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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag

Basic information

species Zea mays
transcript GRMZM2G133464_T01
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os02g12580.1 (Oryza sativa), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
||| ||||||||||| ||||| ||||||||||||||||| ||||| || ||||||||||||||||||| ||||||||||||||||||||||||||| ||||| ||   ||  | |   ||  |||| | |||| |||||||||    ||||||| |||  ||||| |||  | || |||||||  ||
CTACGATGAATGCTTGAGAAAGTATGGAAATGCAAATGTATGGAAATACTTTACAGACTTGTTTGATTATTTGCCTCTCACAGCTCTTATAGAAAACCAGgtttgttacttgacta-attgcggttta-actgatataggcttatccaatatatgcct--gacatgtga--atcacccattttatag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA18G17100.1 (Glycine max), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
||||||||||||||| ||||||||||||||||| ||||||||||| || ||||||||||||||||||| ||||||||| || || || || || | |||||| || || | ||| |||  |  || | ||        | ||   |  ||     ||||| || | |   |    |   | ||||||
CTATGATGAATGCTTGAGAAAATATGGAAATGCGAATGTCTGGAAATACTTTACAGACTTGTTTGATTATTTGCCTCTGACTGCCCTCATTGAGAGTCAGgtttg-agttaaatgttgc-agagtggttatct-----gactaattttatgccggcctttaacttct---acctctgtgtattgcag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA08G40490.1 (Glycine max), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaa-acatccattttgcag
||||||||||||||| ||||||||||||||||| ||||||||||| || ||||||||||||||||||| ||| ||||| || || || || || | |||||| ||    | || | ||||   |   ||  |  | | | ||   |  ||     | ||| || | || | | |||            
CTATGATGAATGCTTGAGAAAATATGGAAATGCCAATGTCTGGAAATACTTTACAGACTTGTTTGATTATTTACCTCTGACTGCCCTCATTGAGAGTCAGgtttgag--ttaaatatgttgcag--aatgttcatct-gactaattttatgccagcatttaacttctgtgtatacag-----------

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA01G04570.1 (Glycine max), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
||| ||||||||||| ||||| ||||||||||| ||||||||||| |  ||||| || |||||||||| ||| ||||| || || ||||| || | |||||| ||       | |||  |||| |  | | ||||      || |||| ||  |          | | | |  | |  || || |||
CTACGATGAATGCTTGAGAAAGTATGGAAATGCCAATGTCTGGAAATTCTTTACCGATTTGTTTGATTATTTACCTCTGACCGCCCTTATTGAGAGTCAGgtttg-------agtttaatatgttgcagaatgtt------tttctgactaccttatgccagtgtctaacatccttgtatattacag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA02G02990.1 (Glycine max), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
|||||| |||||||| ||||| ||||||||||| ||||||||||| |  || || ||||||||||||| ||| ||||| || || ||||| || | |||||| || || | |||      ||| |  | | ||||      |   ||| |   |          | | | |  | |  || || |||
CTATGACGAATGCTTGAGAAAGTATGGAAATGCCAATGTCTGGAAATTCTTCACCGACTTGTTTGATTATTTACCTCTGACTGCCCTTATTGAGAGTCAGgtttg-agttaaat------atgttgcacaatgtt------tctttgactgccttatgccagtgtctaacatccttgtatgttacag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: AT1G59830.2 (Arabidopsis thaliana), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag-----
 ||||| |||||||| || ||||| |||||||||||||| |||||||||||||| ||| | || |||| | | ||||| ||||| || ||||| | ||||||  | ||||   | |||  |  || |  | |||   |  | |  |   |  |   |    || |||    | || |  | || |       
TTATGACGAATGCTTGAGGAAATACGGAAATGCAAATGTGTGGAAGTATTTTACGGACCTTTTCGATTATCTCCCTCTTACAGCACTCATAGAGAGTCAGgttcg-agct-gttcttgcca--gtcgtcattgtatcatccgtagttgtttcacttccgc-acttttcttcatcaactgtattccgtgacag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: AT1G69960.1 (Arabidopsis thaliana), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggc-------ttgctgaatatatccctttgacatttg--agaaacatccattttgcag---
 ||||||||||| || || |||||||||||||| ||||| |||||| | || || ||  | ||||||| | | || || ||||||||||| || | ||||||||  |    ||  |  | |  |||   |||    ||| |       |  |||  | |     ||||   ||    | | |  ||  |||| | |   
TTATGATGAATGTTTGAGGAAATATGGAAATGCTAATGTATGGAAGCACTTCACTGATCTTTTTGATTATCTTCCACTTACAGCTCTTATTGAGAGTCAGgtatttaa--aaaactaatgagcgttttcactaccttagacaatctcttctctgtttctgctgttttggacttgactacagaactctttttttctgtag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: AT1G10430.1 (Arabidopsis thaliana), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatact-gttataggcttg-ctgaatatatccctttgacatttgagaaacatccattttgcag
 ||||| |||||||| || || || |||||||| || ||||||||||||||||||||| | || |||| | | ||||| || || || ||||| | |||||| |  ||||   || | |    |||| |  | | || |   ||  || | || | |  |||||| ||    |||      ||||| ||
TTATGACGAATGCTTGAGGAAGTACGGAAATGCTAACGTCTGGAAGTATTTTACAGACCTTTTCGATTATCTTCCTCTTACCGCCCTCATAGAGAGTCAGgt-tagagct--gttctttctcagttggtgtttgatacatctttttctaagtacagctttttgac-ttcatcaaa------ttttgtag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: Vv01s0026g01080.t01 (Vitis vinifera), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgca--gctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
 ||||| |||||||| ||||||||||||||||| |||||||||||||||||||| ||| | ||||||| | | || || ||||| || || || | |||||| ||    |  ||  ||| ||||| ||  |||| | |||   || |||| |  || |   |||||       |   |  ||     ||
TTATGACGAATGCTTGAGAAAATATGGAAATGCCAATGTCTGGAAGTATTTTACCGACCTTTTTGATTATCTACCCCTTACAGCCCTCATTGAGAGTCAGgtttgagttgtccatgtttcttatgttt--tactttgataaatttcctgactg-atac---tgacagccatatattgtttataccatag

