ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GTTGGGAGATACAAGAGCCCAGATGAGCACCCTTTCTTTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatgttcttggttctcaaatgcttctcatgatgactgcttttgtttctttgtcaccgtaagagatataaaccatcatttgcaatctaatgtacc...

Basic information

species	Zea mays
transcript	GRMZM2G028369_T01
intron #	3
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G028369_T01 (Zea mays), 5'ss of exon 3
lower sequence: LOC_Os01g55870.1 (Oryza sativa), 5'ss of exon 3

GTTGGGAGATACAAGAGCCCAGATGAGCACCCTTTCTTTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcat--------gttcttggttctcaaatgcttctcatgatgactgcttttgtttctttgtcaccgtaag-agatataaaccatcatttgcaatctaatgtacc
|||||||||||||||||||| ||||||||||| |||||||| |||||||||||||| || | |||||||||| | ||||| |||| ||||||||||        |||||| |   || | | ||||||   | |  | |     |   ||||| |   || | | | |||  || |||||  | |           
GTTGGGAGATACAAGAGCCCTGATGAGCACCCATTCTTTCCGGAGGATCTGCCTGAACCACTGTTGCCACCTCTCCAGTATCCAAAGgtaatgcataaactcgtgttcttagacatctagttcttctctgtaggctttcagcattccttttgttagtataggcaaacatagtccttcattgactaat---------

upper sequence: GRMZM2G028369_T01 (Zea mays), 5'ss of exon 3
lower sequence: GLYMA02G12580.1 (Glycine max), 5'ss of exon 2

GTTGGGAGATACAAGAGCCCAGATGAGCACCCTTTCTTTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatgttcttggttctcaaatgcttctcatgatgactgcttttgtttctttgtcaccgtaagagatataaaccatcatttgcaatctaatgtacc--
|| || |||||||||||||||||||||||  ||||||| ||||| ||  | || |||||   | | |||||| ||| ||| |    |||        || ||  ||||   ||     | | ||  |   ||||   ||    |||   | | |   | |  |||    |   | |  |||  |     
GTGGGAAGATACAAGAGCCCAGATGAGCATGCTTTCTTCCCTGAAGACTTACCCGAGCCAATGCTTCCACCTTTGCTgtaactgcaggt--------tttttaattct---atataagtgaggaacaa-actttacattacaagtcttagaatgtagttttgacctaactcaaccacataagttgattc

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211092233|gb|FL378442.1|FL378442

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGAT
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGAT

EST: gi|149067525|gb|EE157731.2|EE157731

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|71449541|gb|DR830591.1|DR830591

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|148987695|gb|EE031582.2|EE031582

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|91872761|gb|EB402718.1|EB402718

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|60337916|gb|DN204889.1|DN204889

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACNTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|78104614|gb|DV523032.1|DV523032

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|71447644|gb|DR828694.1|DR828694

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|71763787|gb|DR961724.1|DR961724

EST:     CTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: CTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

EST: gi|89764470|gb|DY691264.1|DY691264

EST:     TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGG                         GTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA
genomic: TTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 137

GTTGGGAGATACAAGAGCCCAGATGAGCACCCTTTCTTTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatg ... ctttgcatagGTTTTGCATCCCATTGCTGATTCTATCAATATCAACAAAGAGATTTGGAAAATGTATTTTGATGAACTTCTTCCAAGATTGGTGAAAAAAGGAAGTGATG

Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 206

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 235

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 394

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTTGGGAGATACAAGAGCCCAGATGAGCACCCTTTCTTTCCTGAGGATCTGCCTGAGCCCCGGTTGCCACCTATGCAGTACCCAAGGgtaatgcatgttcttggttctcaaatgcttctcatgatgactgcttttgtttctttgtcaccgtaagagatataaaccatcatttgcaatctaatgtacc

- - - - - - - - - - - - - - - - - - - - - GAGGAT