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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag

Basic information

species Zea mays
transcript GRMZM2G011923_T01
intron # 2
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os05g41930.1 (Oryza sativa), 5'ss of exon 2
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggta-actgttctggaa---aatcagttttgatcctagcgtatatactaatttggtaa-----tggtgccttaagatctgttgctcttctgcag
|| || || |||||||| || | ||| ||||| || ||||| || |||||||| |||||||| || |||||||||||||| ||||||||||||||||||||||||| | | ||| || |||| ||| |||| ||| | | ||||| | | ||| | || | | || || || ||| || | | | || | ||||| ||||||||
CCAACAAGTGAGATGCTACCAAGGAATGAAGTGCTCGGTGGATATATCTTTGTTTGCAACAATGACACCATGCAGGAGGATCTCAAGAGGCAGCTTTTTGgtatgtgatcc-------aacttaccttatcttctgaatcatcacttggcatattgttccgagaataaattcatgctgctatttacgatgatcaatatcaaataaccttctgatatataatcatatattgctgttctgcag

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: AT3G27090.1 (Arabidopsis thaliana), 5'ss of exon 2
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag
|| | |||| || |||| ||| ||||| |||||||| ||||||||||| |||||||| ||||| ||||||||||| | || | || || ||||||| ||| | | | || || | || || ||||| | | |||| | |||| | | | || | || |
CCTGCTTCGGAGACTCTTCCGAGGAATGAAGTTCTTGGTGGTTACATCTTTGTTTGCAACAATGATACTATGCAGGAGGATATGAAACGTCACCTCTTTGgta-----atttcccaatacccttttctagtgttttactgat---attcaaactg--caagtaaataactttt-----tgactttcatcattgttcag----------------------------------

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: Vv14s0068g02040.t01 (Vitis vinifera), 5'ss of exon 2
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgta----tgtctttttt--tttggatcttgc-catatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag
|| | |||| || || || || || ||||| |||||||||||||| || ||||| |||||||||||||| || | ||| | || || ||||||| || ||| || ||| || | | ||| | ||| | | | ||| ||| |||| || | | | | || | ||||| || | ||
CCTGCTTCAGAGACACTCCCTCGAAATGAGGTTCTTGGAGGGTACATCTTTGTGTGTAACAATGATACCATGCAGGAAGATTTGAAGCGACAACTATTTGgtaatagtgattttcttgctttatattctcctcatttaacttatgtactttgctgggaacagttcaggcattttacactca---ttaactcatataata-tgtg-----------------------------------

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: PP1S4_323V6.1 (Physcomitrella patens), 5'ss of exon 1
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggtaact-gttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag
||| | || | || || || | | || || ||||| || |||||||| ||||| ||||||||||||||||| || | || ||||| |||| || ||| | | | | | | | | | | || || || ||| | || || | | | | ||||
CCGTCAGCTGAAGTCCTTCCTCGAAATGTCACATTGGGGGGTTACATATTCGTCTGCAATAACGACACCATGCAGGAGGACCTTAAACGCCAACTTTTCGgtacgtttttactgtcatctgatttttcacttcagatcttactagagaatgacaagtagcacgaattcatctatggctcttgtgaaggtcataatcaaaa---------------------------------

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ22023 (Selaginella moellendorffii), 5'ss of exon 1
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag
|| || |||||||| || ||| ||| |||||||| || |||||||| |||||||| ||||||||||| |||||||||||| | ||||| ||||||| ||| | | | | || | | | | ||| | | | || | | || || | | ||
CCACCGTCGGAGATGCTACCACGGAATGAAACGCTTGGTGGCTATATCTTTGTTTGCAACAATGATACCATGCAAGAGGACCTCAAGCGCCAGCTCTTTGgtaagtcctctctcgtt-atttctctgtctttcttcgtagctatgactgaaagcttgtttttttttcttttgcgctttcag---------------------------------------------------

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ24245 (Selaginella moellendorffii), 5'ss of exon 1
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctt---tttttttggatcttg---ccatatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaag---atct-gttgctcttctgcag--------
|| | ||||| ||| || || ||| ||||| |||||||| ||||| || |||||||||||||||||||| || || |||||| | || || || |||| ||| | ||| | | ||| | || || | | | | || || | || | | || ||| || | | ||| |||| ||| ||| || |
CCTCCATCCGAGACGCTTCCCCGGAGCGAGGTCCTCGGTGGGTATATCTTCGTGTGCAACAACGATACCATGCAAGAAGATCTCAAGCGCCAACTCTTCGgtaaaccttacatcccgttgaaacaaaactccaccatctccattttttctcttgctctcggccataaaacgctcgt--aagttcttttttcttctcttgttttttatttttttctttcctttatctcgtttctcacctagaacttcgcag

