCTTACAGTAATTCTACCCCAAAGTTTGAAAAAACAACCTCCTGAGAGCCAAGAACTATTGTCTAAAgtaag----caaaaactgcatgttcaaaagtcagaatctt-gttgcgtctctgatttatta--catctgaaactcttgtctgtattgctatggtcaatttacag

upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: AT3G59870.1 (Arabidopsis thaliana), 5'ss of exon 4
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaa------tccatgatatttctgttctgaagtgagactttttgatatttctattataaactggtcattttgtagtcaaact---tttctcccgacct--gttactt---------------------------------------
 || || ||  | ||||| || |  |||||| | ||||| || || |||||||||||| | || ||||| |||||||||||||| |||||  |||| |||||||||       | || ||| ||  | | ||  |     | | | | | || || |  ||   |   ||  | ||   | || ||   | ||  ||   ||  | ||                                          
AAGAGGTGCACTTAGAGCAGAGAGACCAGAGCTTCTTACAGTTATCTTACCTCAAAGTTTGAAAAAGCAACCTCCTGAAAGCCAGGAACTACTGTCAAAAgtaagagactagtttcaagattttcttttactccaactgcattgtcttaaatatcgaa-atgttcacttctatctgctataaatctgaatatcatccattcttggctatgctatagttcaactgagtctcctttgtaatgtctgcaacag

upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: Vv05s0094g01380.t01 (Vitis vinifera), 5'ss of exon 5
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatattt-ctgttctgaagtgagactttttgatatttctattataaactggtcat-tttgtagtcaaacttttctcccgacctgttactt
 || || ||  ||||||| || || |||||| ||||||||||||| |||||||| ||| | |||| ||| |||||||| |||||||||||  ||||||||||| || |  || ||| || || || |    ||   | |||     || | | | | |  |  |||| |||  | |     | || ||  ||  |       
CAGAGGTGCGCTAAGAGCAGAGAAACCAGAGCTGCTTACTGTAATCTTACCTCAGAGTTTGAAGATGCAACCTCCTGAGAGCCAAGAACTACTGTCTAAAgtaggactttatcataattgctattgtttcatgcatcatttc----ttgcaaatgtga--taatcatatttagaatgtttttcttttcatgattttcag---

upper sequence: GRMZM2G081653_T02 (Zea mays), 5'ss of exon 4
lower sequence: Vv10s0042g00120.t01 (Vitis vinifera), 5'ss of exon 3
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatatttctgttctgaagtgagactttttgatatttctattataaactggtcattttgtagtcaaacttttctcccgacctgttactt--------
 || || ||||| ||||| || || |||||  ||||||||||||| || ||||| ||| | || ||||| ||||| |||||||| |||||| |||||||||||||| |   | ||   | | |  ||   |   | ||||       | ||||  ||| |    |  ||     | | | || |       || | |||         
AAGAGGTGCTTTGAGAGCGGAGAAACCAGAACTGCTTACTGTAATCTTGCCTCAGAGTTTGAAAAAGCAACCTCCCGAAAGCCAGGAACTGCTGTCTAAAgtaagatttgctcatgactttctcttg---tttcatttttca-----tttattgaaaattaaatacctttgctgctatcattcccatgattctttcactgtcaaaggt

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|89764107|gb|DY691035.1|DY691035
EST:     CTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAA                         GTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTGATTGAG
genomic: CTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAG
EST: gi|149090139|gb|EE179439.2|EE179439
EST:     CTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAA                         GTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAG
genomic: CTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAG
EST: gi|78079193|gb|DV507605.1|DV507605
EST:     CTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAA                         GTACAAAACTTACTCGAGAAAACACAGTATGATCATTTGCCACTAAATGAG
genomic: CTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 37 (upstream exon), 45 (downstream exon)
Score: 23

TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAGGCTAGCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 79

TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAGGCTAGCAG


Block sizes: 42 (upstream exon), 45 (downstream exon)
Score: 27

TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAGGCTAGCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 94

TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatcc ... ttttttatagGTACAAAACTTACTCGAGAAACCACAGTATGATCATTTGCCACTAATTGAGGCTAGCAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TAGGGGCGCTTTAAGAGCTGAAAAGCCAGAGTTGCTTACTGTAATTTTACCTCAAAGTCTAAAGAAGCAGCCTCCTGAAAGCCAAGAACTGTTGTCTAAAgtaagaatccatgatatttctgttctgaagtgagactttttgatatttctattataaactggtcattttgtagtcaaacttttctcccgacctgttactt

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAC