ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

CGTTTTTATGGGCTCCTTAGAAGTTCCAGCCCAGGGGTCTGAAACTATTAAAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttccatgtcacccgttgttattgtggcatcttccataatgatttatttcttaactcatctgcttctgtgcatgaaactatgtgtag

Basic information

species	Zea mays
transcript	GRMZM2G043983_T03
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G043983_T04 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os03g20750.1 (Oryza sativa), 5'ss of exon 3

CGTTTTTATGGGCTCCTTAGAAGTTCCAGCCCAGGGGTCTGAAACTATTAAAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttccatgtcacccgttgttattgtggcatcttccataatgatttatttcttaactcatctgcttctgtgcatgaaactatgtgtag
 ||||||||||| ||||||||||||||| | |||    |||||||||| |||||||||||  | ||||| ||||| |||||||||||| ||||||||||||||||| |  |||  |     | ||||       | || |||| ||   |||||  |||||  |  | || ||   ||| || | | |  ||
TGTTTTTATGGGTTCCTTAGAAGTTCCATCTCAGTCAACTGAAACTATAAAAGTTCCAACATCACATTACGAATTCGGTGCCAATTTTATAGATCCAAAGgtttgtctgtatggaattgaatattatga--attttttgcatagtg---tattttgtaacttgt--gattttgaa-atggaattgtctacag

upper sequence: GRMZM2G043983_T04 (Zea mays), 5'ss of exon 2
lower sequence: Vv03s0097g00590.t01 (Vitis vinifera), 5'ss of exon 3

CGTTTTTATGGGCTCCTTAGAAGTTCCAGCCCAGGGGTCTGAAACTATTAAAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttccatgtcacccgttgttattgtggcatcttccataatgatt---tatttcttaactcatctgcttctgtgcatg---aaactatgtgtag-----------------------
||| || |||||  ||   ||| ||||  | ||    ||||||||||||||| | ||||||||||||||||| ||||| || || ||| |||| ||||||||  |  |   | |      ||  | || ||| |  |  | || || |    |  |    ||||  | || ||   || |||   ||   | |  | |                       
CGTGTTCATGGGGCCCACTGAAATTCCTTCTCAATCCTCTGAAACTATTAAAATCCCAACTGCTCATTATGAGTTTGGCGCTAACTTTATAGACCCAAAGgtaggcctaatttttcagatttacttttatgggaaatatcttattggtagtgtgatgtgcaactagtttgttttcatgaatgttgaatgaaagcattggagcatttttggttgcttaacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|148982521|gb|EE028361.2|EE028361

EST:     AAGTTCCAACTGCTCATTATGAATTTGGTGCCANTTTTTTAGATCCAAAG                         TTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTG
genomic: AAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttcc ... ctatgtgtagTTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTG

EST: gi|6101993|gb|AW126463.1|AW126463

EST:     GAATTTGGTGCCAATTTTTTAGATCCAAAG                         TTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTG
genomic: GAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttcc ... ctatgtgtagTTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTG

EST: gi|78089042|gb|DV517435.1|DV517435

EST:     AAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAG                         TTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTG
genomic: AAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttcc ... ctatgtgtagTTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 274

CGTTTTTATGGGCTCCTTAGAAGTTCCAGCCCAGGGGTCTGAAACTATTAAAGTTCCAACTGCTCATTATGAATTTGGTGCCAATTTTTTAGATCCAAAGgtttgtttcc ... ctatgtgtagTTAATGCTCATTGGAAGGGTGATGACAGATGGAAGGCTTAATGCTCGCGTGAAATGTGATTTGACAGACAATCTGATGCTGAAAGTAAATGCACAG

Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 49

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 77