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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcattctaaactgtacaatttttttttcttcttcttctagcttgctaggctggactatctctggtttttaattcgaaaaataggaacagaatct...

Basic information

species Zea mays
transcript GRMZM2G060800_T02
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G060800_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os11g08300.2 (Oryza sativa), 5'ss of exon 2
---GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcattctaaactgtacaatttttttttcttcttcttctagcttgctaggctggactatctctggtttttaattcgaaaaataggaacagaatct---------
   |||||||| ||| | ||||||||||| ||  | || ||||| ||| | ||||| || || ||||| ||  |||||||||| ||||||||||||||| |||||||      |||  || ||   ||  | | |  | ||  ||  |   ||  |     |||  |      | |        | |||| |   | |           
CAGGTACCTGCCGCCCTCACTACATTCCCATCAACTGCACAAATTGTGTCAGAGCCTCTTGGTGTCGTCCTAGTCATCTCAGCATGGAACTACCCTTTCTgtaagtt------aaatggtgca---ttcatgtttcatactcgtaattgcataaacaccgttattccatagagtaatgctattgagtagggattaattgcaatttttgg

upper sequence: GRMZM2G060800_T02 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os11g08300.1 (Oryza sativa), 5'ss of exon 3
GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcattctaaactgtacaatttttttttcttcttcttctagcttgctaggctggactatctctggtttttaattcgaaaaataggaacagaatct---------
|||||||| ||| | ||||||||||| ||  | || ||||| ||| | ||||| || || ||||| ||  |||||||||| ||||||||||||||| |||||||      |||  || ||   ||  | | |  | ||  ||  |   ||  |     |||  |      | |        | |||| |   | |           
GTACCTGCCGCCCTCACTACATTCCCATCAACTGCACAAATTGTGTCAGAGCCTCTTGGTGTCGTCCTAGTCATCTCAGCATGGAACTACCCTTTCTgtaagtt------aaatggtgca---ttcatgtttcatactcgtaattgcataaacaccgttattccatagagtaatgctattgagtagggattaattgcaatttttgg

upper sequence: GRMZM2G060800_T02 (Zea mays), 5'ss of exon 2
lower sequence: EFJ20694 (Selaginella moellendorffii), 5'ss of exon 3
GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttc-----attctaaactgtacaatttttttttcttcttcttctagcttgctaggctggactatctctggtttttaattcgaaaaataggaacagaatct
||  |  |  | || |  |  || || || ||||   ||||||| || |||||||||||||| | |||| | ||||| |||||||||| ||||||| || |||       ||||||      |  | |  | |||  | ||||| |  | || |||                                              
GTCTCGACATCGATGATCAGCTTTCCTTCATCCGGAGAAATCGTCCCAGAGCCCCTCGGCGTTGCGCTCGTTATCTCTGCCTGGAACTTCCCTTTCCgtgagtctctggaattctagttctcatgactagtgttttcttttctttt--cctg-tag----------------------------------------------

upper sequence: GRMZM2G060800_T02 (Zea mays), 5'ss of exon 2
lower sequence: EFJ32729 (Selaginella moellendorffii), 5'ss of exon 3
GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcattctaaactgta-caatttttttttcttcttcttctagcttgctaggctggactatctctggtttttaattcgaaaaataggaacagaatct
||  || |  | || |  |  || || || ||||   ||||||| || |||||||||||||| | |||| | ||||| |||||||||| ||||||  || |||   |   || ||||  | | || |  |   |  | || |   ||  |   ||| |                                        
GTCTCTACATCGATGATCAGCTTTCCTTCATCCGGAGAAATCGTCCCAGAGCCCCTCGGCGTTGCGCTCGTTATCTCTGCCTGGAACTTCCCTTTTCgtgagtctctggaaatctgtggctagttctcatgactagtgttttcttttcttttcctgtag---------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78123147|gb|DV541531.1|DV541531
EST:     GGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA                         TACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
genomic: GGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
EST: gi|58029607|gb|CX725034.1|CX725034
EST:     GGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA                         TACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
genomic: GGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
EST: gi|6564146|gb|AW231768.1|AW231768
EST:     GTGCTCATCATCTCAGCCTGGAACTACCCTTTCA                         TACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
genomic: GTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
EST: gi|78111538|gb|DV529935.1|DV529935
EST:     GGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCA                         TACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG
genomic: GGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 74

GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTGTTCTGAAGCCATCAGAAATAGCGCCAGCTACCTCGTCACTGTTAGCAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 565

GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTGTTCTGAAGCCATCAGAAATAGCGCCAGCTACCTCGTCACTGTTAGCAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 477

GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTGTTCTGAAGCCATCAGAAATAGCGCCAGCTACCTCGTCACTGTTAGCAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 422

GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcat ... tccactgtagTACTCTCTATCGACCCAGTCATTGGAGCAATCGCTGCTGGAAATGCTGTTGTTCTGAAGCCATCAGAAATAGCGCCAGCTACCTCGTCACTGTTAGCAAA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTACCTGCGGCCATAACTACATTCCCGTCCTCCGCCCAAATCGTGCCGGAGCCCCTCGGCGTCGTGCTCATCATCTCAGCCTGGAACTACCCTTTCAgtaagttcattctaaactgtacaatttttttttcttcttcttctagcttgctaggctggactatctctggtttttaattcgaaaaataggaacagaatct

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - gaaaaata
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gaacagaa