ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgtgatggtgaactaatagttatgcgtaattgtttgcag

Basic information

species	Zea mays
transcript	GRMZM2G145308_T01
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os03g08440.1 (Oryza sativa), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgtgatggtgaactaatagttatgcgtaattgtttgcag
 ||||| ||||| || |||||||| || |||||||||||||||||||||||||| ||||| || ||||||||||| |||||||||||||| || ||||||||||| |  |      | |||  ||        | |   |   |  | ||||| | | |||||  |  ||| |   |  |  ||  | || || |||||
TGCTGGAAGGCATACGCCTGGTACATTTACCAATCAGCTCCAGACCTCCTTCAGCGAGCCCCGTCTGCTCATCCTCACTGACCCAAGGACCGATCACCAGgtgaaaatgatcaccatgtcacggt--------agtgcgttggtgaattgggtacagtattgtt-tactgatcgtgttatcctgaattat-gtctgcag

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA16G01460.1 (Glycine max), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagaga-agttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattg--tgatggtgaac-taata---gttatgcgtaattgtttgcag-----------------------------------------------------------
 ||||| |||||||| || |||||||||||||||||||| ||||| || |||||||||||||| || || ||||| ||||| ||||| || || ||||||||| | |   |  |  | ||||||        || |||| ||  || |||   |  ||| |||  |||| ||  || | ||    ||||||  |  |||||||| |                                                           
TGCTGGAAGGCACACTCCCGGTACCTTCACCAATCAGCTTCAGACTTCGTTCAGTGAGCCTCGTCTCCTTATCCTCACTGATCCAAGAACAGATCACCAGgtggatacccaaatcagcttcaaat-------gatatatttgattttagttacttgtgctttgattgattgttgacatgatttatgttatgtatg-ttgtttgcggttgttgtggtgttgactgtgttgtgttgttaatgtgtgtgtttgttcgtttgtgtgcag

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA07G04890.1 (Glycine max), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaa-gttttgtttcaaagttctt----aagaaatatatgcattgagtgggtgctgcat-tgtgatggtgaactaatagt------tatg-cgtaattgtttgcag----------------------------------------------------------
 ||||| |||||||| ||||||||||||||||||||||| || || |||||||| |||||||| || || || ||||| || ||||| || || |||||||| |  |     ||||  |    |||| |      | ||| | || ||||   |    |   ||| ||   || |  | |  |  |      ||||  || ||||||||                                                             
TGCTGGAAGGCACACTCCTGGTACCTTCACCAATCAGCTTCAAACTTCCTTCAGCGAGCCTCGTCTCCTTATTCTTACCGATCCAAGAACAGATCACCAGgttagtaccctattttcatatgcagttatatgaagaaaaaaagatacatttgattctagtcacatgtggtttgattgattgttgatgtagtttatggtgtgattgtttgtttagggttgttgtattgcccgtgttgtgttgttaatgtatgtttgttggtttgtgtgcag

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: AT1G72370.2 (Arabidopsis thaliana), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgtgatggtgaactaatagttatgcgtaattgtttgcag
 ||||| || ||||| ||||||||||||||||||||| | ||||| ||||||||||| ||  | ||| | || |||||||||||||| |||||||||||||| ||        |  |   |   |||       |||||   | ||| ||  |  ||  || || |  |||  |||  ||  || ||   |||| ||||
TGCTGGAAGACACACTCCTGGTACCTTCACCAATCAGATGCAGACATCCTTCAGTGAACCAAGGCTGTTGATTCTTACTGACCCAAGAACTGACCACCAGgtaaa-------ctcttcatatgattc-------atatac-tagtgattgattaatgatttttgttattga--taaatgtcttgtgtg--tgtt-gcag

