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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgcatgacatgttcatggcatttttatgtcagatcttaaatgcgtttgagtgttttgcttgtctgcag

Basic information

species Zea mays
transcript GRMZM2G167668_T01
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G167668_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os01g72310.1 (Oryza sativa), 5'ss of exon 7
TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgt-gcatgacatg--ttcatggcatttttatgtcagatcttaaatgcgtttgagtgttttgcttgtctgcag
||||||||| |||||||| ||||| ||||| ||||| |||  ||| || |||||||| |||||||||||||| |||||||||||| | || ||||| |||||  || | | || |||    ||| |  ||||    ||| |  |||||  |   ||||      || ||   |  |||
TATTAGTCGTGCACATCGTATGGGTGCTACTCGTCCCATATTTGTTGAGACTTTGGCCATGCGTGGTACCATCGAAGAGCAAATGTTAAAACTTTTACAGgtacgttttggatcacaccgtttcgtaacattccactgtgaagtcttaccta--tttg-----ctttctccaccacag

upper sequence: GRMZM2G167668_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA04G28970.2 (Glycine max), 5'ss of exon 7
TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgcatgacatg------ttcatggcatttt---tatgtca-----gatcttaaatgcgtttgagtgttttgcttgtctgcag-----------------------------------------------------------------------------------------------------------
 |||||||| || ||||||||||| ||| | ||||| || ||||| ||||| || || ||||||||||| |||||||||||||||||     |||| |||||   | |  || |  ||      || ||  |  |||   | |   |      ||  |   |   ||||| ||  ||| |||| |                                                                                                               
AATTAGTCGTGCTCATCGAATGGGTGCTTCTCGTCCTATTCATGTGGAAACATTAGCAATGCGTGGTACAATTGAAGAGCAAATGCTAGGCTTTTTACAGgtaaatctctataaattgaactattttatacctatttgagtgtactactaacaatgatgggtctctttgaatgccttgtttgtttatgttgccattcatgtatctcactgaaaattgtctcctcaatcccttttgtttgtttagctcttatattattgttgttgttgttgttcatttttcaatttgttgatgccag

upper sequence: GRMZM2G167668_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA11G21600.1 (Glycine max), 5'ss of exon 7
TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgcatgacatgttc-atggcatttttatgtcag-------------atcttaaatgcgtttgagtgttttgcttgtctgcag------------------------------------------------------------------------------------------------------------
 |||||||| || ||||| ||||| ||| | ||||| ||  |||| ||||| || || ||||||||||| |||||||||||||||||  |  |||| |||||   | |  || |  ||  | ||   |    ||| | ||             ||  |   |   |||||  |  ||| | || |                                                                                                                
AATTAGTCGTGCTCATCGTATGGGTGCTTCTCGTCCTATTTATGTGGAAACATTAGCAATGCGTGGTACAATTGAAGAGCAAATGCTAGACTTTTTACAGgtaaatctctataaattgaactatcttacacctatttgagtgtactactaacaatgatgggtctctttgaaggccttgttagtttatgttgtcgttcatgtatgtcactgaaaattgtctcctcaatgtcttttgttttgtttagcttttatattgttgttgttgttcatctttcaatttgttgatggtttaagcag

upper sequence: GRMZM2G167668_T01 (Zea mays), 5'ss of exon 2
lower sequence: Vv13s0067g03330.t01 (Vitis vinifera), 5'ss of exon 8
TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttg-----tgtgcatgacatgttcatggcatttttatgtcagatcttaaatgcgtttgag---tgttttgcttgtc-tgcag-----------------
 || ||||| || || || ||||| ||||| || || || | ||| |||||||| || ||||||||||| || ||||||||||||||| |  |||| ||||| ||     ||    ||||||||| || ||||| |||      | |   |||  |    ||   ||||||  || || |  |                  
GATCAGTCGTGCCCACCGGATGGGTGCTACTCGGCCTATTCGTGTGGAAACTTTAGCAATGCGTGGTACAATCGAAGAGCAAATGCTTGAATTTTTACAGgtatggcttttggtactgacatgtt-attgcattattaccatgaagcactaataagagaaagacctgttttttttttcctatactcagttgtcttgttgac

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|8401373|gb|BE057007.1|BE057007
EST:     CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAG                         GATTCTAACGCCTGCAGAAAAATAGTGGATAAAGGAACAGGCAGCACTGAC
genomic: CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGAC
EST: gi|32936690|gb|CF041509.1|CF041509
EST:     GGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAG                         GATTCTAACGCCTGCAGAAAAATAGTGGATAAAGGAACAGGCAGCACTGAC
genomic: GGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGAC
EST: gi|6056569|gb|AW090974.1|AW090974
EST:     CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAG                         GATTCTAACGCCTGCAGAAAAATAGTGGATAAAGGAACAGGCAGCACTGAC
genomic: CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGAC
EST: gi|113641316|gb|DW804488.1|DW804488
EST:     CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAG                         GATTCTAACGCCTGCAGAAAAATAGTGGA
genomic: CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGA
EST: gi|5901375|gb|AW042475.1|AW042475
EST:     CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAG                         GATTCTAACGCCTGCAGAAAAATAGTGGATAAAGGAACAGGCAGCNCTGAC
genomic: CTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGAC
EST: gi|32931114|gb|CF035926.1|CF035926
EST:     ACCATTGAAGAGCAAATGCTTAAGCTTTTGCAG                         GATTCTAACGCCTGCAGAAAAATAGTGGATAAAGGAACAGGCAGCACTGAC
genomic: ACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 55

TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGACAATGAAGGAGGGCGGTCCCATCGGAGCCTCCATGATTTTGCTGAAAGCA


Block sizes: 43 (upstream exon), 46 (downstream exon)
Score: 40

TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGACAATGAAGGAGGGCGGTCCCATCGGAGCCTCCATGATTTTGCTGAAAGCA


Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 48

TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGACAATGAAGGAGGGCGGTCCCATCGGAGCCTCCATGATTTTGCTGAAAGCA


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 91

TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgc ... ttgtctgcagGATTCTAACGCCTGCAGAAAAATAGTGGATAAAAGAACAGGCAGCACTGACAATGAAGGAGGGCGGTCCCATCGGAGCCTCCATGATTTTGCTGAAAGCA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TATTAGTCGGGCACATCGAATGGGGGCTACGCGTCCAATACATGTAGAAACTTTGGCTATGCGTGGTACCATTGAAGAGCAAATGCTTAAGCTTTTGCAGgtttgtgtgcatgacatgttcatggcatttttatgtcagatcttaaatgcgtttgagtgttttgcttgtctgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCA