ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgtttttttatttcttgtactctgcatttctatgatgttttttttgtctattttattgcataccaggcaaaggttttaaatttccggtgattgtttt...

Basic information

species	Zea mays
transcript	GRMZM2G108285_T01
intron #	3
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G108285_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os07g36190.1 (Oryza sativa), 5'ss of exon 3

GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgtttttttatttcttgtactctgcatttctatgatgttttttttgtctattttattgcataccaggcaaaggttttaaatttccggtgattgtttt-------
||||| |||||||||| || || ||||| ||||| || |||||||| |||||||| ||||| ||||||||||||||||| || ||||| ||||||||       ||||  |  | |||||| ||| ||  | |||  | | | ||||  ||||||  || |||| |  || || |   |   | | |||  |       
GCCATCTGATGGAAAAAATCGAATCTGAAAATCTACTTCATAGGGCTTTCAGTGTATTCCTGTTCAACTCAAAATATGAACTCCTACTCCAGgtttggaga---atttacta-attctgcacttccattttttttaatct-tgtattacattgca--cctggcacatattctatacaactattcactgtgctcccatgt

upper sequence: GRMZM2G108285_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA18G47770.1 (Glycine max), 5'ss of exon 3

GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgtttttttatttcttgtactctgcatttctatgatgttttttttgtctattttattgcataccaggcaaa--ggttttaaa--tttccggtgattgtttt
  || |||||||||||||||||  |||||||| |||| ||||| || |||||||| ||||| |||||||| || |||||| |||| ||||||||  |||| ||  ||  |  |  | ||  ||  |   | |  | |  | |  || || ||  |    |  |  |  | ||| |||    ||  || |   |   
-TCACTTGATGGAAAAGATTGAAGCTGAGAATTTGCTGCATAGAGCTTTCAGTGTATTCCTGTTCAACTCTAAGTATGAGTTGCTTCTTCAGGtaagtttcttcgttgatatttttttggcttcatcataagcctgtagtttacatcttgttttaatgtaaacctattgatttcaaaagcatcaagtaaactt---

upper sequence: GRMZM2G108285_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA09G38550.1 (Glycine max), 5'ss of exon 3

GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgtttttttatttcttgtactctgcatttctatga-tgttttttttgtct-attttattgcataccaggcaaaggttttaaatttccggtgattgtttt
| || |||||||||||||||||  |||||||| |||| ||||| || |||||||| ||||| ||||||||||| |||||| |||| ||||||||  |||| || |||  |  |  | ||   |  || |  |||  |||   |||  |||||  | | |||      |  |   ||   ||  || |   |   
GTCACTTGATGGAAAAGATTGAAGCTGAGAATTTGCTGCATAGAGCTTTCAGTGTATTCCTGTTCAACTCAAAGTATGAGTTGCTTCTTCAGgtaagtttcttcattgatatttttttgacctcataagcctgtagtttacatcttgttttaacgtaaaccta-tttatttcaaaagcatcaagtaaacttgg-

upper sequence: GRMZM2G108285_T01 (Zea mays), 5'ss of exon 3
lower sequence: Vv04s0023g00600.t01 (Vitis vinifera), 5'ss of exon 3

GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgtttttttatttcttgtactctgcatttctatgatgttttttttgtctattttattgcata-ccaggcaaaggttttaaatt-tccggtgattgtttt
| |||||||||||||||||||| ||||||||| |||| || || || |||||||| || || || ||||| |||| |||  | || |||||||| |||  ||  ||||   | | |    | ||   || | |   |  |||   |   | |    | |  | | | | | || | |  | |  | |||||   
GTCATTTGATGGAAAAGATTGAATCTGAGAATTTGCTGCACAGAGCTTTCAGTGTATTTCTGTTTAACTCGAAATTTGAATTACTCCTTCAGgtatgtggttgaattttgggaagtgacaagtttcctgct-tgcatggtgtgggtgcaaatatggaacttgacgaggctgttcagtggttcatttattgtca-

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|33102488|gb|CF062448.1|CF062448

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|6527019|gb|AW216241.1|AW216241

EST:     GGGCATTCAGTGNGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAG
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAG

EST: gi|33101212|gb|CF061172.1|CF061172

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|33100629|gb|CF060589.1|CF060589

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|24765500|gb|CA400659.1|CA400659

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|60358782|gb|DN225755.1|DN225755

EST:     GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|84977083|gb|DW475505.1|DW475505

EST:     GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|24770101|gb|CA405230.1|CA405230

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|14203887|gb|BG837564.1|BG837564

EST:     GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|33101423|gb|CF061383.1|CF061383

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|18659800|gb|BM500528.1|BM500528

EST:     GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

EST: gi|33100900|gb|CF060860.1|CF060860

EST:     GGGCATTTAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAG                         CAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC
genomic: GGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 188

GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgttttt ... actcgtgcagCAAAGGTCCGCGACAAAGGTTACCTTTCCTTTAGTTTGGACCAATACCTGCTGCAGCCACCCTCTGTACCGTGAGTCTGAGCTTATCCAGGAGAACTACC

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 545

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 543

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 277

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GCCATTTGATGGAAAAGATTGATTCTGAGAATCTGCTACATAGGGCATTCAGTGTGTTCCTTTTCAACTCAAAATATGAGCTGCTACTTCAGgtttgtttttttatttcttgtactctgcatttctatgatgttttttttgtctattttattgcataccaggcaaaggttttaaatttccggtgattgtttt

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGCT