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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcctgcaatttcagtccagccgtcctgtattgtgatgttctggttggtccagttgagagtttggatttacagttcattgttgtccttcttttcgtgcag

Basic information

species Zea mays
transcript GRMZM2G086934_T01
intron # 3
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G086934_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os03g11540.1 (Oryza sativa), 5'ss of exon 3
GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtg---cctgcaatttcagtccagccgtcctgtattgtgatgttctggt-tggtccagttgagagtttggatttacagttcattgttgtccttcttttcgtgcag
 |||        |  | |   |      ||| ||  | ||| ||||||| ||||| ||||||||||||||||||||||||||||  |||||||| || ||||||| |   | ||| |  ||   ||| |  || |||   ||  | ||||| |   |    ||||         |  | | | |  ||| || | | || ||  |||||
TCTCCAAGCCCACGAACGCGCCGCCACTTCCTCCGGTTGTGCTGAAGCCCAAGCAGGAGGTGAAGTCCGCGTCCCAGATCGTGAACGAGCAGCGCGGCAAgtaagcgattcttgcgagatc--cccatctctcttgttcagttgt-ttctgatgcagaattgttggtgagactaacctgccgattcttgatgccttcctgtt--tgcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148943263|gb|EC888324.2|EC888324
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|211530807|gb|FL076080.1|FL076080
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|50331879|gb|CO527005.1|CO527005
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|78024319|gb|DV492706.1|DV492706
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|148976840|gb|EE025967.2|EE025967
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|32804204|gb|CD956440.1|CD956440
EST:     AAGCAAGAGGTGAAGTCTGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|78081808|gb|DV510220.1|DV510220
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|78024998|gb|DV493385.1|DV493385
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACA-GGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|5566971|gb|AI881882.1|AI881882
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
EST: gi|149086433|gb|EE175976.2|EE175976
EST:     AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAA                         TGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT
genomic: AAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 375

GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGATCACTCTGAACCCCTACCAGGGTAACTGGGTCATTAAGGTGCGGGTCACG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 192

GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGATCACTCTGAACCCCTACCAGGGTAACTGGGTCATTAAGGTGCGGGTCACG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 117

GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGATCACTCTGAACCCCTACCAGGGTAACTGGGTCATTAAGGTGCGGGTCACG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 73

GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcct ... tttcgtgcagTGCTGCTCCTGCCACGCGCCTTTCCATGACAAGGAGGGTCCATCCCTTGATCACTCTGAACCCCTACCAGGGTAACTGGGTCATTAAGGTGCGGGTCACG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GCTCAGTCGTGGCTGAGGAAACAAATTCTCCCCCACTCGTGATGAAGCCTAAGCAAGAGGTGAAGTCCGCGTCCCAGATCGTGACTGAGCAGCGTGGAAAgtaagtgcctgcaatttcagtccagccgtcctgtattgtgatgttctggttggtccagttgagagtttggatttacagttcattgttgtccttcttttcgtgcag

- - - - - - - - - - - - - - - - - - - - - - - - - AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCAG