Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc...

Basic information

species Zea mays
transcript GRMZM2G104373_T01
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os03g18690.1 (Oryza sativa), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcag----------acatgaaag----ttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc--------------------
||||||| ||||||||||| ||||||||||| ||||||||||||||||||||||| ||||||||||||||||| ||||||||||| || || ||||||||||| |  ||          | |||  |     |  | |  |   || || ||||  |||   ||||   ||||| ||   ||  |    ||||   ||   | |  |||  | |                    
GTGATGGCCCCAAGCTTGTAAGAGAATTGTTTAGGGTGGCTGATGATCTTTCTCCGTCAATTGTCTTTATTGACGAGATTGATGCAGTCGGAACAAAGAGgtagagtagtgcatacttcaaatgggaagtcttctatatgatgaatgttgtttgtgtatagtaaaagattggtggaacttcccatgaaatagtttatatgag-tattgatgaacttttttttttctccttatag

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA10G04920.1 (Glycine max), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgta-tatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctgg--tgaaagggcctctagcctagtgg-----ttaaaggattctgagtagc------------------------
| ||||| || || ||||| |||||| | ||||| || || ||||||||||||||||| ||||| || |||||||| || ||||| ||||| ||||| ||||| | | |  |   ||  ||| | |   | |   |    ||  ||| || |  | ||   || |     || |   ||  |||     ||     |   | |  |||                        
GAGATGGCCCAAAACTTGTGAGAGAACTTTTCAGAGTTGCCGATGATCTTTCTCCTTCTATTGTATTCATTGATGAAATCGATGCAGTTGGTACAAAAAGgtaattttaacccccaactttagctgtcatgaaggtacc-ggggatggaagagtgatgccttgtatcactctatgagctcttgggattcttttcctacattcatgagcttaccagtaaatatgtatttacag

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA03G32800.1 (Glycine max), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgta---tatcagacatgaaagttaacagc----------agttattttct------ttggttatgaaaaa-------aagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc----------------------------------
| |||||||| |||||||| || ||| | ||| | || |||||||||||||| || || |||||||| ||||||||||| || |||||||| || ||||||||   |||   |||| |   |||  ||           ||  |||||||      ||  || |||             |  || | | |||   |  | || ||  |  |  |||     | | |                                  
GAGATGGTCCAAAGCTTGTAAGGGAACTTTTCCGAGTTGCTGATGATCTTTCCCCATCTATTGTCTTCATTGATGAGATAGACGCTGTTGGTACTAAGAGgtaagttattgaacatcagctttaggagtcactgtgtcaagacattttctatcttcttttttctgatttttttttccgggagggggcaggggatgcgggactggttatagagtgatatcatggatcaattcttagtatatgtctcttttatgcattgcag

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA19G35510.1 (Glycine max), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgta---tatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagg--gcctctagcct----agtggttaaaggattctgagtagc--------------------------------------------------------
| |||||||| || ||||| || ||| | ||| | || |||||||||||||| || || |||||||| |||||||| |||||||| ||||| |||||||||||   ||||    || |   |    ||    | ||||      || |  |       |      | |  |  | | |  |    ||  |  |  || | | |    |                                                         
GAGATGGTCCAAAACTTGTAAGGGAACTTTTCCGAGTTGCTGATGATCTTTCCCCATCTATTGTCTTCATTGATGAAATTGATGCCGTTGGTACAAAGAGgtaagttatcgagtatcagcttctggagtcactgttttaagacattttctatcctctttttccggatgtagttttttgttttgggaggaggcagggggtacaggactggtagaagagtgatatcatgagtcaattcttattatatgtctcttatatgctttgcag

