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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtattaactggcagagcacatctatttgttcctttttcgttctatacgctattaagtgaaaagtcaatctcttccttttgctag

Basic information

species Zea mays
transcript GRMZM2G060045_T01
intron # 22
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G060045_T01 (Zea mays), 5'ss of exon 22
lower sequence: LOC_Os05g01700.1 (Oryza sativa), 5'ss of exon 20
GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtattaact--ggcagagcacatctatt----tgttcctttttcgttctatacgctattaagtgaaaagtcaatctcttccttttgctag
|||||||||| |||| |||||||||||||| ||||| ||||| || ||  ||||||||||||||||| | ||||| || ||||| ||||||||||| |||||| || | ||||  |    ||    |  |||    ||  |  ||  || |  |  | || || | ||||     |    ||   || |   ||
GAATGTTCGGTTGGTCTATCTTCTAGAAAGAGAAGGATGGGACTCAACTTCAAACTGGGAAGATGTTTTATCGTTGGGAGAACAGCAGAGGCTTGGGATGgtcagtgtaaactctgtttcagtctgttaattgcgatgaccagttgccgcttaaatccctttttattgaagttggagctgctgttttcttgcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60345941|gb|DN212914.1|DN212914
EST:     CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATG                         GCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
genomic: CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
EST: gi|60351696|gb|DN218669.1|DN218669
EST:     CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATG                         GCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
genomic: CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
EST: gi|60342685|gb|DN209658.1|DN209658
EST:     CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATG                         GCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
genomic: CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
EST: gi|32826847|gb|CD966525.1|CD966525
EST:     CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATG                         GCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
genomic: CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
EST: gi|211295643|gb|FL223363.1|FL223363
EST:     CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATG                         GCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
genomic: CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
EST: gi|76292548|gb|DV032116.1|DV032116
EST:     CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATG                         GCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC
genomic: CAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 54

GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACCAA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 56

GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACCAA


Block sizes: 47 (upstream exon), 45 (downstream exon)
Score: 20

GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACCAA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 114

GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtatta ... cttttgctagGCTCGTTTGTTTTTCCATCATCCTAAGTTTGGTATCCTGGACGAGTGCACCAA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GAATGTTCGGCTGGTGTATCTTCTAGAAAGGGAAGGGTGGGATTCTACACCAAACTGGGAAGATGTTCTGTCGTTAGGGGAACAACAGAGGCTTGGAATGgtcggtattaactggcagagcacatctatttgttcctttttcgttctatacgctattaagtgaaaagtcaatctcttccttttgctag

- - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG