ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

GATGGACTATGCGTTTGAGTTCATCATCAACAACGGCGGAATCGACACCGAGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcgcttcatggtcttccttgatcttccaaatatcagtgtcatttttttcttcttgctgattttatgtgttgggttcatcgcag

Basic information

species	Zea mays
transcript	GRMZM2G073465_T03
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G073465_T03 (Zea mays), 5'ss of exon 2
lower sequence: Vv18s0001g09990.t01 (Vitis vinifera), 5'ss of exon 2

GATGGACTATGCGTTTGAGTTCATCATCAACAACGGCGGAATCGACACCGAGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcgcttcatggtcttccttgatcttccaaatatcagtgtcatttttttcttcttgctgattttatgtgttgggttcatcgcag
 ||||||||||| ||||||||||| ||||||||||| || || ||| | || || |||||||| ||||| |   | || ||  | |||||     | |||||   | |   ||| ||    |   |    | ||||| |   |   ||        || || |||||| | |    |   ||  | ||||
TATGGACTATGCCTTTGAGTTCATTATCAACAACGGAGGCATTGACTCTGAAGAAGATTACCCCTACAAAGCTTCCGATGGCAGATGTGACCAGTATAGGgtcggtattcattcttgaaaatttatacaataccaaagacaggaaacaggaagaaattttttctgattcttta--gtaatttttttgcag

upper sequence: GRMZM2G073465_T03 (Zea mays), 5'ss of exon 2
lower sequence: PP1S49_32V6.1 (Physcomitrella patens), 5'ss of exon 2

GATGGACTATGCGTTTGAGTTCATCATCAACAACGGCGGAATCGACACCGAGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcgcttcatggtcttccttgatcttccaaatatcagtgtcatttttt------tcttcttgctgatt---ttat-gtgttgggttcatcgcag----------------------------------------------------------------------------------------------
 ||||| ||||||||||| ||||||||||| || || |||||||| ||||||||||| ||||| |||||||  || || || |  |||||||    |||||||   |    |   | |  || ||  || |    |||  |   || || ||||      | |    | |||||   ||||  |||  |||    |                                                                                                  
TATGGATTATGCGTTTGATTTCATCATCAAAAATGGTGGAATCGATACCGAGGAGGACTACCCCTACAAGGCAACTGATGGCCAATGTGATGAGGCCAGGgtaaactctaatgttttgattttctccattt---gaattactacgttatgttttacaaaatttcaacggtgattagtttatcatgtgaggtatggcattttgttcgcagatttagccagaatttggcattcatttgcatcttggtgttgttcgatttctctcagaatcttattagtgacgattctctcttgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211107453|gb|FL045700.1|FL045700

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

EST: gi|21843478|gb|BQ704059.1|BQ704059

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTGCTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

EST: gi|149077229|gb|EE165898.2|EE165898

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

EST: gi|89762501|gb|DY690053.1|DY690053

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

EST: gi|211107449|gb|FL045696.1|FL045696

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

EST: gi|211107451|gb|FL045698.1|FL045698

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

EST: gi|149078310|gb|EE167076.2|EE167076

EST:     AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGG                         AAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC
genomic: AGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 24 (downstream exon)
Score: 296

GATGGACTATGCGTTTGAGTTCATCATCAACAACGGCGGAATCGACACCGAGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcg ... ttcatcgcagAAAAACGCCAAGGTTGTTACTATCGACAGCTATGAAGATGTACCAGCGAACAGTGAGAAAAGTCTGCAGAAGGCAGTTGCGAACCAGCCCATCAGTGTTG

Block sizes: 49 (upstream exon), 24 (downstream exon)
Score: 1749

Block sizes: 49 (upstream exon), 24 (downstream exon)
Score: 1577

Block sizes: 49 (upstream exon), 24 (downstream exon)
Score: 973

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GATGGACTATGCGTTTGAGTTCATCATCAACAACGGCGGAATCGACACCGAGGAGGATTACCCTTACAAGGGCACGGACGGACGGTGTGATGTCAACAGGgtatttttcgcttcatggtcttccttgatcttccaaatatcagtgtcatttttttcttcttgctgattttatgtgttgggttcatcgcag

- - - - - - - - - - - - - - - - - - - - - - - - -  GGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAT