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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctttggtctaatttcattcattcatttcgattgggtggtgagttggggtgtggctgttgtggatgcag

Basic information

species Zea mays
transcript GRMZM2G304362_T01
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G304362_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os06g07210.1 (Oryza sativa), 5'ss of exon 2
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctttggtctaatttcattcattca---------------tttcgattgggtggtgagttggggtgtggctgttgtggatgcag
|| |||||||||||||| || || ||||||||||||||||| |||||||||||||||||||||||||  |  | | ||||| || | | || | |||| |                | | |||| ||  |||| ||||| | |||  ||| |||||||||
CTCGCGGCGAGGATTGCGGTTTCCAACCTGCACAAGAACACCAAGAAGTCCTTCTCGGAGACgtaagcgattgattgcttttgtttcagttgaatcatgcgaagggtgttgggatttatggattaggatgtgaattgggatttggtcgttatggatgcag

upper sequence: GRMZM2G304362_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA06G15350.1 (Glycine max), 5'ss of exon 2
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctttggtctaatttcattcattcatttcgattggg------tggtg-agttggggtgtgg--ctgttgtggatgcag
 |||| || |||||||| || ||||| |||||||||||||| |||||||| ||||||||||||| |||       |   |||   |    |  || |||  |||   || |       | |||  |||      |||   ||| |||||| |||
TTGGCTGCTAGGATTGCCGTTTCGAATCTGCACAAGAACACCAAGAAGTCGTTCTCGGAGACgtgagtgtgaatggaaattgtaattgcgtttttaatttgtttttgttagcgttattttgtgtggttaaaacttggaattgtggtggattcag

upper sequence: GRMZM2G304362_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA04G39500.1 (Glycine max), 5'ss of exon 2
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctt--tggtctaatttcattcattcatttcgattgg-gtggtgagttgggg--------tgtggctgttgtggatgcag
 |||| || |||||||| || ||||| |||||||||||||| ||||| |||||||| ||||||| |||       |   |  |  |    |||  || |||  |||   || | || ||    || ||        ||    ||| |||||| |||
TTGGCTGCTAGGATTGCCGTTTCGAATCTGCACAAGAACACCAAGAAATCCTTCTCCGAGACgtgagtgtgaatggaaatgataattgcgttttcttaatttgtttttgttagcgttgttttgtgaggcttaaacttgaaattgtggtggattcag

upper sequence: GRMZM2G304362_T01 (Zea mays), 5'ss of exon 2
lower sequence: AT2G21790.1 (Arabidopsis thaliana), 5'ss of exon 2
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacgg---ctggctttg---gtctaatttcattcat-tcatttcgattgggtg-gtgagttggggt--gtggctgttgtgga----tgcag
|| || || ||||||||||||||||| || ||||||||||| |||||||| || || ||||||| |||   |    ||  ||| |   || | ||  | || || | | ||| | |      ||| ||||       |||||  |||||     |||||
CTTGCTGCTAGGATTGCTGTGTCGAATCTCCACAAGAACACTAAGAAGTCATTTTCTGAGACgtgagtgttgagttctttcttagtgtgtattatacccttgatatgagttcaagtttccatgtgtgttgactccgatggcttgtgtggtatcttgcag

upper sequence: GRMZM2G304362_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ14283 (Selaginella moellendorffii), 5'ss of exon 2
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctttggtctaatttcattcattcatttcgattgggtggtgagttggggtgtggctgttgtggatgcag
|| || || ||||||| ||| ||||| || |||||||| || || ||||||||||| |||||||||||      |   |   |  |   |  |||   | |       ||||   | ||                          
CTTGCTGCCAGGATTGTTGTCTCGAATCTTCACAAGAAAACTAAAAAGTCCTTCTCAGAGACgtaagt-----tttcttcgagaatttctctattgtctaaccaaagctgggctatcag--------------------------

upper sequence: GRMZM2G304362_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ20157 (Selaginella moellendorffii), 5'ss of exon 2
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctttggtctaatttcattcattcatttcga-ttgggtggtgagttggggtgtggctgttgtggatgcag
|| || || ||||||| ||| ||||| || |||||||| || || ||||||||||| |||||||||||      |  ||| |      | ||  |  |  |     |  ||||   | ||                          
CTTGCTGCCAGGATTGTTGTCTCGAATCTTCACAAGAAAACTAAAAAGTCCTTCTCAGAGACgtaagt------tttcttcgagaagttctctattgtctaaccaaagctgggctatcag--------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|93012077|gb|EB637597.1|EB637597
EST:     ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGAC                         GATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
genomic: ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
EST: gi|31355936|gb|CD440293.1|CD440293
EST:     ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCATTCTCGGAGAC                         GATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
genomic: ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
EST: gi|88756462|gb|DY540603.1|DY540603
EST:     ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGAC                         GATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
genomic: ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
EST: gi|31355336|gb|CD439693.1|CD439693
EST:     ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCATTCTCGGAGAC                         GATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC
genomic: ATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 294

CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCCTCTTGTCGCTGAAGATGTTTATGAAATCATCATGAAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 59

CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCCTCTTGTCGCTGAAGATGTTTATGAAATCATCATGAAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 26

CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCCTCTTGTCGCTGAAGATGTTTATGAAATCATCATGAAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 79

CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacg ... gtggatgcagGATTAAGGACATGTACATGCACTTCAACAAGAGATCCGGGCTGATGGCCCCTCTTGTCGCTGAAGATGTTTATGAAATCATCATGAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CTGGCGGCGAGGATTGCTGTGTCGAACCTGCACAAGAACACAAAGAAGTCCTTCTCGGAGACgtaagtaacggctggctttggtctaatttcattcattcatttcgattgggtggtgagttggggtgtggctgttgtggatgcag

- - - - - - - - - - - - - - - -  AAGAAC
- - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - -  AGAAGT