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ35508 (Selaginella moellendorffii), 5'ss of exon 1
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttg---------gatcttgccatatcccttgaggaatcaaatggtaactgtt-----ctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag-----------
|| | ||||| ||| || || ||| ||||| |||||||| ||||| || |||||||||||||||||||| || || |||||| | || || || |||| ||| | | | |||| ||| || | | | || | || | |||| | || || | | |||||| | | | |||| | || ||| ||| || |
CCTCCATCCGAGACGCTTCCCCGGAGCGAGGTCCTCGGTGGGTATATCTTCGTGTGCAACAACGATACCATGCAAGAAGATCTCAAGCGCCAACTCTTCGgtaaaccttacatcccgttgaaacaaaactccaccatctccattttttctcttgctctcggccataaaacgctcgtaagtttttttttcttctcttgttttt-ttaatttttt--tctttcctttatctc-gtttctcacctagaagaacttcgcag

upper sequence: GRMZM2G011923_T01 (Zea mays), 5'ss of exon 2
lower sequence: EFJ18285 (Selaginella moellendorffii), 5'ss of exon 1
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag
|| || |||||||| || ||| ||| |||||||| || |||||||| |||||||| ||||||||||| |||||||||||| | ||||| ||||||| ||| | | | | | || | | | | | | || | || | | || || | | ||
CCACCGTCGGAGATGCTACCACGGAATGAAACGCTTGGTGGCTATATCTTTGTTTGCAACAATGATACCATGCAAGAGGACCTCAAGCGCCAGCTCTTTGgtaagtcctctctcgttttttttctgtctttcttcgtagctatgactgaaagcttgttttttttttcttttgcgctttcag---------------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|22490259|gb|BU050182.1|BU050182
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCAAGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149078427|gb|EE167203.2|EE167203
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|29544431|gb|CB604811.1|CB604811
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149080351|gb|EE169368.2|EE169368
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|31351065|gb|CD435422.1|CD435422
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|31352279|gb|CD436636.1|CD436636
EST:     CATGCAGGAGGACCTCAAGAGGCAGCTTTTT-                         GATTTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTG
genomic: CATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGA-TTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTG
EST: gi|149082226|gb|EE171409.2|EE171409
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTC
EST: gi|29544031|gb|CB604411.1|CB604411
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149107939|gb|EE292295.2|EE292295
EST:     GTCTGNCA-CAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GAT-GCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: GTCTG-CAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|87152915|gb|DY397704.1|DY397704
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCAAGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|31351868|gb|CD436225.1|CD436225
EST:     TGTCTGCAACAACGATACCATGGAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACCATAT
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATAT
EST: gi|31351326|gb|CD435683.1|CD435683
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|148982112|gb|EE028161.2|EE028161
EST:     GTTTTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: G-TCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149102032|gb|EE287514.2|EE287514
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|37399688|gb|CF637177.1|CF637177
EST:     CATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCAAGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: CATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149107040|gb|EE188934.2|EE188934
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGGCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149111905|gb|EE191062.2|EE191062
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|40303492|gb|CK347879.1|CK347879
EST:     TGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCAAGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|93282541|gb|EB674805.1|EB674805
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGGCA-TTACTCCTGGCCTG
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGG-CAATTACTCCTGGCCTG
EST: gi|31352510|gb|CD436867.1|CD436867
EST:     CAAGAGGCAG-TTTTTT                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: CAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|148982113|gb|EE028162.2|EE028162
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|149107300|gb|EE189181.2|EE189181
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGG
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGG
EST: gi|211413346|gb|FL280968.1|FL280968
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GATTGCCAGCAAGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagGATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTGC
EST: gi|78087741|gb|DV516134.1|DV516134
EST:     TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTG                         GGAT-GCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTG
genomic: TGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtcttt ... tcttctgcagG-ATTGCCAGCACGATATCGTGATTCAGTCCGGGCAATTACTCCTGGCCTG

Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CCGACGAGCGAGATGCTGCCGAAGAACGAAGTCCTTGGTGGGTACATCTTTGTCTGCAACAACGATACCATGCAGGAGGACCTCAAGAGGCAGCTTTTTGgtatgtctttttttttggatcttgccatatcccttgaggaatcaaatggtaactgttctggaaaatcagttttgatcctagcgtatatactaatttggtaatggtgccttaagatctgttgctcttctgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT