ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

CAATAATTTTGTTGATGCCGGTGCCCTCTCTGAGTTGATCGTAGCCTTTTCTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAGgtaatccgattaccattgctctaatcatgtttcgttgggaaggcgtatatttcccctccatttcaccatcgttacgtgaccttccccag

Basic information

species	Zea mays
transcript	GRMZM2G168858_T01
intron #	16
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G168858_T01 (Zea mays), 5'ss of exon 16
lower sequence: LOC_Os09g38620.1 (Oryza sativa), 5'ss of exon 16

CAATAATTTTGTTGATGCCGGTGCCCTCTCTGAGTTGATCGTAGCCTTTTCTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAGgtaatccgattaccattgctctaatcatgtttcgt-tgggaaggcgtatatttcccctccatttcaccatcgttacgtgaccttccccag
||| |||||||||||  ||||||| || ||||| ||||| |||||||||||||||||||||||| |||| |||||||||||||||||||||||  | ||||||     | |   ||   | |     |||||  | |  |  |  || | ||    | |  ||||||||| | |  || |  |||||   
CAACAATTTTGTTGAGACCGGTGCGCTGTCTGAATTGATTGTAGCCTTTTCTCGTGAGGGTCCTTCAAAGGAGTATGTTCAGCACAAAATGGCAGAGAAGgtacatgaaatg--atcctttt-----tgtttaatataaggtgtagtgttttatttccctttttcaccattgctttgtaatattcccag-

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211083504|gb|FL101467.1|FL101467

EST:     CTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAG                         GCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTATATTTATGTTTGC
genomic: CTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAGgtaatccgat ... ccttccccagGCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTACATTTATGTTTGC

EST: gi|149075934|gb|EE164497.2|EE164497

EST:     CTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAG                         GCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTACATTTATGTTTGC
genomic: CTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAGgtaatccgat ... ccttccccagGCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTACATTTATGTTTGC

EST: gi|148929251|gb|EC874912.2|EC874912

EST:     CTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAG                         GCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTACATTTATGTTTGC
genomic: CTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAGgtaatccgat ... ccttccccagGCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTACATTTATGTTTGC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 33

CAATAATTTTGTTGATGCCGGTGCCCTCTCTGAGTTGATCGTAGCCTTTTCTCGTGAGGGTCCTACAAAAGAGTATGTTCAGCACAAAATGGCGCAAAAGgtaatccgat ... ccttccccagGCTGCCGAGCTTTGGAGCATCATTTCTCAGGGCGGCTACATTTATGTTTGCGGTGACGCAAAAGGCATGGCTAGAGATGTCCATCGCACGCTACATACCA

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 104

Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 81