ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg...

Basic information

species	Zea mays
transcript	GRMZM2G468260_T02
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os11g41140.1 (Oryza sativa), 5'ss of exon 3

ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg----
||| |||| || ||| ||||| ||| ||||| ||||||||| ||||  || ||||||||||| |||||||||||||||||||| ||||||||||||||||||||||    | |  ||||   | |  ||  | ||   |    || |  | | | || |    ||  |      || |||    | |  | |    | ||    
ACTCATGGAGAGAAGCTGCCAACAAAGGAACCTGGAAATTATTTCACATACTTTTCCCCATCCGCTGAAGATGAAGATGTCTGCCCAACTTGTCTTGAAGgtattaatctacttgtcatcagtata-ctagaatgacatatgtagttaatattacatatgc--aattgtcttcatagaatacttaatg-agagtcatgtgtact

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA18G50950.1 (Glycine max), 5'ss of exon 3

ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacg----ctacagt-aaaatttccattacataagactttg--
   |    ||  | | |  || ||    ||| |  || |       |   | | |   ||||  | ||||| || ||||||||||||||||||||||||||||  |  |      |  | | ||| | ||  |   |||  | || |||| |    | |  ||  | |     || | | ||||||     ||||    |  |    
-AGTCCACTGTAAGGCTCTCATCAGCAAAACTTACAACTGGAGCTGGGGTTGTCTATTCATCATCAGAAGAGGAGGATGTCTGTCCAACTTGTCTTGAAGGtaagagtg------ttttctctttttttttggctaatttcagagctgctatagcctatagcaggatggctgttatactgaaaattatggatacaagctaaataact

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA08G27730.1 (Glycine max), 5'ss of exon 3

ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg---------
|  |    ||  |   |  || ||    ||| |  || |       |   | | |   ||||  | ||||| || ||||||||||||||||||||||||||||        | | |  ||||| | | ||  |   |||  | |  || | |   |   |   |  |   |  | |  || | |  ||  |||| | |||         
AAGTCCTCTGTAAGACTCTCATCAGCAAAACTTACAACTGGAGCTGGGGTTGTCTATTCATCCTCAGAAGAGGAGGATGTCTGTCCAACTTGTCTTGAAGgta--------agagtgttttttctttttttggccaatttcagaactgttgtaggaggatgttaggcaaga-ggaatcatctactgtagctaagccgttgctacggcac

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA13G06840.1 (Glycine max), 5'ss of exon 2

-ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg---
 | |  |    | |   | | | |||   || ||  || |    |  |   | | |   ||||  | || || || ||||||||||||||||||||||||||||       |   ||  ||||||| ||||||   | ||  |           | |    |  | || |   ||| | |||| ||  |  ||    | ||   
AAGTCCTCACTA-AGACTTCAATCAACAAAATATTCAACTGGAGTTGGTCTTGTCTATGCATCATCAGAGGAGGAGGATGTCTGTCCAACTTGTCTTGAAGgta--gatatatatatattttttttatcttaataatcttatattttctacagaagacccattgatac-caggagttacattttcaggaagtattgatctgtta

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: AT5G15790.2 (Arabidopsis thaliana), 5'ss of exon 3

ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTT-CAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg---
 | | | | ||  |     ||   | |||  |||   | || |  |       | |    | |  |||| |||||||||||||||||||| ||||||||||||||  ||    | ||| ||||||| ||||   | | |||||   ||||      ||||   | ||   ||   | | |||   ||| | |  |  ||||   
TCCTCTCG-GAGGATTCTTCAGTCAAGGACAATGTCTACTAGTAACGAAGGCCTGTATATAACATCTGATGATGAAGATGTCTGTCCAACGTGTCTTGAAGgtat--tcattttcatc-tttttttttctt---tttcttggatcaatgc------atttcaaagaagcttagtatcagatt---atttcgtgtg-ttttgtag

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: AT3G02290.1 (Arabidopsis thaliana), 5'ss of exon 3

ACTTATGGTGATAAGGTGCCA--CCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtatta-tcgcaataatcatttttttctcttaagtgtattggaaag--atgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg
 || | | |  | || |      |||||  || |  |     |||      |  | |    ||   ||||||| ||||||||||| ||||||||||||||||||| || |      | |||    | ||  ||  ||   ||    |   ||    || |  ||||    |                                  
-CTCAGGATTTTGAGATCAAGGTCCAAGTCAATAATGGC-CGACTCTGAAAACATGTATATATTGTCTGAAGACGAAGATGTCTGCCCAACTTGTCTTGAAGgtaataataatcccattcaacagtctcctttttgttccttcagtattcatatcttgttggttttttgcag---------------------------------

upper sequence: GRMZM2G468260_T02 (Zea mays), 5'ss of exon 2
lower sequence: Vv14s0083g01000.t01 (Vitis vinifera), 5'ss of exon 3

--ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg----
         |||| |   | | |       ||||   |    ||||  | | | |||   ||||  | |||||||| |||||||||||||| |||||||||||||  |    |  |   |||||  | | |    ||||     || |    || |  | |   | |||  |    |  ||   |   ||    |  || |     
AGGTCCTCAGTGA-AGCATTCGATGGCAAAAACAACAATGGGACTTGGGTT-TATTTATTCATCGTCAGAAGATGAGGATGTCTGTCCAACATGTCTTGAAGgtaaaagtttataatatattttcctttttatgttgtagcaataaaa----ctaaactatgctataaccttgatatgcaaacacagctaacccatgctgtaacct

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|149029755|gb|EE154767.2|EE154767

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|67028741|gb|CO457490.1|CO457490

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTTTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|8103125|gb|AW927785.1|AW927785

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTTTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|18179901|gb|BM381111.1|BM381111

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|78178991|gb|DV549364.1|DV549364

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTTTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|93012750|gb|EB638270.1|EB638270

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAG                         ATTATGATTTTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|211295922|gb|FL460683.1|FL460683

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTTTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|211131422|gb|FK982405.1|FK982405

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTTTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|50337985|gb|CO533111.1|CO533111

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|7844561|gb|AW787783.1|AW787783

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTTTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|71756382|gb|DR954319.1|DR954319

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

EST: gi|71444055|gb|DR825105.1|DR825105

EST:     TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAG                         ATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC
genomic: TTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 41 (upstream exon), 46 (downstream exon)
Score: 76

ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgc ... taaattgcagATTATGATTCTGAGAATCCTAGGATAGTAATGCAGTGCTCACACCATTTCCACCTTGGCTGTATTTATGAGTGGATGGAAAGAAGTGAGGCATGCCCTGT

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 190

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 220

Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 147

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ACTTATGGTGATAAGGTGCCACCAAGGGAACATGGAAATTACTTCAGCTATTTTTCCCCATCTGCTGAAGATGAAGATGTCTGTCCAACTTGTCTTGAAGgtattatcgcaataatcatttttttctcttaagtgtattggaaagatgctctgatatttttcaaaacgctacagtaaaatttccattacataagactttg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG