ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

AACTCTGTCTGGTACCCCTACCTGTGTCAGCTACCCAGCTACTACACTATCTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgtcaacaattttctccattatccatcttttgctgtgcactccaaaagcacccaacgatttttgttgttttcag

Basic information

species	Zea mays
transcript	GRMZM2G092131_T02
intron #	3
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G092131_T02 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os07g28840.1 (Oryza sativa), 5'ss of exon 3

----------AACTCTGTCTGGTACCCCTACCTGTGTCAGCTACCCAGCTACTACACTATCTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgtcaacaattttctccattatccatctt--ttgctgtgcactccaaaagcacccaacgatttttgttgttttcag
          |  ||    ||||| |  ||  ||| |||||| |||  |||||||||||||||||||||||||||||||||||||   ||||||||||||||| | |||||||| |||||| ||||  || |   || ||||| |   |||||      |       ||  || |    ||  || |||||||
AAAAGGGAAGAGTTCAAATTGGTATCTATATTTGTCTCAGCTGCCCTCCTACTACACTATCTTGGCTACCTTCAATGATTTTGAAACTGAAGCTCTCCAAgtacaactcggtagttctgtctacaaaatttta-gttttccatttcgattgcttcatgtt------acattcagc----ttacttcttttcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|91048511|gb|EB158929.1|EB158929

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|89252045|gb|DY623831.1|DY623831

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|71441696|gb|DR831412.1|DR831412

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|101390617|gb|EB817243.1|EB817243

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|71771111|gb|DR969048.1|DR969048

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|71435233|gb|DR816283.1|DR816283

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|101398534|gb|EB821140.1|EB821140

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|93015506|gb|EB641026.1|EB641026

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCG                         GTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|76932735|gb|DV173138.1|DV173138

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

EST: gi|149077854|gb|EE166581.2|EE166581

EST:     CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGT-T-----G                         ----ATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA
genomic: CTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 2 RNA-Seq libraries.

Block sizes: 40 (upstream exon), 30 (downstream exon)
Score: 3

AACTCTGTCTGGTACCCCTACCTGTGTCAGCTACCCAGCTACTACACTATCTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgt ... ttgttttcagGTTGATGATGCTATTTGGGTTGCACAAAAGGCTAAAAGTGCCATAAAATCAGACTGGGAAGATGCGACACCACTAATGAAAGAATTGGAATTTAAACCTA

Block sizes: 17 (upstream exon), 33 (downstream exon)
Score: 1

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AACTCTGTCTGGTACCCCTACCTGTGTCAGCTACCCAGCTACTACACTATCTTGGCTACCTTCAATGATTTTGAAGTCGAAGCTCTCCAAGTATATCTCGgtagatctgtcaacaattttctccattatccatcttttgctgtgcactccaaaagcacccaacgatttttgttgttttcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG