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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctgtttcctgttaaattttaccattctcttttgtgaaaatagtggacacttgcaccagttaggtttttttttgtgctgtgagaagcacagaaa...

Basic information

species Zea mays
transcript GRMZM2G130548_T01
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G130548_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os02g51290.1 (Oryza sativa), 5'ss of exon 4
AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctgttt-----cctgttaaattttaccattctcttttg--tgaaaatagtggacactt--gcaccagttagg---tttttttttgtgctgtgaga--agcacagaaa------------------------------------------------
||  |||||||| |||  |||||| |||||||| || |||||||| ||||| |||||||| ||||||||||||| ||||||||| | ||| ||||||||||||||||||  ||      |  || ||  |||  |||||  | ||     || ||||   |  ||   | || | |||     ||| ||||  | ||| | |  ||     ||                                                 
AAGAAAGGATGAGTCAGATAGACCCGATGATGTGATATCAGCTGCGCAGAGATACATTGAACAAAATGGATCAAGAGCGTTTGAAAGTCTGGTTAACAAGgtattatctacttatggatccatttaaagttagaattcttctgtgaacagaagtagtcccctttttgtctcctgctagcgacctttctttttgggtgtcatatcagagtgcaacctacttatgtgttttaatctttggcacataacatttcaattcatgcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|5455802|gb|AI833492.1|AI833492
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|30052458|gb|AI065747.2|AI065747
EST:     ATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: ATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|29945353|gb|CB815596.1|CB815596
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|78027480|gb|DV495867.1|DV495867
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|111145857|gb|EE258097.1|EE258097
EST:     GGTACATTGAGCAAAATGGATCACAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|32831864|gb|CD971542.1|CD971542
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTATGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|22489673|gb|BU049596.1|BU049596
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|211320303|gb|FL454022.1|FL454022
EST:     GGTACATTGAGCAAAATGGWTCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|18649960|gb|BM498779.1|BM498779
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|32865321|gb|CF005003.1|CF005003
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
EST: gi|211023896|gb|FL457615.1|FL457615
EST:     GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAG                         TTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA
genomic: GGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 213

AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAAAGGAGGGCCAGGATTCAACTAGAATCTGAAGCGAGGGAAGTGAACCCTT


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 106

AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAAAGGAGGGCCAGGATTCAACTAGAATCTGAAGCGAGGGAAGTGAACCCTT


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 131

AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAAAGGAGGGCCAGGATTCAACTAGAATCTGAAGCGAGGGAAGTGAACCCTT


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 107

AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctg ... catcgtgcagTTTAAGAGTTCGAACCCAAGGCGCTCAATTCTGGAGGAGGTAGAGGTTGAAAGGAGGGCCAGGATTCAACTAGAATCTGAAGCGAGGGAAGTGAACCCTT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AAAGAAGGATGAATCAAGTAGACCTGATGATGTAATTTCAGCTGCACAGAGGTACATTGAGCAAAATGGATCAAAAGCGTTTGAGAATCTTGTTAACAAGgtattatctgtttcctgttaaattttaccattctcttttgtgaaaatagtggacacttgcaccagttaggtttttttttgtgctgtgagaagcacagaaa

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gaaaatag