ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaattagtacacaactccattctacgtgcaccagaatgttgaacgtccatattatttcttcaaaatttattttagctttcctgtagaggagtcc...

Basic information

species	Zea mays
transcript	GRMZM2G052855_T01
intron #	4
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G052855_T01 (Zea mays), 5'ss of exon 4
lower sequence: LOC_Os05g36110.4 (Oryza sativa), 5'ss of exon 4

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaattagtacacaactccattctacgtgcaccagaatgttgaacgtccatattatttcttcaaaatttattttagctttcctgtagaggagtcc------
||||| ||||| ||| |||||||||||||||| ||||||||||| ||||||||||||||||| |||||||| |||||||||||||||||||||||||||||| || | ||  ||| | |   | | |||| ||||     |||  |  ||  ||||  ||||||||   || | |||   | ||| |  | |  | | |       
TCAACTGTCTTTTGGATTGGAGAATGGTTTATTAAGAAAATGCCTTTTGTAAGGCACATATATTCTGCATCGAAGCAAGTTAGCACTGCTGTTTCACCAGgtgat-tcatacgtatata---caaatctatgtgctttgaaatagtctac--ccatgatatttctttttaactcattcatgtttttcacttggagtgacacattga

upper sequence: GRMZM2G052855_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA06G15360.2 (Glycine max), 5'ss of exon 4

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgta---attagtacacaactccattctacgtgcaccagaatg----ttgaacgtccatattatttcttcaaaatttattttagctttcctgtagaggagtcc
 | || || |||||| ||||||| ||||| ||||||  ||||||| |||| ||||| |||||||||||||| |||||  ||||  ||||  | || ||||||| | |    || | | |  |   | |||     |   ||||||      ||||  ||   |   | |||      ||      ||     | | |||        
GCCACTGTTTTCTGGATTGGAGAGTGGTTCATAAAGCGAATGCCCCTTGTTAGGCATATATACTCTGCATCCAAGCAGATTAGTGCTGCAATATCTCCAGgtattctctacataactgcgtagtccaattaggatttagccagaaaagagtttgatggtgggtccgaattcacatggatgagcagtaagccacatatagg-------

upper sequence: GRMZM2G052855_T01 (Zea mays), 5'ss of exon 4
lower sequence: GLYMA04G39490.1 (Glycine max), 5'ss of exon 4

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaa--tgta-attagtacacaactccattctacgtgcaccagaatg----ttgaacgtccatatt-atttcttcaaaatttattttagctttcctgtagaggagtcc
 | || || |||||| ||||||||||||| ||||||  ||||||| |||| ||||| |||||||||||||| |||||  | ||  ||||  | || |||||||   | || || | | || |   | |||  |  |   ||| ||      ||||  ||   |  | | ||| |    ||      ||     | |||||        
GCCACTGTTTTCTGGATTGGAGAATGGTTCATAAAGCGAATGCCCCTTGTTAGGCATATATACTCTGCATCCAAGCAGATCAGTGCTGCAATATCTCCAGgtatgctctacataactgcatagtccaattagaatttagccataaaagagtttgatggtgggttctgaattcatatggatgagcagtaagccacatgtag--------

upper sequence: GRMZM2G052855_T01 (Zea mays), 5'ss of exon 4
lower sequence: AT1G43130.1 (Arabidopsis thaliana), 5'ss of exon 4

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaa---tgta-attagtacacaa-ctccatt--ctacgtgcaccagaatgttga-acgtccatattatttcttcaaaatttattttagctttcctgtagaggagtcc
|| || || |||||| |||| ||   |||||||| |  |||||||||||| | ||||||||| || || || |||||| ||||||||||| |||| ||||||||   |||  | |  | ||    || ||     | | |||   ||  | ||    | |||| | ||| | | |  ||||  | |   |||| ||  | |       
TCTACAGTTTTCTGGCTTGGGGAGCAGTTTATAAGGCGAATGCCCTTTGTCAAGCACATATATTCAGCTTCTAAGCAAATTAGCACTGCTATTTCTCCAGgtaagactgtgtactgtttcattgtcttcaccagttccctgctttagctttttctcgcatcca-aatatatttgctcaattcgtctc--cttttcttcaaata-----

upper sequence: GRMZM2G052855_T01 (Zea mays), 5'ss of exon 4
lower sequence: Vv07s0031g02010.t01 (Vitis vinifera), 5'ss of exon 4

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaattagtacacaactccattctacgtgcaccagaatgttgaacgtccatattatttcttcaaaatttattttagctttcctgtaga--ggagtcc---
 | || || |||||| ||||||||||||||||||||| |||||||||||| | ||| |||||||| || || |||||| ||||  | ||  |||| ||||||||      |          | |||| |  |   |||   |  || |||| | |  |  ||  | ||||| |  ||  |   |     || |  | | |     
GCCACTGTTTTCTGGCTTGGAGAATGGTTTATAAAGAGAATGCCCTTTGTTAAGCATATATACTCGGCCTCCAAGCAAATTAGTGCCGCAATTTCTCCAGgtaactc--ctgaagtttttttacatttt-tgcttacc--gactttcaacgcctagttgtttggtgcaaaaatacttgcattatgaacataaattgaaatttctg

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|93283406|gb|EB675670.1|EB675670

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|88752753|gb|DY536894.1|DY536894

EST:     CATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCTCCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: CATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|60397295|gb|DN230111.1|DN230111

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|148995086|gb|EE034390.2|EE034390

EST:     AAGGCACATATACTTTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|71319563|gb|DR796526.1|DR796526

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|148985826|gb|EE029793.2|EE029793

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|14245263|gb|BG873845.1|BG873845

EST:     CATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: CATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|91049872|gb|EB160290.1|EB160290

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|78109866|gb|DV528282.1|DV528282

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

EST: gi|89249400|gb|DY621186.1|DY621186

EST:     AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAG                         ATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG
genomic: AAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 66

TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaat ... ctctggacagATCAAAATACAACAGCATTCAAAGAAGTAGCAATCATTAGTCATCCCCGTGTTGGTGAATATGCATTTGGATTCATAACATCAACCATGGTTCTTCAG

Block sizes: 45 (upstream exon), 43 (downstream exon)
Score: 32

Block sizes: 49 (upstream exon), 43 (downstream exon)
Score: 29

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 98

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TCAACGGTCTTCTGGGTTGGAGAATGGTTTATAAAGAAAATGCCCTTTGTAAGGCACATATACTCTGCATCAAAGCAAGTTAGCACTGCTGTTTCACCAGgtaatgtaattagtacacaactccattctacgtgcaccagaatgttgaacgtccatattatttcttcaaaatttattttagctttcctgtagaggagtcc

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT