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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgttatccagtctttctttctatatgcagaagcactgactaatatatgttaggatcgttagttatgcacattttgcatcctattacttgttctgcagccttttccacttcaacggtaaattaaatcacttatatcaatgtttccag

Basic information

species Zea mays
transcript GRMZM2G122064_T01
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G122064_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os08g43380.1 (Oryza sativa), 5'ss of exon 2
GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgttatccagtctttctttctatatgcagaagcactgactaatatatgt-taggatcgttagttatgcacattttgcatcctattacttgttctgcagccttttccacttcaacggtaaattaaatcacttatatcaatgtttccag
 |||||| | ||| ||||||  || ||||| | |||||||||  || ||||||||||||||||||| ||||| |||||||||||||| || ||| |||| ||||||  | |    | || |    |||||    |    || |||| | ||  | |  | | ||||| ||| || ||||   |   ||||  | ||||   |       | |  |  | | ||||||||  ||      ||| |||||||||
AAAACGGCCACTTTCTTGTCGGCATTGGAACCATCTATTTTCGTCAGATGGGAAGCTTCGTGATGGAGGAAGGAAGTTTCTAAAGAAAGTCCGCGGTGGAgtaagtcctgtt-gttattccaggttatatttgtactggctaactagtgtagatgtgcggttgttagctatacaaatttctgaa--tattcatagttccata-------caatgttgaagctaaattaatcca------tcattgtttccag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71446024|gb|DR827074.1|DR827074
EST:     GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGG                         GGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
genomic: GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
EST: gi|148954876|gb|EC900353.2|EC900353
EST:     GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGG                         GGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
genomic: GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
EST: gi|149112489|gb|EE295360.2|EE295360
EST:     GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGG                         GGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
genomic: GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
EST: gi|148967009|gb|EE018161.2|EE018161
EST:     GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGG                         GGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
genomic: GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
EST: gi|148965580|gb|EE016411.2|EE016411
EST:     GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGG                         GGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT
genomic: GGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 179

GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 108

GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 115

GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 189

GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgtta ... atgtttccagGGGATTGAACCGGGCATAAGGGCTGAAGTTTGGCCATTTCTGCTTGGAGT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GAAACGGGCGCTTACTTGTCAACACTGGAATCGTCTATTTTCAGCAAATGGGAAGCTTCGTGATGGGGGAAGAAAGTTTCTAAAGAAGGTTCGCAGTGGGgtaagtgttatccagtctttctttctatatgcagaagcactgactaatatatgttaggatcgttagttatgcacattttgcatcctattacttgttctgcagccttttccacttcaacggtaaattaaatcacttatatcaatgtttccag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG