ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GGTGGTCTTCTGTATATTTGTGGCCAGACATCTTATCAATGACTTTCCTGATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttgttgtgctttctgaacttattcaagtaattttgttgaagcttttctgcatttggttctttcatatgatcatatggcagttaaaccacgttc...

Basic information

species	Zea mays
transcript	GRMZM5G868588_T01
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G868588_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os09g33450.2 (Oryza sativa), 5'ss of exon 3

GGTGGTCTTCTGTATATTTGTGGCCAGACATCTTATCAATGACTTTCCTGATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttgttgtgctttctgaacttattcaagtaattttgttgaagcttttctgcatttgg--ttctttcatatgatcatatggcagttaaaccacgttc
  | |||||||||||||||||||| |||||||| ||||||||||| |||||||| ||||||||||||||||||||| ||||| | ||||||||  |||| |||||| ||| |    || |||    |  |    |  | |||||    ||    ||  |   |  |   ||  |      |    | | ||    ||  |  
ATTAGTCTTCTGTATATTTGTGGCTAGACATCTGATCAATGACTTCCCTGATCACTGGGGAGTTTCAGTTATGGTCGTAGTGGTCGTCTTCACAATATATgtaagtattgcta-atttgctgcttctcatttttttttcttgttccctctaaattgattgaaggctgtcttagttctgctactctgaatttggttcatatg-

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211226399|gb|FL202748.1|FL202748

EST:     ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATAC                         GATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT
genomic: ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT

EST: gi|211226401|gb|FL202750.1|FL202750

EST:     ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATAC                         GATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT
genomic: ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT

EST: gi|211226408|gb|FL202757.1|FL202757

EST:     ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATAC                         GATTTAACATTGCTGCTTCTTACCTCTG
genomic: ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTG

EST: gi|148970678|gb|EE021391.2|EE021391

EST:     ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATAC                         GATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT
genomic: ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT

EST: gi|88747254|gb|DY531395.1|DY531395

EST:     ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATAC                         GATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT
genomic: ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT

EST: gi|76013624|gb|DT940794.1|DT940794

EST:     ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATAC                         GATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT
genomic: ATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 162

GGTGGTCTTCTGTATATTTGTGGCCAGACATCTTATCAATGACTTTCCTGATCATTGGGGAGTTTCAGTTATGGTCATAGTGATTGTCTTCACTGTATACgtaagtgttg ... tgttttccagGATTTAACATTGCTGCTTCTTACCTCTGGTAGAGATCCTGGTATAGTGCCTCGTAACACCCATCCTCCTGAAACTGACGCTATTGAAATGAACAATGATG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 112

Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 77