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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataacatgtgaaaactgctgctgatttgttgcagatttttttcccagtaacttatctagtttcaggtgttcattgtctatctgaagaagaaagaacaaatctaatttcag

Basic information

species Zea mays
transcript GRMZM2G025528_T04
intron # 4
splice site 5'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71323984|gb|DR798678.1|DR798678
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
EST: gi|148994757|gb|EE034229.2|EE034229
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGG
EST: gi|148957676|gb|EC903790.2|EC903790
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
EST: gi|149086713|gb|EE176255.2|EE176255
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAG
EST: gi|148953239|gb|EE152723.2|EE152723
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
EST: gi|71330461|gb|DR801989.1|DR801989
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCCT
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACC-T
EST: gi|148982279|gb|EE028228.2|EE028228
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
EST: gi|32923995|gb|CF028807.1|CF028807
EST:     GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAG                         ATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG
genomic: GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 45 (downstream exon)
Score: 32

GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTGACAAAGCTGAGGCAGGCACTGCAGAGTACCTCATTGAGCTTGAAGAACG


Block sizes: 44 (upstream exon), 47 (downstream exon)
Score: 170

GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTGACAAAGCTGAGGCAGGCACTGCAGAGTACCTCATTGAGCTTGAAGAACG


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 183

GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTGACAAAGCTGAGGCAGGCACTGCAGAGTACCTCATTGAGCTTGAAGAACG


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 58

GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataac ... ctaatttcagATGCTCCCAAAGACTTGGAGGAGAGTGGAGCCTCTGGAATCCGGCAACCTGACAAAGCTGAGGCAGGCACTGCAGAGTACCTCATTGAGCTTGAAGAACG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GACAAGTGGAAATGTGGAACCAAGCAAACAAGCTGTAGACAGAGgtagtataacatgtgaaaactgctgctgatttgttgcagatttttttcccagtaacttatctagtttcaggtgttcattgtctatctgaagaagaaagaacaaatctaatttcag

- - AGTGGA
- - - - - - - - - -  AAGCAA
- - - - - - - - - - - - - - CAAGCT
- - - - - - - - - - - - - -  AAGCTG