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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgtttctttttttttttaactctttcaattaagtgaccaaatgagggacacttgccccctagattaaatttatactgtttctgtttgttattc...

Basic information

species Zea mays
transcript GRMZM2G022563_T01
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G022563_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os04g55060.1 (Oryza sativa), 5'ss of exon 2
ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgtttc--tttttttttttaactctttcaattaagtgaccaaatgagggacacttg--ccccctagat-taaatttatactgtttctgtttgttattc
 | |||||||||||||||||||||||||| || |||||||||||||||||||||||||| || || || ||||||||||| || ||||||||||||||||||| |  |  |||   || ||   |  | | |    |  |  |      || || | || |||  || |||  || |  ||  ||| |   | | | ||      
TCGCTTAGCGTTTCAAATGATGACCTTTCTAAAATAGTTGAAACATCAGATGAATGGATCGCAGCACGGACTGGGATTCGGAATAGGCGAGTTCTTTCAGgtacttctagttcaccttattccatcgagtttcatgacaagttagtttcattagcgtcagttgtgcctcctctatgtggatgcataataaatatttgtgg-----
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149099166|gb|EE185191.2|EE185191
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAC                         GAGATGAAACATTGCGGGGACTCTCGATACA
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACA
EST: gi|71430696|gb|DR811746.1|DR811746
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|76921158|gb|DV168472.1|DV168472
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCANAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|71446997|gb|DR828047.1|DR828047
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|89756348|gb|DY686398.1|DY686398
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCANAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|71429949|gb|DR810999.1|DR810999
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|76291106|gb|DV030674.1|DV030674
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|149095842|gb|EE181493.2|EE181493
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|149105057|gb|EE290536.2|EE290536
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|78121840|gb|DV540224.1|DV540224
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|148935022|gb|EC879064.2|EC879064
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
EST: gi|101397488|gb|EB820667.1|EB820667
EST:     TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAG                         GAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG
genomic: TGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 98

ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTGAGATGGCCGGAGTAAAAGCTGAAGATGTCGACCTCGTTCTTCTTTGTAC


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 70

ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTGAGATGGCCGGAGTAAAAGCTGAAGATGTCGACCTCGTTCTTCTTTGTAC


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 51

ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTGAGATGGCCGGAGTAAAAGCTGAAGATGTCGACCTCGTTCTTCTTTGTAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 168

ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgt ... aatgtgacagGAGATGAAACATTGCGGGGACTCTCGATACAAGCAGCTCAAAGGGCACTTGAGATGGCCGGAGTAAAAGCTGAAGATGTCGACCTCGTTCTTCTTTGTAC


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ACACTTAGCGTTTCAAATGATGACCTTTCGAAGATAGTTGAAACATCAGATGAATGGATTGCGGCTCGAACTGGGATTCGTAACAGGCGAGTTCTTTCAGgtaatgatgtttctttttttttttaactctttcaattaagtgaccaaatgagggacacttgccccctagattaaatttatactgtttctgtttgttattc

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgccccc