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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg...

Basic information

species Zea mays
transcript GRMZM5G833032_T03
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G833032_T03 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os02g45670.1 (Oryza sativa), 5'ss of exon 3
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtg--------------gagcatcaaattcaccagcaccctttgtgttgggg
 ||||||||||||| || |||||||| || || |||||||||||||||||||||||||| |||||||| |||||||| ||||| ||||| ||||| ||||||    | |||||| |||| |||      |   |   | || |||||||  ||  |    | ||                | |||  | ||||  |       |  | |||  |
TTTTTGAAGGTTCAAAAGAATGGAACAAGTGAGCATGTCCCACCTCCACGACCAAAGCGTAAAGCTGCTCATCCATACCCTCAAAAGGCCTCCAAAAATGgtagatcctcatatgctcataagttcagcacgcacccaacacatatatctttgatactaacagtacccccctctgcacaaaacatttacttcaatatatattctcatcttgcag

upper sequence: GRMZM5G833032_T03 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA10G37520.1 (Glycine max), 5'ss of exon 3
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaata-atgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg
 || | || |||||||| | ||| || || |||||| | || || ||  |||||||  |||||||||  ||||||||||||||||| ||||| || |||||| |||      ||| |||  |    |     || |  ||    |  ||   | ||        | |    | | |||   |||                 
TTTCTAAAAGTTCAGAAGAGTGGGACAAGTGAACATCTTCCTCCACCCAGACCAAAAAGAAAAGCTGTTCATCCATATCCTCAGAAAGCTTCAAAAAATGgttaga------atcttcactatattttttgctacacaatttagtgcatttgtatctaata--attgttagtgatatttggcag-----------------

upper sequence: GRMZM5G833032_T03 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA20G30250.1 (Glycine max), 5'ss of exon 3
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgaga--cttca---tatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg
 || | || |||||||| | ||| || |  |||||| | || || ||  |||||||  |||||||||| ||||||||||||||||| ||||| || | |||| | |  |||||   |||  |           | |  | | |    || | ||  |   |   | ||  |||      |||   |||                 
TTTCTAAAAGTTCAGAAGAGTGGGACAAATGAACATCTTCCTCCACCCAGACCAAAAAGAAAAGCTGCTCATCCATATCCTCAGAAAGCTTCAAAAACTGgttatagtcttcactatattttttttttttgctacacaatttagtgcatttgtctctaataattgttggtgag------atttggcag-----------------

upper sequence: GRMZM5G833032_T03 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA09G24400.1 (Glycine max), 5'ss of exon 3
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg
 || | || |||||||| | ||| || || |||||| | ||||| ||  | || ||  ||||||||||||||||||||||||| || |||||||| |||||| | | |   | ||  ||       |  |   |||  | |  |  ||  || ||      | |  |   | | ||    |||                 
TTTCTAAAAGTTCAGAAGAGTGGGACAAGTGAACATCTTCCACCACCCAGGCCTAAAAGAAAAGCTGCCCATCCATATCCTCAAAAAGCTTCCAAAAATGgtcataatc--tttcaccatttcttttttagtcaat--ttaagtgcatttatatctgatattttataat-tgatatctggcag-----------------

upper sequence: GRMZM5G833032_T03 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA16G29740.1 (Glycine max), 5'ss of exon 3
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg
 || | || |||||||| | ||| || || |||||| | ||||| || || || ||  ||||||||||||||||||||||||| || |||||||| |||||| | | |   | ||  ||       |  | | ||   | |  |  ||  || ||      | |  |   | | ||    |||                 
TTTCTAAAAGTTCAGAAGAGTGGGACAAGTGAACATCTTCCACCACCCCGGCCTAAAAGAAAAGCTGCCCATCCATATCCTCAAAAAGCTTCCAAAAATGgtcataatc--tttcaccatttattttttagacaac--ttaagtgcatttatatctaattatttataat-tgacatctggcag-----------------

upper sequence: GRMZM5G833032_T03 (Zea mays), 5'ss of exon 3
lower sequence: AT5G52660.2 (Arabidopsis thaliana), 5'ss of exon 3
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg-----
 || | ||||| || || | ||| ||| | |||||| |||| ||||| ||||| ||  | ||||| || |||||||||||||||||||||  ||||||||||   || | ||| |  | |      ||||||   |||  |  | |||   |||  |||  |   ||  |  | ||| |   ||||  ||| ||     |     
TTTCTTAAGGTACAAAAGAGTGGGACCGGTGAACATCTCCCTCCTCCTCGACCTAAAAGGAAAGCCGCTCATCCATATCCTCAGAAGGCTCACAAGAATGgt---acgttatagctactgc---tttaaaaactttga--agttgtattgttgtt-tgc-ttactgaaaactatattgat--gcactgtttatggcaatgtgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211267586|gb|FK993694.1|FK993694
EST:     ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATG                         ATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
genomic: ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
EST: gi|211267584|gb|FK993692.1|FK993692
EST:     ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATG                         ATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
genomic: ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
EST: gi|50330402|gb|CO525528.1|CO525528
EST:     ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATG                         ATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
genomic: ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
EST: gi|149081532|gb|EE170668.2|EE170668
EST:     ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATG                         ATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
genomic: ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
EST: gi|74234805|gb|DT642719.1|DT642719
EST:     ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATG                         ATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT
genomic: ACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 2 RNA-Seq libraries.


Block sizes: 42 (upstream exon), 26 (downstream exon)
Score: 6

CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCATGGGCTCATAGTTCCATCCCACAAGCTGTTGCTTCATCAATGGTGAAAG


Block sizes: 49 (upstream exon), 24 (downstream exon)
Score: 3

CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttc ... ctcaaaacagATTCATCTTCCATCCATAGGAACTCTGGCATGAACGTGCCTGTTTCTTCATGGGCTCATAGTTCCATCCCACAAGCTGTTGCTTCATCAATGGTGAAAG




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Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CTTTTGAAGGTTCAGAAAAATGGAACCAGCGAACATGTCCCACCTCCACGACCAAAGCGAAAAGCTGCCCATCCATATCCTCAGAAGGCTTCCAAGAATGgtgagacttcatatcctcagaaggcataaaaataatgatcatatatatccatgtcatgcgctgtggagcatcaaattcaccagcaccctttgtgttgggg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC