ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CAAAGCAAGGCTTTTCCTGGTCCTTCCTGATGCATTTTTGGATGCTTTTCTCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaatttagtgcagtttaacaagtagaagcatttctaattgttctctctttatgtaaattcatacaatctaaactttgtagacttccacatgtaa...

Basic information

species	Zea mays
transcript	GRMZM2G064366_T01
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G064366_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os11g34360.1 (Oryza sativa), 5'ss of exon 2

CAAAGCAAGGCTTTTCCTGGTCCTTCCTGATGCATTTTTGGATGCTTTTCTCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagcta-atttagtgcagtttaacaagtagaagcatttctaat--tgttctctctttatgtaaattcatacaatctaaactttgtagacttccacatgtaa
 ||||||||||||||||| || ||||||||||| |||||||||||||| |||||||| ||||||||||||||||| ||||||||||| |||||| ||||||||||||| ||||  || | |||  |  ||    || ||| ||||  || |  |||  |  ||      ||| ||      ||      || | | | | |  
GAAAGCAAGGCTTTTCCTAGTTCTTCCTGATGCCTTTTTGGATGCTTTCCTCATACTTTGGATTTTCACTTCTCTCTCCCGCACACTTGAGAAGTTACAGgtaagctatatttgatgtaatttttcttgtct--gcttttataatgatgattactccctccgtcccaaaatataacaacttctacaccaacattctcttctc-

upper sequence: GRMZM2G064366_T01 (Zea mays), 5'ss of exon 2
lower sequence: Vv12s0034g01090.t01 (Vitis vinifera), 5'ss of exon 2

CAAAGCAAGGCTTTTCCTGGTCCTTCCTGATGCATTTTTGGATGCTTTTCTCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaatttagtgcagtttaacaagtagaagcatttctaattgttctctctttatgtaaattca-tacaatctaaactttgtagacttccacatgtaa-----------------------------------------------------------------------
 | ||||||||| ||| | |||||||||||||||||  ||||||| ||| | ||| | |||||||||||||| |||||    ||| ||||| | |||||||||    | ||    |     || ||  |   ||      |  ||  | ||  || |  ||    ||  |  |||  | ||||     | |    ||                                                                           
AAGAGCAAGGCTCTTCTTAGTCCTTCCTGATGCATTCCTGGATGCATTTTTGATATTGTGGATTTTCACTTCCCTTTCAAAGACATTAGAGCAACTACAGgta--tcagttcttcgtctactatcagttttgagttccagttgttaatatcagttaaaatagtggcaacaacatcagagcttttctttcctttttatcctttccttttcttttttactatttttatttttcattcattatcatattctgatgctataattatttgtgttcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|67024546|gb|CO453295.1|CO453295

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|148967311|gb|EE018517.2|EE018517

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|76289905|gb|DV029473.1|DV029473

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|22491183|gb|BU051106.1|BU051106

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|83278764|gb|DV942772.1|DV942772

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|149112569|gb|EE295431.2|EE295431

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|211376336|gb|FL031209.1|FL031209

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|19272383|gb|BM888639.1|BM888639

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|19272497|gb|BM888753.1|BM888753

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|148959137|gb|EE010606.2|EE010606

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|211464044|gb|FL176493.1|FL176493

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCAMAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|148960354|gb|EE153613.2|EE153613

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

EST: gi|148971674|gb|EE022398.2|EE022398

EST:     TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAG                         GCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA
genomic: TCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 127

CAAAGCAAGGCTTTTCCTGGTCCTTCCTGATGCATTTTTGGATGCTTTTCTCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaat ... ctttcaccagGCAAGGAGGAGTTCTGTGAAATTGGATATATACAGAAAGTTCACAAATGCATTGGCTGTTTCTGTGATAGCTTCAGTTGCCTGGATTGGTTATGAG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 180

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 216

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 340

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CAAAGCAAGGCTTTTCCTGGTCCTTCCTGATGCATTTTTGGATGCTTTTCTCATACTCTGGATTTTCACTTCTCTTTCCCGCACACTAGAGAAGCTACAGgtaagctaatttagtgcagtttaacaagtagaagcatttctaattgttctctctttatgtaaattcatacaatctaaactttgtagacttccacatgtaa

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -aagtagaa