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: Vv14s0066g02240.t01 (Vitis vinifera), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
 ||||||||||||||||||||||||||||||||||||||||||||  ||||||| ||  | |||||||   | || |||||||| ||||| || | |||||| || | |||   ||  || ||  |  |  |||  | |  |   |  || |     | || ||     ||    |  || | |   
TTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAACATTTTACTGATCTCTTTGATTACCTACCCCTCACAGCGCTTATTGAGAGTCAGgtctg-atctc---ttgattttgcgtatttttgtgtttgattcaatccatgttaatttatgccaaccttgatttgtttatgtag---

upper sequence: GRMZM2G133464_T01 (Zea mays), 5'ss of exon 3
lower sequence: Vv17s0000g05330.t01 (Vitis vinifera), 5'ss of exon 3
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgca--gctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag
 |||||||||||||| ||||||||||| ||||||||||||||||| ||||| || ||| | ||||||| | | ||||| ||||||||||| || |  ||||| || |      |||  |    |  ||| |    |||    |||  |||  |  |   ||     ||||                   
TTATGATGAATGCTTGAGAAAATATGGGAATGCAAATGTCTGGAAATATTTCACCGACCTTTTTGATTATCTACCTCTTACAGCTCTTATTGAGAGCCAGgtttgaattatataatgcttccttatttggtttccttaatatcttattgacaacctttattgtgtctttgtag----------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67023817|gb|CO452566.1|CO452566
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCACGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|211266961|gb|FL418823.1|FL418823
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|211140423|gb|FL389577.1|FL389577
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAC                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|213179216|gb|FM192859.1|FM192859
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCACGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|211520000|gb|FL116080.1|FL116080
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|211266989|gb|FL420581.1|FL420581
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|60400032|gb|DN232839.1|DN232839
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|91870241|gb|EB400776.1|EB400776
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|148963286|gb|EE014276.2|EE014276
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|148963285|gb|EE014275.2|EE014275
EST:     CTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: CTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|76293399|gb|DV032967.1|DV032967
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|148944407|gb|EC889516.2|EC889516
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|71431047|gb|DR812097.1|DR812097
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|78083760|gb|DV512153.1|DV512153
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|78125124|gb|DV543508.1|DV543508
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCGG                         GTCTTCTGTCTTCGTGGTGGCCTCTCTCCGTCTTTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
EST: gi|148951611|gb|EC897351.2|EC897351
EST:     TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAG                         GTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT
genomic: TTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 103

CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCGCGTACAGGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 95

CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCGCGTACAGGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 98

CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCGCGTACAGGAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 68

CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagct ... cattttgcagGTCTTCTGTCTTCATGGTGGCCTCTCTCCGTCATTGGACACGTTGGATAATATTCGTTCTCTTGATCGCGTACAGGAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CTATGATGAATGCTTAAGAAAATATGGAAATGCAAATGTCTGGAAGTATTTTACAGACTTGTTTGATTTTTTGCCTCTCACAGCTCTTATAGAAAATCAGgtatgcagctcaattttgttatggttgatactgttataggcttgctgaatatatccctttgacatttgagaaacatccattttgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTC