ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CAAATCAGAGGGAAACCACTGTTATGTGGAGTAAATCCACTGGCCGTCCACTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttctttttatcattcactcgttttattaacattcgatatcatcaaatcttctcatcatgcttcaacaacacag

Basic information

species	Zea mays
transcript	GRMZM2G023163_T01
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G023163_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os04g55410.1 (Oryza sativa), 5'ss of exon 2

CAAATCAGAGGGAAACCACTGTTATGTGGAGTAAATCCACTGGCCGTCCACTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcg-ccttctttttatcattcactcgttttattaacattcgatatcatcaaatcttctcatcatgcttcaacaacacag
||||||||||||||||||||||||||||||| |||||||| |||| ||| || || ||||| ||||| |||||||||||||||||||||||  ||||||||||  || | | |  |||||    | |   ||| |   ||  ||||  |  | | ||||||| ||| ||  ||    |||
CAAATCAGAGGGAAACCACTGTTATGTGGAGCAAATCCACAGGCCTTCCTCTATACAATGCCATTGTATGGATGGATGCTCGCACAAGCCCCATTTGCAGgtcttccctttgtccatcat--gtttgacatatcagtgtt--atatagtgcagttttctcataatgttt-gacctttcag

upper sequence: GRMZM2G023163_T01 (Zea mays), 5'ss of exon 2
lower sequence: AT1G80460.1 (Arabidopsis thaliana), 5'ss of exon 2

CAAATCAGAGGGAAACCACTGTTATGTGGAGTAAATCCACTGGCCGTCCACTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttctttttatcattcactcgttttattaacattcgat--atcatcaaatcttctcatcatgcttcaacaacacag---------------------------------------
|| ||||||| || || |||||| | ||||| ||||||||||||| ||| ||  | || |||||||| |||||||||||||| || ||| |  | |||||||      ||||   | ||    |  |||| ||    |  | |   ||  | |   |  |||  ||   | |   |    |                                       
CAGATCAGAGAGAGACTACTGTTGTTTGGAGCAAATCCACTGGCCTTCCTCTCCACAAGGCTATTGTCTGGATGGATGCTCGTACCAGCTCCATCTGCAGgtacataactttcattgatctctttcttttcttcttgtatggtttgtctactatcatcttcaggattgctgagggatttggtttctgatgaaagctttctttggtgatccttaattacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|71766456|gb|DR964393.1|DR964393

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

EST: gi|88753132|gb|DY537273.1|DY537273

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCAC
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCAC

EST: gi|20300207|gb|BQ163150.1|BQ163150

EST:     CTGTATAATGCCATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGAACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

EST: gi|94475699|gb|EB704657.1|EB704657

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

EST: gi|28187593|gb|CB179203.1|CB179203

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGA
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGA

EST: gi|101392646|gb|EB818253.1|EB818253

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

EST: gi|22490347|gb|BU050270.1|BU050270

EST:     CTGTATAATGCCATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGAACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

EST: gi|211457288|gb|FK983850.1|FK983850

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGAYTGGAAAATGAGCTGTCAGGCGGTAGGACC-ACTTCGTGGAGA
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGA

EST: gi|19057965|gb|BM736632.1|BM736632

EST:     CTGTATAATGCCATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGAACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

EST: gi|211457293|gb|FK983855.1|FK983855

EST:     CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAG                         GAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG
genomic: CTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 46 (downstream exon)
Score: 67

CAAATCAGAGGGAAACCACTGTTATGTGGAGTAAATCCACTGGCCGTCCACTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttct ... aacaacacagGAGATTGGAAAATGAGCTGTCAGGCGGTAGGACCCACTTCGTGGAGACATGCGGGTTGCCAATCAGTACCTATTTCAGTGCTCTGAAATTATTATGGTTG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 96

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 75

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 179

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CAAATCAGAGGGAAACCACTGTTATGTGGAGTAAATCCACTGGCCGTCCACTGTATAATGCTATTGTGTGGATGGATGCTCGCACAAGCCCTGTTTGCAGgtcgccttctttttatcattcactcgttttattaacattcgatatcatcaaatcttctcatcatgcttcaacaacacag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GATGGA