ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TGGGATTTTGAAGAACACAATTTATGATTACTTTGACAAGAACTTCACTGGGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatgaggtgcaaattgcatgtagtaatattgaatcgatatatgctattttgcatctactaataatcaactgcagtagtagtattacccttaaggcctta...

Basic information

species	Zea mays
transcript	AC211349.4_FGT001
intron #	2
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: AC211349.4_FGT001 (Zea mays), 5'ss of exon 2
lower sequence: LOC_Os12g34860.1 (Oryza sativa), 5'ss of exon 2

TGGGATTTTGAAGAACACAATTTATGATTACTTTGACAAGAACTTCACTGGGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatgaggtgcaaattgcatgtagtaatattgaatcgatatatgctattttgcatctactaataatcaactgcagtagtagtattacccttaaggcctta--------------------------------------------------------------------------------------------
 |||||| |||||||||| ||||| || |||||||||||||| ||| ||||| | ||| ||||||| |||||||| ||||||||||||||| ||||||||||||||| |   | |||    |  |  | || ||  | | ||| |  |      | | || ||  || ||||   ||||    ||  | ||  |  |||                                                                                             
AGGGATTCTGAAGAACACTATTTACGACTACTTTGACAAGAATTTCTCTGGGAACTTTAACAAGTTCGATGACCTCTGCCCTCTTGTTTCTATCAAGCAGgtatgagttagcagttg-gcctgatg-tgttcaactgct-tattccttcccttttgtgctgatt-tccactgggctagtgaatttcgctttgtgcacttgtttttctttttttcagattctcgcacaccttgtgtaacagtgtactttgaaccatatgattattgctgaccccaaggtcatgcttgctgcag

upper sequence: AC211349.4_FGT001 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA18G52890.1 (Glycine max), 5'ss of exon 3

TGGGATTTTGAAGAACACAATTTATGATTACTTTGACAAGAACTTCACTGGGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatg-aggtgcaaattgcatgtagtaatattgaatcgatatatgctattttgcatctactaataatcaactgcagtagtagtattaccc---ttaaggcctta-
||||||  |||||||  |||||||||| || |||||||   | |   |     ||||  ||||||||||||| ||||| ||| ||||||| || ||||||||||| ||    |||||| | | | || | |    | |     |  |||  |||||  |   ||  | | |||      | || | || |   ||||  |  || 
TGGGATACTGAAGAATGCAATTTATGAGTATTTTGACACACATTATTCAAATAAGTTCAACAAGTTTGATGATCTTTGTCCTATTGTTTCAGTGAAGCAGgtatggagaaataaattg-aagaattagtttccctttgcctccta-tatgatgcattgat--atttttagctgttact-ttgtttcacacatgttaatacactat

upper sequence: AC211349.4_FGT001 (Zea mays), 5'ss of exon 2
lower sequence: GLYMA02G10060.1 (Glycine max), 5'ss of exon 2

TGGGATTTTGAAGAACACAATTTATGATTACTTTGACAAGAACTTCACTGGGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatg-aggtgcaaattgcatgtagtaatattgaatcgatatatgctattttgc-atctactaataatcaactgcagtagta-gtattaccc---ttaaggcctta--
||||||  |||||||  | || || || || |||||||  || |   |     ||||  ||||||||||||| ||||| ||| ||||||| || ||||||||||| |     ||||||        || | | | | |   | ||||  | |   ||  |  |||| |         || |  || | || |   | ||  |  ||  
TGGGATACTGAAGAATGCGATATACGAGTATTTTGACACTAATTATTCAAATAAGTTCAACAAGTTTGATGATCTTTGTCCTATTGTTTCAGTGAAGCAGgtatggaaaaataaattg--------aagaattagttgccctttgctcctatatgatgcatcaatagttttagatgttactttgtttcacacatgtcaatacactatt

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|4753305|gb|AI657210.1|AI657210

EST:     GGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAG                         AATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAAC
genomic: GGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatgaggtg ... tttgctgcagAATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAAC

EST: gi|67041313|gb|CO467568.1|CO467568

EST:     GTTTGAC-A-TTTCGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAG                         AATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAAC
genomic: GTTTGACAAGTTT-GATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatgaggtg ... tttgctgcagAATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAAC

EST: gi|60340116|gb|DN207089.1|DN207089

EST:     AGTTTGACAAGTTTGATGACCTATTGCCCTCTTGTTTCTGTCAAGCAG                         AATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAAC
genomic: AGTTTGACAAGTTTGATGACCT-TTGCCCTCTTGTTTCTGTCAAGCAGgtatgaggtg ... tttgctgcagAATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 81

TGGGATTTTGAAGAACACAATTTATGATTACTTTGACAAGAACTTCACTGGGCAGTTTGACAAGTTTGATGACCTTTGCCCTCTTGTTTCTGTCAAGCAGgtatgaggtg ... tttgctgcagAATTTTGATGATGTCTTGGTCCCTTCTGACCATGTAAGCCGGAGTTACAACGACACATATTATGTTGATGGTCAAACAGTCTTAAGGTGTCATACCAGTG

Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 107

Block sizes: 42 (upstream exon), 49 (downstream exon)
Score: 48