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA13G19280.1 (Glycine max), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc-----------------------------------
| ||||| || || ||||| |||||| | ||||| || |||||||||||||||||||| ||||| || |||||||| |||||||| ||||| ||||| |||||  |   || |  || ||   || | | ||     | || |  |     |||   |       |  ||   |   | | |   ||||||||                                          
GAGATGGCCCAAAACTTGTAAGAGAACTTTTCAGAGTTGCTGATGATCTTTCTCCTTCTATTGTATTCATTGATGAAATTGATGCAGTTGGTACAAAAAGgtaattttaacctccaactt--tagtattcatgaaggtagggtggggttggaagagtgatgtcttgtatcactctatgagctcttaggattcttttcctacattcatgagcttactaataaatatgtatttacag

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: AT2G20140.1 (Arabidopsis thaliana), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga------catgaaagttaac----agcagttattttctttggttatgaaaaaaagctggtg----aaagggcctctagcctagtggttaaaggattctgagtagc
| ||||||||||||||||| || ||| | |||||||| |||||||| ||||||||||| ||||| || || || ||||||||||||||||| || ||| |||||  |  |      ||| ||  | | |       |   | | |  ||| || ||   || || ||||     | | |   | ||   | |||||| ||  |           
GAGATGGTCCCAAGCTTGTGAGGGAACTTTTCAGGGTTGCTGATGACCTTTCTCCTTCTATTGTTTTCATAGACGAGATTGATGCTGTTGGCACCAAGCGgtatgcctaaatacaacataaacatgatccattgataaacaatgttcttgattttgctcaagagatggtatctaatatgtgatataaaatggtggttgaaacag----------

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: AT4G29040.1 (Arabidopsis thaliana), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagac-atgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc
| |||||||| |||||||| |||||| | ||||| || |||||||| ||||| || ||||| ||||| || |||||||||||||||||||| || ||| ||||  | | || ||     | |||   |  |||   |  | |  || |     | ||| | |   |   | |     |   |                   
GAGATGGTCCTAAGCTTGTGAGAGAACTTTTCAGAGTTGCTGATGACCTTTCACCATCAATCGTCTTCATAGATGAGATTGATGCTGTTGGTACCAAGCGgtaagttaaactattttcataaacctgatctataacccattg--ataatggttatctgattacttgttgtttgaaagaacag-------------------

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: Vv07s0005g05160.t01 (Vitis vinifera), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc
| ||||| || ||  | || || || || || || || ||||||||||| || |||||||||||||| |||||||| |||||||| ||||| ||||||||||   ||    |||||  |||         | |    ||  | |        || |  |         | |    || |  | |||  ||||   | || 
GAGATGGGCCAAAATTAGTGAGGGAGTTATTTAGAGTTGCTGATGATCTCTCACCTTCAATTGTCTTCATTGATGAAATTGATGCAGTTGGTACAAAGAGgtgagtcg---atgaacattagtgatgaaaaatcatatttttca--------agttctt-------ttttttttttaattatcaaat-attccttgcag-

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: PP1S12_207V6.1 (Physcomitrella patens), 5'ss of exon 2
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattc--tgagtagc--
| ||||| || ||| | ||  |||| || ||  |||| || |||||  | |||||||| |||||||||||||| || |||||||| || || |||||||||||  |      |   | |||   | | | |   || ||   ||   ||     |   |   ||  |||||   ||   ||||   |  | |  || | | |  
GGGATGGACCTAAGTTGGTGCGAGAGTTATTTCGGGTCGCGGATGAATTATCTCCTTCCATTGTCTTTATTGACGAAATTGATGCAGTGGGTACAAAGAGgtacttttcgggtcctaattatt-gtaataagcatcatt--ctaattaagttctccattttgagcacctcttcgctga-ggttggcgattccattgcggaactt

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: PP1S4_277V6.1 (Physcomitrella patens), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggtt---aaaggattctgagtagc
| ||||| || ||| | ||  ||||  | ||  ||||||||||||| || |||||| | ||||| ||||| ||||| ||||||||||| || ||||| |||||  || | | ||    | |  |    |||||| |  || || | | ||      | |    |   |   |  ||   ||||   |  | | ||| |     
GAGATGGACCGAAGTTGGTGCGAGAGCTATTTCGGGTGGCTGATGAACTGTCTCCTACCATTGTTTTTATCGATGAAATTGATGCTGTCGGTACAAAAAGgtaattctggcctgcttctcatta-tttttatttgcggtgtttctcataatgtttcgattcttgcaaccacactctcaaggttgccactgcactcttatct--

