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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgggttcctgagctctctttcttttttttttcttaattcatgtcgcttcgtccttccgataacccacgggccacggccattcccag

Basic information

species Zea mays
transcript GRMZM2G082855_T03
intron # 24
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G082855_T02 (Zea mays), 5'ss of exon 23
lower sequence: LOC_Os06g03970.1 (Oryza sativa), 5'ss of exon 24
AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgggttcctgagctctctttcttttttttttcttaattcatgtcgcttcgtccttccgataacccacgggccacggccattcccag
||||| || || |||||||| ||||||||||| || || || || ||| |||| ||||| || |||||||| || || ||| |||||||||||||||| ||| |||    | |    | || |   || |   |  ||  | || |||   || ||  ||||   || | | |      | |  | ||  |||
AGGAATCTTGTTAGCCTCCATGGCTTCTCGCTATCTCCACATGGAAACTTGCTCTTCTATGATTACATGGAAAATGGTTCCTTGTGGGATCTTCTCCACGgttagg----gcttgaaacctttggctcatggataatttgttat-cattatgcatctccctt---atgatctata---tatgatccttttcag

upper sequence: GRMZM2G082855_T02 (Zea mays), 5'ss of exon 23
lower sequence: GLYMA09G27950.1 (Glycine max), 5'ss of exon 24
AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgggttcctgagctctctttcttttttttttcttaat-tcatgtcgcttcgtccttccgataacccacgggccacggccattcccag------
||||| || ||||   | || || |   | ||  | ||| | || || || || ||||| ||||| |||||||| ||||| |||||||||||||| |||||| |       |  |   |   |   || ||    ||| ||||| | || |    || |   | | ||||   |      |  |                
AGGAATCTTGTCACTTTGCATGGTTATGCTCTAACTCCTAATGGAAATCTTCTTTTCTATGACTATATGGAGAATGGCTCTCTGTGGGATCTTCTTCATGgtaatattcactcaccttgaaataactccttggaattttttaatgtgatatgaaatcttgtcttcaataataaaaaaaaaattgtagaaggtgcatcgaa

upper sequence: GRMZM2G082855_T02 (Zea mays), 5'ss of exon 23
lower sequence: GLYMA16G32830.1 (Glycine max), 5'ss of exon 25
AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtg-aggccgggttcctgagctctctttcttttttttttcttaat-tcatgtcgcttcgtcctt--ccgataacccac----gggccacggccattcccag
||||| || ||||   | || || |   | ||  | ||| | || || || || ||||| ||||| |||||||| ||||| |||||||||||||| ||||||  |  |      | |||     ||   | |   |||||    | | |||     || || |      | |||        ||  |||||  |||   | 
AGGAATCTTGTCACTTTGCATGGTTATGCTCTAACTCCTAATGGAAATCTTCTTTTCTATGACTATATGGAGAATGGCTCTCTGTGGGATCTTCTTCATGgtatattctctaaccttgaaacaactccttataaattttcactgtgttatgaaatctcatcttcaataaaaaacttgtatttggtgcacggaaattggaa-

upper sequence: GRMZM2G082855_T02 (Zea mays), 5'ss of exon 23
lower sequence: AT5G07180.1 (Arabidopsis thaliana), 5'ss of exon 25
AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgggttcctgagctctctttcttttttttttcttaattcatgtcgcttcgtccttccgata-acccacgggccacggccattcccag---
|| ||| | || ||| | ||||| | | |  | || ||    || ||||| || ||||||||||||||||| || ||||| || ||||||||||||||||||  |      ||| |   ||||    |   ||   |||||  ||||    | ||  | |||  |  | ||  |   |    |    |     |   
AGAAACATAGTAAGCTTGCACGGATACGCCTTATCTCCCTTTGGCAACCTCCTCTTCTACGACTACATGGAAAATGGCTCTCTTTGGGATCTTCTCCATGgtttgct---attcttcttctcttggaccagttacgttctt--ttcagccagttttctacttttggaagacttaaacgtagtgattgtatgatgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148957564|gb|EC903660.2|EC903660
EST:     GCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATG                         GCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAG
genomic: GCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAG
EST: gi|78117622|gb|DV536009.1|DV536009
EST:     GCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATG                         GCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAG
genomic: GCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAG
EST: gi|149088301|gb|EE177395.2|EE177395
EST:     GCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATG                         GCCCCGTCGA-GAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCA
genomic: GCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCC-GTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 909

AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAGTGGGCGCCGCGCAGGGGCTGGCGTACCTCCACCACGACTGCAACCCGCG


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 108

AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAGTGGGCGCCGCGCAGGGGCTGGCGTACCTCCACCACGACTGCAACCCGCG


Block sizes: 48 (upstream exon), 34 (downstream exon)
Score: 2

AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAGTGGGCGCCGCGCAGGGGCTGGCGTACCTCCACCACGACTGCAACCCGCG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 721

AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgg ... ccattcccagGCCCGTCGAAGAAGGTGAAGCTGGACTGGGACACGAGGCTGAGGATCGCAGTGGGCGCCGCGCAGGGGCTGGCGTACCTCCACCACGACTGCAACCCGCG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AGGAACCTCGTCAGCCTCCACGGCTTCTCGCTCTCCCCTCACGGGAACCTGCTGTTCTACGACTACATGGAGAACGGCTCCCTGTGGGATCTTCTCCATGgtgaggccgggttcctgagctctctttcttttttttttcttaattcatgtcgcttcgtccttccgataacccacgggccacggccattcccag

- - - - - - - - - - - - - - - - - - - - - - - - - GCTGTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CATGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGAA