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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatgagctgcattttgtcatttcatttccacctgtttgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag

Basic information

species Zea mays
transcript GRMZM2G081462_T01
intron # 4
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G081462_T01 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os01g17170.1 (Oryza sativa), 5'ss of exon 4
GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtat--aaatgagctgcattttgtcattt-catttccacctgtttgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag
|||||||||||||||||||| ||||| |||||||| ||| |||||||||| || |||||||||||||||||||||||||||||||||||||| || ||||||   |||| | || | |||| |  ||| ||     |  | | |||| |||    || |   |  ||   ||  | |||  |  ||  | |    ||| | |                                                                                             
GTGTATGTGACCATGTACCTAAATGATTGCCAACGTACTACATTCTATGAAGGGATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATAATTGAGgtaagaaaatcaacttccttttcttgtttacaaaattaattatgtgccctac----tttactgtagtccagactgtttcagaa-gaattgctgaatattaaaaca-------------------------------------------------------------------------------------------

upper sequence: GRMZM2G081462_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA03G27380.1 (Glycine max), 5'ss of exon 4
GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatgagct-gcattttgtcatt----tcatttccacctgtttgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag
|| ||||| || ||||| || ||||| |||||||| || || ||||| || || ||||| ||  |||| |||||||| ||||||||||| || ||||||||     || | | |||||||   ||      |||| |  | |  ||    |  ||| |  ||| || | |  |     ||   |  | |  | |  ||||                                                                                                 
GTTTATGTCACAATGTATCTCAATGATTGCCAACGTACAGCTTTCTACGAAGGCATTGGACTTAACACAAAAGAATTTGACATGCATGTCATTATCGAGgttagtttgtgtttgcatttttctataatcaccattcctccattgttattttgtataatcaatatttgtttttatccattctcaattaca--taatcaattt------------------------------------------------------------------------------------------------

upper sequence: GRMZM2G081462_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA19G30350.1 (Glycine max), 5'ss of exon 4
GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatgag-ctgcatttt---gtcatttcatttccacctgtt--tgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag
|| ||||| || ||||| || ||||| |||||||| || || |||||||| || ||||| ||  |||| |||||||| ||||||||||| |||||||||||     || | |||||||||    | ||  |  ||||  | |||  ||   |  ||| |   || || | |   | ||    | | ||    ||  |||||                                                                                                 
GTTTATGTTACAATGTATCTCAATGATTGCCAACGTACAGCTTTCTATGAAGGCATTGGACTTAACACAAAAGAATTTGACATGCATGTCATCATCGAGgttagtttgtgtctgcatttttctataatcaccattccctccgttgatgttttgtataatcggtatttgttt---ctattcattcaaaattacttgcgaattt------------------------------------------------------------------------------------------------

upper sequence: GRMZM2G081462_T01 (Zea mays), 5'ss of exon 4
lower sequence: Vv08s0040g00390.t01 (Vitis vinifera), 5'ss of exon 4
GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatgagctgcattttgtcatttcatttccacctgtttgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag
|| ||||| || |||||||| ||||| ||||| || || |  |||||||| |||||||| ||  |||| |||||||| ||||||||||||||||| |||||         |||||| ||       |||  || || | ||     |  |  |   || |  || ||   |||   |||  |||  ||     ||  ||                                                                                             
GTTTATGTAACAATGTACCTCAATGATTGCCAGCGTACGGATTTCTATGAAGGAATTGGGCTCAACACAAAAGAATTTGACATGCATGTGATCATAGAGgtg-----agcctgcatatt-------catgccctccagctttttccatgttataagtaatgatccgagtcatgc--ttatgaaatttt---gttttgcag--------------------------------------------------------------------------------------------

upper sequence: GRMZM2G081462_T01 (Zea mays), 5'ss of exon 4
lower sequence: EFJ28505 (Selaginella moellendorffii), 5'ss of exon 4
GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtat----aaatgagctgcattttgtcatttcatttccacctgtttgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag
||||| ||||| |||||||| |||||||||||| | ||||| || || || ||||| || || ||||| ||||| |||||||||||||| |||||||||||||    || |||| || |   |   |  |||    |   ||||| |  |  | ||                                                                                                                                            
GTGTACGTGACAATGTACCTAAATGACTGCCAAAGAACTGCCTTTTACGAAGGAATAGGCCTCGACACTAAAGATTTCGACATGCATGTCATCATCGAGgtattttaaagtgagatg-agaataggaactcacgatcgtttgttttctctg-acag--------------------------------------------------------------------------------------------------------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32848943|gb|CD988624.1|CD988624
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|14245233|gb|BG873815.1|BG873815
EST:     TGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: TGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32925791|gb|CF030603.1|CF030603
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32926514|gb|CF031326.1|CF031326
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32849213|gb|CD988894.1|CD988894
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGGTCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32849632|gb|CD989313.1|CD989313
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32849875|gb|CD989556.1|CD989556
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|76016942|gb|DT944112.1|DT944112
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32850465|gb|CD990146.1|CD990146
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32828872|gb|CD968550.1|CD968550
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32849037|gb|CD988718.1|CD988718
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGTTCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|13562192|gb|BG550412.1|BG550412
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|16917031|gb|BM073096.1|BM073096
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32924699|gb|CF029511.1|CF029511
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32926437|gb|CF031249.1|CF031249
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|1472939|gb|AA011845.1|AA011845
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32849757|gb|CD989438.1|CD989438
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32859055|gb|CD998736.1|CD998736
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32848903|gb|CD988584.1|CD988584
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|33100033|gb|CF059993.1|CF059993
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32926083|gb|CF030895.1|CF030895
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|13198178|gb|BG354191.1|BG354191
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|213172608|gb|FM194596.1|FM194596
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACNAACCGCACAACGNCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|32926792|gb|CF031604.1|CF031604
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|13558924|gb|BG550279.1|BG550279
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|211289648|gb|FL131016.1|FL131016
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATTGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|78107168|gb|DV525586.1|DV525586
EST:     AGGGAATTGGTCTGGACACCCAAGAATTCGACATGCATGTGATCATCGAG                         ACCACCCGCACCACGGCGAGGATCCTCCCTGCTGTCCTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC
EST: gi|101401160|gb|EB822258.1|EB822258
EST:     AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAG                         ACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCTTAGACGTCGAGAAC
genomic: AGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 3 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 89

GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAACCCAGAGTTCAAGAGGAAGCTTGACAGGATGGTTGAGATCAACCAGAAGA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 940

GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAACCCAGAGTTCAAGAGGAAGCTTGACAGGATGGTTGAGATCAACCAGAAGA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 479

GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatga ... acccatgcagACCAACCGCACAACGGCGAGGATCTTCCCTGCTGTCCTAGACGTCGAGAACCCAGAGTTCAAGAGGAAGCTTGACAGGATGGTTGAGATCAACCAGAAGA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTGTATGTGACCATGTACCTGAATGACTGCCAACGCACTGCATTCTATGAGGGAATTGGTCTGGACACCAAAGAATTCGACATGCATGTGATCATCGAGgtataaatgagctgcattttgtcatttcatttccacctgtttgccatacatagttcatacttttctgaacaatgtcattacaaaaggttaggaattcacatgaaatttcttagagttcgccgtcgacgcttgggagaatctatgatttgcttctagaaatgttggcgtgaattaaatatgcgtacccatgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA