ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc...

Basic information

species	Zea mays
transcript	GRMZM2G067985_T06
intron #	3
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os05g01600.2 (Oryza sativa), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcat-catctctttgatcccaatatgctgttgtcaactgtttatc---
|||||||||||||| ||||||||||||||||||||||| ||||| ||||| || || ||||| || ||||| || |||||||| ||||| |||||||||||||||     |  | ||  |   |||  | |  ||   |    |  |||    | |  || ||  | | ||||||   | |||||   |  ||| ||| |    
GAGAAGATGACCCAGATTATGTTTGAGACTTTCAATGTCCCTGCAATGTACGTCGCTATCCAGGCCGTGCTTTCCCTCTATGCCAGTGGCCGTACTACTGgtatgtatgaactcatctctgtcttttacttcaatgagg----agggtttgctcaattatgcacttgtatgatccacctctgctgccatacacttttttttaat

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA06G15520.2 (Glycine max), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|| || ||||||||||| ||||||||||| |||||||| |||||||||||||| || ||||| || || ||||| || ||||| ||||| || || |||||| ||  || ||  |||| |  || |     | ||||| |    |   |  |   |   |  | || ||||  | ||| |  |    |||          
GAAAAAATGACCCAAATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTGGCCATCCAGGCTGTCCTCTCCCTATATGCAAGTGGTCGCACAACTGgtttgtacttat--ttccatctgtct-----taattcactcctttgtcttca--cattgttctatccttgaatcaaatctttttgaatcag---------

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA08G19420.1 (Glycine max), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatcctt--cctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| || || ||  | || || ||||| |||||  | ||||| ||| ||      |  ||   |||   |||  |  |  | |  |    | ||  | |  |     ||    |||  ||   |||     |     |||| | | 
GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTGCCTGCCATGTATGTTGCAATTCAGGCTGTCTTGTCTCTGTATGCCAGTGGAAGAACTACAGgtttgtttctgtgattgatcttggatttaccaattttactatactgtggtcat-gtgatttcaattggattgggccagttatttgaataaatgctgtatgt-

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA05G32220.1 (Glycine max), 5'ss of exon 2

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatg-acctgatccttcctttagtttggcgttgattcagtttcatagttctagcta-gcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|| |||||||||||||| ||||||||||| |||||||| |||||||||||||| || ||||| || || |||||  | ||||| ||||| || || |||||| || |  | | | |   | | | ||     |  |  | |  |||  ||||  |   | ||||  || ||  ||  || |                     
GAAAAGATGACCCAAATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTGGCCATCCAGGCTGTTCTCTCCTTGTATGCCAGTGGTCGCACAACTGgtttgtatattaccatctgtcttgattcattcttttataatcacatcattctttccctgaatcaattcctt--tcaaaacag--------------------

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA15G05570.1 (Glycine max), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatcctt--cctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| || || ||  | || || ||||| ||||| |||||||| ||| ||      |  ||   |||   |||    || | |         |  | |   |   ||    |  | | ||    |||  | |   ||| |   | | 
GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTGCCTGCCATGTATGTTGCAATTCAGGCTGTCTTGTCTCTGTATGCCAGTGGACGTACTACAGgtttgtttctgtgattgatcttggatttactatttactataccgtggacatgtgatttcaattggat--taggtcagcttattatctgagcaaattcctgta

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA08G15480.1 (Glycine max), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatg-acctgatccttcctttagtttggcgttgattcagtttcatagttctagcta-gcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|| |||||||||||||| ||||||||||| |||||||| |||||||||||||| || ||||| || || |||||  | ||||| ||||| || || |||||| || |  | | | |   | | | ||     |  |  | |  ||| |||||  |   | ||||  || ||  |   || |                     
GAAAAGATGACCCAAATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTGGCCATCCAGGCTGTTCTCTCCTTGTATGCAAGTGGTCGCACAACTGgtttgtatatcaccatctgtcttgattcattcttttataatcacattgttctttccctgaatcaattcctt--ttaaaacag--------------------

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA04G39380.2 (Glycine max), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|| || ||||||||||| ||||||||||| |||||||| |||||||||||||| || |||||||| || ||||| || ||||| ||||| || || |||||| ||   |  |   ||| |  ||||    || ||  |||||  |  |   |       |  | ||  |||  | ||||   | |    ||| |      
GAAAAAATGACCCAAATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTGGCCATCCAAGCTGTCCTCTCCCTATATGCAAGTGGTCGCACAACTGgtttgtatttgta--tccatctgtttca-attcat--agttttgtcatcaca----ttgttctttccctgaatcaaatactttctga--aacag------

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: AT5G09810.1 (Arabidopsis thaliana), 5'ss of exon 3

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc
||||||||||| || || ||||||||||| |||||||| ||||||||||||||||| || || || || || || |||||||| ||||| ||||| || |||      |  | |||   |        |   |  | ||||  ||  | ||   ||     || |  || |   |  | |  | |||| ||| |      
GAGAAGATGACTCAGATCATGTTTGAGACATTCAATGTCCCTGCCATGTATGTTGCCATTCAGGCCGTTCTTTCTCTCTATGCCAGTGGTCGTACAACCGgt------tagttcttaactctaaacatccaagtctgagttatattat-cttcttacttgtatttacttaaagtcgttctctttttgt-aacag------

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: Vv04s0044g00580.t01 (Vitis vinifera), 5'ss of exon 4

