ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatcctctggtctttattgcatctatgccttaagaattgttcctgttgtgtttacatctaatgaacattttagaatgtaagtcgaaggagttggttctaacacgttgtgatgtatgcatcttgcag

Basic information

species	Zea mays
transcript	GRMZM2G181194_T02
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G181194_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os08g44380.2 (Oryza sativa), 5'ss of exon 5

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatcctctggtctttattgcatctatgccttaagaattgttcctgttgtgtttacatctaatgaacattttagaatgtaagtcgaaggagttggt--------tctaacacgttgtgatgtatgcatcttgcag
 || || |||||| |||| |||||||||||||||||||| || |||||||| |||||| |||||||||||||||| ||| ||||||||||||||||||||||||||  |  |||   ||| || |||||  |   |||       || |||   ||||||||| |||||||  || || | |||||| | ||  ||             ||||||  ||| || | | |||   ||||||
TGCTGTGGGTAACTTGTCGATGGAGGAGAAGCAGATCCAGCAAAACATCCAAATGAGCGTCAACTTCCTCGTGTCCCTTTTGAAGAAGAACTGGCAGAATgtgagttctgttctcaacttcatggcatcagtagttta-----catttctg--atgtttacatgtaatgaaatttatatagtgtaagcccaacaagcaaacacaatcagtctaacttgttatggtttgtgc--tttgcag

upper sequence: GRMZM2G181194_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os08g44380.1 (Oryza sativa), 5'ss of exon 5

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatcctctggtctttattgcatctatgccttaagaattgttcctgttgtgtttacatctaatgaacattttagaatgtaagtcgaaggagttggt--------tctaacacgttgtgatgtatgcatcttgcag-----
    || |||||| |||| |||||||||||||||||||| || |||||||| |||||| |||||||||||||||| ||| ||||||||||||||||||||||||||  |  |||   ||| || |||||  |   |||       || |||   ||||||||| |||||||  || || | |||||| | ||  ||             ||||||  ||| || | | |||   ||||||     
----GTGGGTAACTTGTCGATGGAGGAGAAGCAGATCCAGCAAAACATCCAAATGAGCGTCAACTTCCTCGTGTCCCTTTTGAAGAAGAACTGGCAGAATGTGAgttctgttctcaacttcatggcatcagtagttta-----catttctg--atgtttacatgtaatgaaatttatatagtgtaagcccaacaagcaaacacaatcagtctaacttgttatggtttgtgc--tttgcaggtgag

upper sequence: GRMZM2G181194_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os08g44450.1 (Oryza sativa), 5'ss of exon 4

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatcc--tctggtctttattgcatctat---gccttaagaattgttcctgtt----------gtgtttacatctaa----tgaacat-tttagaatgtaagtc------gaaggagttggttctaacacgttgtgat-gtatgcatcttgcag-----
    || |||||| |||| |||||||||||||||||||| || |||||||| |||||| |||||||||||||||| ||| |||| ||||||||||||||||||||| |||   ||||  | |  ||||||  |      ||||    |     |||||          |||  || || | |    ||  | |  || ||||   |||       ||   |     |  | | |  |||  || || ||  | |   ||     
----GTGGGTAACTTGTCAATGGAGGAGAAGCAGATCCAGCAAAACATCCAAATGAGCGTCAACTTCCTCGTGTCCCTTTTGAAAAAGAACTGGCAGAATGTGAgttatctgttctgagcctgtttgcatgcaattaaacttacagctcaaatctgtttaacaacatagtgaatatatatgagttttggccttgctttgaataatagttttatgtgagaaaacaactgatgataacttggaattgtttgtgtttgataggtgag

upper sequence: GRMZM2G181194_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os08g44450.3 (Oryza sativa), 5'ss of exon 2

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc--ctctggtctttattgcatctatgc---cttaagaattgttcctgtt----------gtgtttacatctaa----tgaacat-tttagaatgtaagtc------gaaggagttggttctaacacgttgtgat-gtatgcatcttgcag
 || || |||||| |||| |||||||||||||||||||| || |||||||| |||||| |||||||||||||||| ||| |||| ||||||||||||||||||||| |||   ||||  | |  ||||||  |      ||||    |     |||||          |||  || || | |    ||  | |  || ||||   |||       ||   |     |  | | |  |||  || || ||  | |   ||
TGCTGTGGGTAACTTGTCAATGGAGGAGAAGCAGATCCAGCAAAACATCCAAATGAGCGTCAACTTCCTCGTGTCCCTTTTGAAAAAGAACTGGCAGAATgtgagttatctgttctgagcctgtttgcatgcaattaaacttacagctcaaatctgtttaacaacatagtgaatatatatgagttttggccttgctttgaataatagttttatgtgagaaaacaactgatgataacttggaattgtttgtgtttgatag

upper sequence: GRMZM2G181194_T02 (Zea mays), 5'ss of exon 2
lower sequence: PP1S40_297V6.1 (Physcomitrella patens), 5'ss of exon 3