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: EFJ20767 (Selaginella moellendorffii), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcag--acatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc
| || ||||| ||||||||||| ||  | ||  | || || || ||||||||||| || ||||| || ||||| ||||||||||||||||| || || ||||   |  |   ||||   |||  ||| |  || ||           |  || || ||  | ||                                      
GAGACGGTCCTAAGCTTGTTAGGGAGCTCTTTCGCGTTGCGGACGATCTTTCTCCGTCCATTGTGTTCATTGACGAGATTGATGCTGTTGGAACGAAAAGgtgatttcgttgcatggttgttg-cagaaactaatt-----------gggaacaaccttctcaag-------------------------------------

upper sequence: GRMZM2G104373_T01 (Zea mays), 5'ss of exon 3
lower sequence: EFJ32659 (Selaginella moellendorffii), 5'ss of exon 3
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcag--acatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc
| || ||||| ||||||||||| ||  | ||  | || || || ||||||||||| || ||||| || ||||| ||||||||||||||||| || || ||||   |  |  |||||   |||  ||| |  || ||           |  || || ||  |  |                                      
GAGACGGTCCTAAGCTTGTTAGGGAGCTCTTTCGCGTTGCGGACGATCTTTCTCCGTCCATTGTGTTCATTGACGAGATTGATGCTGTTGGAACGAAAAGgtgatttcgttacatggttgttg-cagaaactaatt-----------gggaacaaccttctctag-------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60394503|gb|DN227373.1|DN227373
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|87156235|gb|DY401024.1|DY401024
EST:     NCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTACGATGCTCATTCTGGTGGCGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|71318153|gb|DR795840.1|DR795840
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|61117980|gb|DN558941.1|DN558941
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|4887302|gb|AI677401.1|AI677401
EST:     TCT-CTTCAA-TGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTACGATGCTCATTCTGGTGGCGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|47961685|gb|CN844394.1|CN844394
EST:     GGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: GGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|60357188|gb|DN224161.1|DN224161
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|61117979|gb|DN558940.1|DN558940
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|76917636|gb|DV166930.1|DV166930
EST:     TTCAATTGTCTTTA-TGAATGAGGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TTCAATTGTCTTTATTG-ATGA-GATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|32847761|gb|CD987442.1|CD987442
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGTGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|78544201|gb|DV621699.1|DV621699
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTACGATGCTCATTCTGGTGGCGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
EST: gi|61117978|gb|DN558939.1|DN558939
EST:     TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAG                         GTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA
genomic: TCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 146

GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGAGTTGTTAAATCAGCTGGATGGTTTTGATTCAAGAGGAGATGTTAAAGTT


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 193

GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGAGTTGTTAAATCAGCTGGATGGTTTTGATTCAAGAGGAGATGTTAAAGTT


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 248

GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGAGTTGTTAAATCAGCTGGATGGTTTTGATTCAAGAGGAGATGTTAAAGTT


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 208

GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcaga ... cctgttgtagGTATGATGCTCATTCTGGTGGTGAGCGTGAGATTCAGAGAACCATGTTGGAGTTGTTAAATCAGCTGGATGGTTTTGATTCAAGAGGAGATGTTAAAGTT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTGATGGTCCCAAGCTTGTTAGAGAATTGTTCAGGGTGGCTGATGATCTTTCTCCTTCAATTGTCTTTATTGATGAGATTGATGCTGTTGGGACAAAGAGgtatatcagacatgaaagttaacagcagttattttctttggttatgaaaaaaagctggtgaaagggcctctagcctagtggttaaaggattctgagtagc

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gaaaaaaa