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: Vv09s0002g00610.t01 (Vitis vinifera), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgt---gatggtgaactaatagttatgcgtaattgtttgcag----
 ||||| |||||||| || ||||| |||||||||||||||||||| || ||||||||||| || || |||||||| || |||||||| ||||| |||||||| ||| |||    |    || | |  |||  || | | |   | |  |||||  |    |     || ||||     |    | |   ||          |    
TGCTGGAAGGCACACACCAGGTACATTCACCAATCAGCTCCAGACATCTTTCAGTGAGCCCCGTCTTCTCATCCTCACAGACCCAAGAACTGATCACCAGgt-aagtgaa----taacacaca-tctttataaattctgtttttagtttgggttttagggttcaaggaaggtgcgtagactactttttctagccaaccagggaaat

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: PP1S59_133V6.1 (Physcomitrella patens), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgtgatggtgaactaa-tagttatgcgtaattgtttgcag------
 |||||||| |||||||| || || |||||||||||||| |||| | ||||||| |||||  | || || ||  | || || ||  | ||||| |||||||| | |     |||||  |   | | ||        ||   ||| | || |  |    || ||   |||| | | |   |   || | | | || | |        
TGCTGGGAGACACACCCCCGGAACTTTCACCAATCAGCTGCAGAACACCTTCAGCGAGCCCAGGCTTCTAATTTTGACGGATCCTCGAACTGATCACCAGgttagggtt--ttttggatggcattgctgggcgcggat---catagtgtagagggca-atagttggggtgcattgagcggaggtgggcagtggtgttcttcgtttt

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ20712 (Selaginella moellendorffii), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgtgatggtgaactaatagttatgcgtaattgtttgcag
||| ||||||||||||||||| || ||||||||||||||||||| |||||||   ||||||||||| ||||| || || || ||  | || || |||||||| |        |||| |     | |||      |  | |    | | ||| | |    || || |      ||    ||| | |              
CGCCGGGAGGCACACCCCTGGAACTTTCACCAATCAGCTCCAGAACTCCTTCTCGGAGCCTCGCCTCCTCATTCTCACGGATCCGCGCACCGATCACCAGgtaag-------tttgctcgctcgatctagcacgacttttttcctcattggtttccttcttttgttttgtgtctgtgtgttcttccag-----------

upper sequence: GRMZM2G145308_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ32709 (Selaginella moellendorffii), 5'ss of exon 2

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaagttttgtttcaaagttcttaagaaatatatgcattgagtgggtgctgcattgtgatg-gtgaactaatagttatgcgtaattgtttgcag
||| ||||||||||||||||| || ||||||||||||||||||| |||||||   ||||||||||| ||||| || || || ||  | || || |||||||| |        |||| |     | |||      |  | |    | | ||| | |    || || |  |||      |  |  | |              
CGCCGGGAGGCACACCCCTGGAACTTTCACCAATCAGCTCCAGAACTCCTTCTCGGAGCCTCGCCTCCTCATTCTCACGGATCCGCGCACAGATCACCAGgtaag-------tttgctcgctcgatctagcacgacttttttcctcattggtttccttcttttgttttgtgtgtgtgtgttcttccag------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|31350320|gb|CD434677.1|CD434677

EST:     TCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAG                         CCAATCAAGGAGTCTGCTCTGGGGAACATCCCGACCATTGCTTTCTGCGAC
genomic: TCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaa ... ttgtttgcagCCAATCAAGGAGTCTGCTCTGGGGAACATCCCGACCATTGCTTTCTGCGAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 8 (upstream exon), 47 (downstream exon)
Score: 57

CGCTGGGAGGCACACCCCTGGTACCTTCACCAATCAGCTCCAGACCTCCTTCAGTGAGCCTCGCCTGCTCATCCTTACTGACCCAAGGACTGACCACCAGgtgaagagaa ... ttgtttgcagCCAATCAAGGAGTCTGCTCTGGGGAACATCCCGACCATTGCTTTCTGCGACACTGACTCTCCCATGCGGTATGTTGATATCGGCATCCCAGCCAACAACA

Block sizes: 8 (upstream exon), 47 (downstream exon)
Score: 38

Block sizes: 8 (upstream exon), 47 (downstream exon)
Score: 31