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtg-atcctctggtctttatt-gcatcta-tgccttaagaattgttcctgttgtgtttacatctaatgaacattttagaatgtaagtcgaaggagttggttctaacacgttgt--gatgtatgcatctt-gcag---
|||  ||||||||     ||||||||| ||||| |||   |||||||  || ||| |  | |||||||||||||| ||  |||||||||||||||||||||| |||  ||    |  | | |||| | ||  | |   |    |  ||||| |||  || ||  || |  | |  |||     |||    |  || |  |  |||| |  |  || |   | || || || || | |    
CGCCATCGGTAACTGTGATATGGAGGAAAAGCAAATCTTCCAGAACACACAAATGGGTGTTAACTTCCTCGTGTCCCTGTTGAAGAAGAACTGGCAGAATgtaagtacatttgttcctttctatttgaatgcagtatatctgaaggtgttcttgtgatgcttgtattt--tcattattaagtgatggtcatacaatgtctaagttc-agtattttatctaacgtttgaatttttgtaacag

upper sequence: GRMZM2G181194_T02 (Zea mays), 5'ss of exon 2
lower sequence: PP1S18_121V6.1 (Physcomitrella patens), 5'ss of exon 3

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatcctctggtc---tttattgcatctatgccttaagaattgttcctgttgt-gtttacatctaatgaacattt--tagaatgtaagtcgaaggagttggttctaa-cacgttgtgatgtatgcatcttgcag---
 ||  | || ||       ||||||||||||||||||   || ||||  |||||| |  |||||||||| || || || ||||||||||||||||||||||| ||  |    ||       || ||   || |  | | |     || |    ||  | ||||  ||  | |    ||||  | ||   |  ||||  |        |||||  |   |||      |   |||||  |   
TGCCATTGGAAATTGTGACATGGAGGAGAAGCAGATCTTCCAAAACACTCAGATGGGTGTCAACTTCCTTGTATCCCTGCTGAAGAAGAACTGGCAGAATgtaagcaacaaactcaatagtttcatcatatttccgacctgttccttttattcgtattagtttgtattaattcggtatttactcgagcttgcgtcgctgatcacatctctaagtattctgttcatactttttcttgtggcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|5713971|gb|AI943956.1|AI943956

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|32943776|gb|CF048595.1|CF048595

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|32841275|gb|CD980956.1|CD980956

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|32930778|gb|CF035590.1|CF035590

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|60347511|gb|DN214484.1|DN214484

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|211379030|gb|FL141076.1|FL141076

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|32841237|gb|CD980918.1|CD980918

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|19769382|gb|BQ034103.1|BQ034103

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|6020458|gb|AW065386.1|AW065386

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|211268446|gb|FL141046.1|FL141046

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGATCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|60358826|gb|DN225799.1|DN225799

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|166474714|gb|EG122102.1|EG122102

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTC

EST: gi|84977553|gb|DW475975.1|DW475975

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|15323558|gb|BI478780.1|BI478780

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|211263568|gb|FL141014.1|FL141014

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|6865330|gb|AW360680.1|AW360680

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|60345300|gb|DN212273.1|DN212273

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|60357767|gb|DN224740.1|DN224740

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|60396150|gb|DN229019.1|DN229019

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|32934673|gb|CF039485.1|CF039485

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|33093601|gb|CF053595.1|CF053595

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|32930672|gb|CF035484.1|CF035484

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|211242318|gb|FL469539.1|FL469539

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

EST: gi|5018419|gb|AI714612.1|AI714612

EST:     AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAAT                         GTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC
genomic: AGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 465

CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatc ... catcttgcagGTAAGATGTCTCTACATCAAGAGCACCATGGGGAAACCATTCAGGATTTTCTAAGTCTGTTTTTTTGTGAAGTCTGCTCTTTATGATATTTGGTTTTTGA

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 261

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 347

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 193

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CGCGGTCGGTAACCTGTCTATGGAGGAGAAGCAGATCCAACAGAACATCCAGATGAGCATCAACTTCCTCGTGTCTCTTCTGAAGAAGAACTGGCAGAATgtgagtgatcctctggtctttattgcatctatgccttaagaattgttcctgttgtgtttacatctaatgaacattttagaatgtaagtcgaaggagttggttctaacacgttgtgatgtatgcatcttgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAC