ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CCTGGGACGCGGGACTCGTGGTCGGAGATGGACGACGCGAGGTCCAGTAGGACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtttcatactgatctcatcttaggaacccgcagtccttctgtgtgcttccccaatcgtgcttggatgtgttctgatgacagcagcaatcacaattgtttctgcag

Basic information

species	Zea mays
transcript	GRMZM2G119359_T01
intron #	3
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G119359_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os06g02560.3 (Oryza sativa), 5'ss of exon 3

CCTGGGACGCGGGACTCGTGGTCGGAGATGGACGACGCGAGGTCCAGTAGGACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagtt--gtttcatactgatctcatcttaggaacccgcagtccttctgtgtgcttccccaatcgtgcttggatgtgttctgatgacagcagcaatcacaattgtttctgcag
||||||| ||| |||||||||||||||||||| || | ||||||||    ||||||||||||||| ||||||||||| |||||||| |||||||||||||||| ||    | ||   | |     | |||  | | ||     ||  |  |   ||  | ||||  |  ||  || |  | ||||||  ||||                     
CCTGGGAGGCGAGACTCGTGGTCGGAGATGGATGAGGAGAGGTCCAACCAGACCTCCTTCTCGACAACCCAGCTCTCGATCTCCATCCCGATGCCCAGATgtgggtcccctatcggtccgcgtctaccttgagcatcccttcaccaacatttctctcacacaat--tcattccattttctttgatgat-gcag---------------------

upper sequence: GRMZM2G119359_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os06g02560.2 (Oryza sativa), 5'ss of exon 3

CCTGGGACGCGGGACTCGTGGTCGGAGATGGACGACGCGAGGTCCAGTAGGACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgttt--catactgatctcatcttaggaacccgcagtccttctgtgtgcttccccaatcgtgcttggatgtgttctgatgacagcagcaatcacaattgtttctgcag
               ||||||||||||||||| || | ||||||||    ||||||||||||||| ||||||||||| |||||||| |||||||||||||||| ||    |  |   | |     | |||  | | ||     ||  |  |   ||  | ||||  |  ||  || |  | ||||||  ||||                     
---------------TCGTGGTCGGAGATGGATGAGGAGAGGTCCAACCAGACCTCCTTCTCGACAACCCAGCTCTCGATCTCCATCCCGATGCCCAGATGTGGGTCCCCTATCGgtccgcgtctaccttgagcatcccttcaccaacatttctctcacacaat--tcattccattttctttgatgat-gcag---------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211397232|gb|FL471406.1|FL471406

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTAATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|211473705|gb|FL261296.1|FL261296

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCACATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|78024286|gb|DV492673.1|DV492673

EST:     GACCTCCTTCTNGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCACATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|78105391|gb|DV523809.1|DV523809

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|5018253|gb|AI714446.1|AI714446

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCACATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|211397231|gb|FL471405.1|FL471405

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTAATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|71767062|gb|DR964999.1|DR964999

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|149099681|gb|EE185730.2|EE185730

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

EST: gi|211473706|gb|FL261297.1|FL261297

EST:     GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGAT                         GTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCACATGA
genomic: GACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 108

CCTGGGACGCGGGACTCGTGGTCGGAGATGGACGACGCGAGGTCCAGTAGGACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtt ... gtttctgcagGTGATTGAGAACGAAGCTCGTGCGCGGCCAGTAGGTGGCGTTGTCGCATGACGCGCACCCAGCATCGCGCTCGCTGCTAGCGAGCCACCGTCACCCGGTG

Block sizes: 48 (upstream exon), 20 (downstream exon)
Score: 4

Block sizes: 47 (upstream exon), 43 (downstream exon)
Score: 15

Block sizes: 47 (upstream exon), 43 (downstream exon)
Score: 33

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CCTGGGACGCGGGACTCGTGGTCGGAGATGGACGACGCGAGGTCCAGTAGGACCTCCTTCTCGACGACCCAGCTCTCCATCTCCATTCCGATGCCCAGATgtgagttgtttcatactgatctcatcttaggaacccgcagtccttctgtgtgcttccccaatcgtgcttggatgtgttctgatgacagcagcaatcacaattgtttctgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTC