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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta...

Basic information

species Zea mays
transcript GRMZM2G050925_T01
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os02g34460.1 (Oryza sativa), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggg-gacgtcttcaaactgtttccttatta-
|||||||||||||||||||| |||||||||||||||||||||||| | |||  ||      |  ||  ||  | | |        | | || |     | | |||  |        |   ||| ||    || || |  |    | |||  
ATCATCCCCAAGAAGAACCGGAATGAGATCTGCAAGTACCTCTTCCAAGgttggctcactctgctctccttgattggtttcgatctca-ttacaa-aatggattgcgtaaacaggaaattgggtgattgttttaatccctcgtttaattgg

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os04g35090.1 (Oryza sativa), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgc-taaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta
|||||||| |||||||||||||||||||||||||||||||||||| | |||  | | || || ||||   |   | |   |||     ||| | |  |    |  |   |  | | |||  | |   ||  |   |  |||| |  |   
ATCATCCCGAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCCAAGgtcagtggttcttggtccctatttgcacctcgtaatcgtactagaggcgtagagccgccgccatgtttagtggagattgttgttttag-gtttgcaaaacg

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA11G02410.3 (Glycine max), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcga----------ttattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtctt-caaactgtttccttatta
||||| ||| |||||||||| || ||||||||||| ||||||||| | |||  |  |          || |     |       |  |||| | || |     ||  | ||  ||| |    |||   | | |   |  || | ||              
ATCATTCCCGAGAAGAACCGTAAAGAGATCTGCAAATACCTCTTCCAAGgtccgttagttaacattgttgtctcctcttgtgctttacagttgtgttaaaattgtaaggtgtgtgtgtttgattcagagggagtttgctttgcgaag-------------

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA05G37470.1 (Glycine max), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta---
||||||||  ||||||||||||| ||||||||||| ||||||||| | |||| | |  |   | |     |    || |  |   |   | |    ||||    || ||| |    |           |||      || |   |  |    
ATCATCCCGGAGAAGAACCGCAAGGAGATCTGCAAATACCTCTTCCAAGgtaggtgccttagcatttatca----cgaatcatcttcgttcgttagggtta--cgtctcgttgaacaatgaatcttacctttcgttggtgtttatggtgcag

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA08G02070.1 (Glycine max), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcga-ttattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta
||||| ||  ||||||||||||| ||||||||||| ||||||||| | |||| | |  |||    | |  |    |  |     | | |     |    ||   |  ||   | |  | |  ||  |                       
ATCATTCCTGAGAAGAACCGCAAGGAGATCTGCAAATACCTCTTCCAAGgtaggtgccttagaatttcattggggttacgtctcgttgaagaatgaatcttaccttcttttttcttaatgtcggtgcag---------------------

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA11G02410.2 (Glycine max), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta
||||| ||| |||||||||| || ||||||||||| ||||||||| | |||           |||  | |||  | |  ||    |  |||| | |     ||||| |     |      | |   || ||  |               
ATCATTCCCGAGAAGAACCGTAAAGAGATCTGCAAATACCTCTTCCAAGgt----------ccgttagttaacattgttgtctcct--cttgtgctttacagttgtgttaaaattgtaaggtgtgtgtgtttgattcag----------

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA01G43110.1 (Glycine max), 5'ss of exon 2
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta
||||| ||| |||||||||| || ||||||||||| ||||||||| | |||  |  | |   | |   ||   || |   |        ||| |||  |   ||||  ||  |  | |     |                         
ATCATTCCCGAGAAGAACCGTAAAGAGATCTGCAAATACCTCTTCCAAGgtccgttagttaacattgtctcctcttgtgctttacgg--ttgtgtt--taaattgtaacgtgtgtttgattcag-------------------------

upper sequence: GRMZM2G050925_T01 (Zea mays), 5'ss of exon 2
lower sequence: EDP00807 (Chlamydomonas reinhardtii), 5'ss of exon 1
---------------------------------------------------ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgtt---ggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta------------------------------------------------------------------------
                                                    | |||||||||||||||||||  ||| | | |||||||||||||||||||  ||     || ||  || |     |   | | ||   ||| |     ||    | |   |     ||||    |   |||  |  || |  | |                                                                              
GGTCCCAAACCCCCACTTGGTGGCTGTCTCTTCCTCAGGCCAGCAAAGATGCTTATCCCCAAGAAGAACCGCAGGGAGGTGTACAAGTACCTCTTCAAGGgtgagcatcagttggtatgcgacctcggtgttcgcgttctttgaggcccggggacatggacgccgggcctagcgacgcttgtcggcgcacagcctgcggcgcgcgctttcttcggccgcaaatttatttccccaatgtatgcttacacacctgtatttgctcgttgtgcgcgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|28576004|gb|CB281896.1|CB281896
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|76020050|gb|DT947220.1|DT947220
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|211448197|gb|FL410300.1|FL410300
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|18170762|gb|BM340602.1|BM340602
EST:     GCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: GCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|32807051|gb|CD959285.1|CD959285
EST:     GATCTACAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: GATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|32807428|gb|CD959662.1|CD959662
EST:     GATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATACCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: GATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|32824632|gb|CD964354.1|CD964354
EST:     GATCTACAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: GATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|20301451|gb|BQ164394.1|BQ164394
EST:     GATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACATG
genomic: GATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACA-G
EST: gi|22542952|gb|BU093390.1|BU093390
EST:     CCCCAAGAAGAACCGCAA-GAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGCATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: CCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|78074399|gb|DV502833.1|DV502833
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|78082396|gb|DV510808.1|DV510808
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|94475894|gb|EB704852.1|EB704852
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGC---TACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|22935805|gb|BU572080.1|BU572080
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|94475893|gb|EB704851.1|EB704851
EST:     AGAAGAACCGCAATGAGATCTGC---TACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: AGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|50328582|gb|CO523708.1|CO523708
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|31358616|gb|CD442973.1|CD442973
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|33101552|gb|CF061512.1|CF061512
EST:     ACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|148995686|gb|EE034906.2|EE034906
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|32807844|gb|CD960078.1|CD960078
EST:     GATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: GATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|120480896|gb|EH211080.1|EH211080
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|211321903|gb|FL420982.1|FL420982
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|32917369|gb|CF022181.1|CF022181
EST:     ATCATCCCCAAGAAAAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|22935565|gb|BU571840.1|BU571840
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCG-GCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|67017839|gb|CO446588.1|CO446588
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|60345271|gb|DN212244.1|DN212244
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
EST: gi|211366018|gb|FL393816.1|FL393816
EST:     ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGG                         AGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA
genomic: ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 237

ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGATTGATGTGCCCAACCTCCAGGTGATCAAGCTCATGCAGAGCTTCAAGTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 233

ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGATTGATGTGCCCAACCTCCAGGTGATCAAGCTCATGCAGAGCTTCAAGTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 343

ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGATTGATGTGCCCAACCTCCAGGTGATCAAGCTCATGCAGAGCTTCAAGTC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 111

ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgatt ... gctgctgcagAGGGCGTGCTGTATGCCAAGAAGGACTACAACCTGGCGAAGCACCCACAGATTGATGTGCCCAACCTCCAGGTGATCAAGCTCATGCAGAGCTTCAAGTC


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ATCATCCCCAAGAAGAACCGCAATGAGATCTGCAAGTACCTCTTCAAGGgtatgcgattattcgtccgctaaactcgcagtagggtaacttgcgttggttggttgtttcgctttctaggaggggacgtcttcaaactgtttccttatta

- - - -  AAGAAG
- - - - - - AAGAAC
- - - - - - - - - - - - GAGATC