ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

TAGTTCGACCACTTCCTCATGGAGTTAAACTTCATGCACTCATTGATGCTTGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttatattatgcatggtcatttgatttgtctagtagggcctctgaggcttcccctcccctgtgtgctggagaggaggcttgagggtttggtcttgg...

Basic information

species	Zea mays
transcript	GRMZM2G035928_T01
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G035928_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA08G45470.1 (Glycine max), 5'ss of exon 2

TAGTTCGACCACTTCCTCATGGAGTTAAACTTCATGCACTCATTGATGCTTGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttatattatgcatggtcatttga---tttgtctagtagggcctctgaggcttcccctcccctgtgtgctggagag-gaggcttgagggtttggtcttgg
| ||| ||||  |||||||||||| ||| || |||||||| |||||||| || |||||||| ||||  || |||||||| | ||| |||||||||||||||| |  | | | | | | |    ||  |   | ||  | ||    | ||      ||   |||   ||  |   | |  | || |    | |      |||   
TTGTTAGACCTATTCCTCATGGAGCTAAGCTACATGCACTAATTGATGCCTGCCATAGTGGCACTGTTCTAGATTTGCCATTTCTTTGCAGAATGAACAGgttacct-tctaaagaaagtctgttatagtttctgcatggtttaacttcctgaattttggctctgttgactttagcaatgtgatggccaatgaaaaattct---

upper sequence: GRMZM2G035928_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA01G10880.1 (Glycine max), 5'ss of exon 2

TAGTTCGACCACTTCCTCATGGAGTTAAACTTCATGCACTCATTGATGCTTGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttat--attatgcatggtcatttgatttgtctagtagggcctctgaggcttcccctcccctgtgtgctggag--aggaggcttgagggtttggtcttgg
| ||  ||||  |||||||||||| ||| || |||||||| |||||||| || |||||||| ||||  || |||||||| | ||| |||||||||||||||| |  |    |  ||     || ||    | ||  | ||    | |    |  ||   |||   ||  |  | |      || | |  | |      ||    
TTGTCAGACCTATTCCTCATGGAGCTAAGCTACATGCACTAATTGATGCCTGCCATAGTGGCACTGTTCTAGATTTGCCATTTCTTTGCAGAATGAACAGgttaccttctaaagatagtctgttatagtttctgcatggtttaactttatggattttggctctgttgacttttagcattgtgatggtcaatgaaaaattc----

upper sequence: GRMZM2G035928_T01 (Zea mays), 5'ss of exon 2
lower sequence: Vv16s0013g00220.t01 (Vitis vinifera), 5'ss of exon 2

TAGTTCGACCACTTCCTCATGGAGTTAAACTTCATGCACTCATTGATGCTTGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttatattatgcatggtcatttgatttgtcta-gtagggcctctgaggcttcccctcccc-tgtgtgctggagaggaggcttgagggtttggtcttgg--------------------------------------------------------------------------------------------
| ||| | || ||||||||||||||||| ||||||||| |||||||||| || |||||||| ||||   | ||||| || |  ||||| ||||||||||||| | || | ||  | ||   ||    |    |||  ||     |  || |              | |     | |  | |          ||  ||||                                                                                               
TTGTTAGGCCTCTTCCTCATGGAGTTAAGCTTCATGCAATCATTGATGCATGCCATAGTGGCACTGTGTTAGATTTACCATTCCTCTGTAGAATGAACAGgtcagttgttttg-gtatttccaaacaacacttctttgtgaataccatgtgataagtggagagagtataaaaagaaatgaaaaaccataaattctgtctatatatgctaactttttaataggattcaattccaattttcccttgtccatttttttagtccaccctgcttatagatgtttcctttgtggccatag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|91875164|gb|EB405121.1|EB405121

EST:     GCAGAATGAACAG                         GAGGGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: GCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|148976593|gb|EE025939.2|EE025939

EST:     GATTTGCCTTATCTCTGCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: GATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|50337462|gb|CO532588.1|CO532588

EST:     GCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: GCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|91055205|gb|EB165623.1|EB165623

EST:     AGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: AGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|78079402|gb|DV507814.1|DV507814

EST:     TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|76911371|gb|DV164249.1|DV164249

EST:     TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|193502060|gb|AM937871.1|AM937871

EST:     TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|89246802|gb|DY618589.1|DY618589

EST:     TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

EST: gi|93016582|gb|EB642102.1|EB642102

EST:     TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAG                         GAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA
genomic: TGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 68

TAGTTCGACCACTTCCTCATGGAGTTAAACTTCATGCACTCATTGATGCTTGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttata ... gatattacagGAGTGGGCAATATGTATGGGAAGATCACCGACCGCGATCTGGTGTCTGGAAAGGCACTAGTGGTGGGGAAGCTATTTCCTTTAGTGGTTGTGATGATGAT

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 147

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 136

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 130

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TAGTTCGACCACTTCCTCATGGAGTTAAACTTCATGCACTCATTGATGCTTGTCATAGTGGGACTGCACTTGATTTGCCTTATCTCTGCAGAATGAACAGgtaatttatattatgcatggtcatttgatttgtctagtagggcctctgaggcttcccctcccctgtgtgctggagaggaggcttgagggtttggtcttgg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ccctccc