ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TGAGAAGGCATACATTATGAGAGATATGAAGAAGGAAAAGGAGCTAGGTTTTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctctaatttcttatctatgaagagtccaattgaaatgagaaaaaagaatagtgatagaggacagttgtattctcttttatgtgaagagaaaactcaacatttacattatgcttacgttttctagtatggtcataatgttacctgaggtttccaaaaattcgtttcag

Basic information

species	Zea mays
transcript	GRMZM2G028685_T01
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G028685_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os01g71256.1 (Oryza sativa), 5'ss of exon 3

TGAGAAGGCATACATTATGAGAGATATGAAGAAGGAAAAGGAGCTAGGTTTTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctctaatttcttatctatgaagagtccaattgaaatgagaaaaaagaatagtgatagaggacagttgtattctcttttatgtgaagagaaaactcaacatttacattatgcttacgttttctagtatggtcataatgttacctgaggtttccaaaaattcgtttcag
||||||   ||||||| |||| ||||| |||||||||||||||||||| |||||||||||||||||||| ||| ||||  |||||||||| ||| |  ||||  |  | || ||| ||| || |||| | |  |||  ||||||||| ||| | |||  ||    | || || |||| ||||  |||  |||||| |  | ||| |   || |||||  |  |       |    |  | ||||||||| |   ||     ||||   | |||
TGAGAAAAGATACATTCTGAGGGATATAAAGAAGGAAAAGGAGCTAGGCTTTAGCATGCCTGTCTCACTAAGCCGAGCTTGCAAAAAACCTTTGTTAGAGgtgatattttagtttattagct-tgaaaaatataatcaaaatgagaacaaatactagcaatttttggcaattatattatcttg-atgccaagagatactttaactt---cagtatgccaaaat------atgaaatgtttatgttacctaaaaatttattgaatt---tccag

upper sequence: GRMZM2G028685_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA15G10570.1 (Glycine max), 5'ss of exon 6

TGAGAAGGCATACATTATGAGAGATATGAAGAAGGAAAAGGAGCTAGGTTTTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctctaatttcttatctatgaagagtcca-attgaaatgagaaaaaagaatagtgatagaggacagttgtattctcttttatgtgaagagaaaactcaacatttacattatgcttacgttttctagtatggtcataatgttacctgaggtttccaaaaattcgtttcag
|||||     |||||  |||| |||  ||| |||||||||||| ||||||| |||||||| ||||||||    ||  |  ||||   | |||           |   |  ||  |  ||| | || |   | || | ||||| ||| |  ||  ||  | ||   ||     || ||  | ||| |   |  |  |      || | | |  || |                                                          
TGAGAGAAATTACATATTGAGGGATGCGAAAAAGGAAAAGGAGTTAGGTTTCAGCATGCCAGTCTCACT----AGCACGTGCAATCCAGCATC---------CTCTTTTAAAGgtaatatatgtggaacatacatagtgaaaggagtagtaa--atcataatcttgggataatggatg-tgtttcaccagttggaattgtaaaaaagtcattttgt----------------------------------------------------------

upper sequence: GRMZM2G028685_T01 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA03G36660.1 (Glycine max), 5'ss of exon 6

TGAGAAGGCATACATTATGAGAGATATGAAGAAGGAAAAGGAGCTAGGTTTTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctctaatttcttatctatgaagagtccaa-ttgaaatgagaaaaa-agaatagtgatagaggacagttgtattctcttttatgtgaagagaaaactcaacatttacattatgcttacgttttctagtatggtcataatgttacctgaggtttccaaaaattcgtttcag
||||||    |||||| |||| |||||||| |||||||||||  |||| |||| ||||||||| || |||    ||||  |  | || ||  | ||  |||| ||| | ||    ||| | |  |    | |||| ||   |||     |  | | | |   || |     ||||  | |   || |                                                                                        
TGAGAAAAATTACATTCTGAGGGATATGAAAAAGGAAAAGGAAATAGGCTTTAACATGCCTGTTTCTCTTGCTCGAGCAAGACAGAAGCCGCTCCTAAAGgtattcttgtataaacttttgttagttatgcccaaattcttatgcttccatcatatttgccttaaaatctggttgcttgcctcttcag---------------------------------------------------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|71311783|gb|DR792346.1|DR792346

EST:     TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAG                         GGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG
genomic: TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctct ... ttcgtttcagGGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG

EST: gi|60339789|gb|DN206762.1|DN206762

EST:     AAAAACCATTGCTTCAG                         GGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTGGCAAGGAGCTTCTG
genomic: AAAAACCATTGCTTCAGgttttcctct ... ttcgtttcagGGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG

EST: gi|60343103|gb|DN210076.1|DN210076

EST:     TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAG                         GGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG
genomic: TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctct ... ttcgtttcagGGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG

EST: gi|60350264|gb|DN217237.1|DN217237

EST:     TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAG                         GGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG
genomic: TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctct ... ttcgtttcagGGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG

EST: gi|101383529|gb|EB813820.1|EB813820

EST:     TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAG                         GGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG
genomic: TTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctct ... ttcgtttcagGGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 3 RNA-Seq libraries.

Block sizes: 33 (upstream exon), 44 (downstream exon)
Score: 14

TGAGAAGGCATACATTATGAGAGATATGAAGAAGGAAAAGGAGCTAGGTTTTAGCATGCCTGTCTCACTTAGCAGAGCCCGCAAAAAACCATTGCTTCAGgttttcctct ... ttcgtttcagGGTAGAAGAGTATTAATCACACCAAACGCCAAGCCTAGCAAGGAGCTTCTGAAGAGTTTAGTAGTTGCAGCTCACGGTCAG

Block sizes: 43 (upstream exon), 43 (downstream exon)
Score: 5