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc
|| |||||||| ||||| |||||||||||||| ||||| ||||||||||||||||| || || || || || || || ||||| ||||||||||| ||||||  |   | |   |   ||| | ||||  ||  |||| |||  |  ||   | |   |   |  | |   | | ||  ||||  | |  |||       
GAAAAGATGACTCAAATCATGTTTGAGACTTTTAATGTGCCTGCCATGTATGTTGCCATTCAGGCTGTTCTTTCTCTATATGCCAGTGGGCGTACAACTGgttcgtattaaaa-tctttttggattggaatt--ttcatttttct--tttctgttgttagtgtagcctgagttctaacttgctcct-ttgactag-----

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: Vv12s0178g00200.t01 (Vitis vinifera), 5'ss of exon 2

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatg-acctgatccttcctttagtttggcgttgattcagtttcatagttctagctagcatcatctctttgatcccaatatgct---gttgtcaactgtttatc
||||| |||||||| |||||||||||||| ||||| || ||||||||||||||||| |||||||| ||||| || || ||||| ||||| ||||| || ||| ||   |  | |  | |      | |  |   | |      | |  || |   |       | || ||| ||  | ||| ||      || | |  ||    
GAGAAAATGACCCAGATTATGTTTGAGACCTTCAACGTACCTGCCATGTATGTTGCTATCCAAGCTGTGCTGTCTCTGTATGCCAGTGGTCGTACAACAGgtttgtgtcaaagcactgcaagttgtagaagcaaaatacaagacctttttgtgtgtgtgtgtgtgtcattgttcatattattcttgaaccgttagccatt----

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: EFJ17401 (Selaginella moellendorffii), 5'ss of exon 2

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctgatccttcctttagtttggcgttgattcagt--ttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc------------------------------------------------
|||||||||||||| || ||||| ||||| |||||||| || |||||||| || || |||||||| || || |||||||| || ||||| || ||||| ||||||   |  |  |  |  | | |||   | ||     |  || ||   | |  ||    |    |  |||      | ||| || |   |  || || |                                                 
GAGAAGATGACCCAGATCATGTTCGAGACCTTCAATGTCCCGGCCATGTACGTGGCGATCCAAGCAGTCCTTTCACTCTACGCGAGTGGACGCACTACCGgtatggagtttttttatcccttgcttgttctagacaagatggttgatgcgtgtttcttttcttcgttgcttgcgatagacatgatgcttcaagttggttctacttttgtttttccctctaacacgttgtatgatcgtggtttgtgatcag

upper sequence: GRMZM2G067985_T13 (Zea mays), 5'ss of exon 3
lower sequence: EFJ35077 (Selaginella moellendorffii), 5'ss of exon 2

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatg-acctgatccttcctttagtttggcgttgattcagt--ttcatagttctagctagcatcatctctttgatcccaatatgctgttgtcaactgtttatc-------------------------------------------------
|||||||||||||| || ||||| ||||| |||||||| || |||||||| || ||||||||||| || || |||||||| || ||||| || ||||| |||||| |  |  |  || | || | |||   | ||     |  || ||   | |  ||    |    |  |||      | ||| || |   |  || || |                                                  
GAGAAGATGACCCAGATCATGTTCGAGACCTTCAATGTCCCGGCCATGTACGTGGCAATCCAAGCAGTCCTTTCACTCTACGCGAGTGGACGCACTACCGgtatggagttttttttttcctttgcttgttctagacaagatggttgatgcgtgtttcttttcttcgttgcttgcgatagacatgatgcttcaagttggttctacttttttttcttctctctaacacgttgtatgatcgtggtttgtgatcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|213172202|gb|FM186044.1|FM186044

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|67028438|gb|CO457187.1|CO457187

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTTGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|22542171|gb|BU092609.1|BU092609

EST:     GCAATCCAAGCGG-GCTCTCACTTTATGCTAG-GGGCGTACTACTG                         GTATTGTACTCGACTCTGG-GATGG-GTGAGCCACACTGTGCCCATTTACG
genomic: GCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|31350149|gb|CD434506.1|CD434506

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|211469316|gb|FL184444.1|FL184444

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|5928698|gb|AW055915.1|AW055915

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGNGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|32862856|gb|CF002538.1|CF002538

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|61117779|gb|DN558740.1|DN558740

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|18659298|gb|BM500264.1|BM500264

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATT-ACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|211537826|gb|FL109200.1|FL109200

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|61117780|gb|DN558741.1|DN558741

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|22543167|gb|BU093605.1|BU093605

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTC
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTC

EST: gi|60345925|gb|DN212898.1|DN212898

EST:     AGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: AGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|211469308|gb|FL184436.1|FL184436

EST:     AGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: AGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|13490635|gb|BG517399.1|BG517399

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGTCTCACACTGTGCCCATTTA
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAG-C-CACACTGTGCCCATTTA

EST: gi|31250096|gb|CD219120.1|CD219120

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

EST: gi|211466787|gb|FL184427.1|FL184427

EST:     TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTG                         GTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG
genomic: TGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 173

GAGAAGATGACCCAAATTATGTTTGAGACTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCGGTGCTCTCACTCTATGCTAGTGGGCGTACTACTGgtatgacctg ... caatctacagGTATTGTACTCGACTCTGGTGATGGTGTGAGCCACACTGTGCCCATTTACGAAGGATATGCCCTTCCTCATGCCATTCTTCGTTTGGATCTTGCTGGTCG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 283

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 237